Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Simultaneous Extraction and Separation of Oil and Azadirachtin from Seeds and Leaves of Azadirachta indica using Binary Solvent Extraction

  • Subramanian, Sheela (Branch Campus Malaysian Institute of Chemical and Bio-Engineering Technology, Green Chemistry & Sustainable Engineering Technology Cluster, Universiti Kuala Lumpur) ;
  • Salleh, Aiza Syuhaniz (Branch Campus Malaysian Institute of Chemical and Bio-Engineering Technology, Green Chemistry & Sustainable Engineering Technology Cluster, Universiti Kuala Lumpur) ;
  • Bachmann, Robert Thomas (Branch Campus Malaysian Institute of Chemical and Bio-Engineering Technology, Green Chemistry & Sustainable Engineering Technology Cluster, Universiti Kuala Lumpur) ;
  • Hossain, Md. Sohrab (Division of the Environmental Technology, School of Industrial Technology, Universiti Sains Malaysia)
  • Received : 2018.07.04
  • Accepted : 2018.12.18
  • Published : 2019.06.30
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Abstract

Conventional extraction of oil and azadirachtin, a botanical insecticide, from Azadirachta indica involves defatting the seeds and leaves using hexane followed by azadirachtin extraction with a polar solvent. In order to simplify the process while maintaining the yield we explored a binary extraction approach using Soxhlet extraction device and hexane and ethanol as non-polar and polar solvents at various ratios and extraction times. The highest oil and azadirachtin yields were obtained at 6 h extraction time using a 50:50 solvent mixture for both neem leaves (44.7 wt%, $720mg_{Aza}/kg_{leaves}$) and seeds (53.5 wt%, $1045mg_{Aza}/kg_{leaves}$), respectively.

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References

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