DOI QR코드

DOI QR Code

전립선비대증의 어성초추출물에 의한 경구투여 효과

Effect of Oral Administration of Houttuynia Cordata Extract on Benign Prostatic Hyperplasia

  • 송원영 (한국국제대학교 식품영양학과) ;
  • 최정화 (한국국제대학교 식품영양학과)
  • Song, Won-Yeong (Department of Food and Nutrition, International University of Korea) ;
  • Choi, Jeong-Hwa (Department of Food and Nutrition, International University of Korea)
  • 투고 : 2019.02.15
  • 심사 : 2019.06.14
  • 발행 : 2019.06.30

초록

본 연구는 전립선비대증이 노화 및 남성호르몬 과다분비로 인해 유발된다는 가설을 바탕으로 순화기간을 마친 12주령이 된 수컷 쥐에 testosterone을 30일간 피하주사하여 노화 및 testosterone 분비 증가로 인한 전립선비대증 유발 모델 rat을 제작하였다. 실험군은 총 4군으로 각 군당 10마리씩 정상군(N 군), 음성대조군(DO 군), 어성초 추출물 투여군(HO 군) 및 양성물질 finasteride 투여군(FO 군)으로 나누어서 진행하였다. 고환절제술을 한 후 testosterone을 투여한 결과 N군에 비해 나머지 세 군, DO, HO 및 FO군 모두에서 체중이 유의적으로 감소하였다. 전립선의 부피는 N군에 비해서 DO군에서 유의적으로 증가하였으며, HO군과 FO군에서는 DO군에 비해 유의적으로 감소되는 경향을 나타내었다. 전립선의 무게 또한 HO군 및 FO군에서 DO군에 비해 유의적으로 감소되었다. 체중 당 전립선의 비율은 N군에 비해 DO군, HO군 및 FO군에서 유의적으로 증가하였으며, 특히 DO군에 비해 HO 및 FO군은 각각 유의적인 감소를 나타내었다. 혈중 testosterone 및 DHT의 농도는 N군에 비해 모든 군에서 유의적으로 증가되었으나, DO군에서 증가된 혈중 testosterone 및 DHT의 농도는 어성초 추출물 및 finasteride의 공급으로 유의적으로 감소되는 경향이었다. 어성초 추출물의 공급으로 인한 조직병리학적 변화양상을 관찰한 결과 어성초추출물 공급군에서는 DO군에 비해 상피세포, acinar gland, 관상피 세포, 세포핵, 분비선들의 변형 및 위축이 감소하여 정상적인 형태를 나타내고, 양성대조군으로 사용된 finasteride 처리군의 형상과 비슷한 양상을 나타내었다. 어성초 추출물에 대한 이러한 효과는 향후 분자생물학적으로 기전연구들이 더욱 필요하지만 본 결과로 미루어 어성초 추출물 내 함유된 다양한 페놀 및 플라보노이드와 같은 생리활성 성분들은 지금까지 알려져 있던 발모 및 여드름 개선뿐만 아니라 이와 유사한 기전을 가지고 있는 전립선비대증 개선에도 효과적으로 기여할 수 있을 것이라 기대된다.

Benign prostatic hyperplasia (BPH) is the most common urogenital disorder in men, benign tumor and is a typical disease deteriorating the quality of old men's lives, and its prevalence increases with age. Though the molecular pathogenesis of BPH has not yet been clearly revealed, it is known that the variation and aging of the endocrine including sex hormone may cause BPH. Especially the hypertrophy of the prostate cell by the formation of the excessive dihydrotestosterone (DHT) is estimated to cause BPH. If testosterone exists excessively in blood, a lot of DHT is produced in prostate by $5{\alpha}-reductase$. Thus, in this study we tried to analyze haematological change and histopathological change by using the model rat with BPH caused by hypodermic injection of testosterone to prove the effect of Houttuynia cordata extracts on BPH. Rats were divided into four experimental groups: no treatment group (N), the testosterone injection and D.W treatment group (DO), the testosterone injection and Houttuynia cordata treatment group (HO) and testosterone injection and finasteride treatment group (FO). Prostate weight, volume and weight ratio in the HO and FO groups were significantly lower than the DO group. Testosterone and DHT levels in the HO group were significantly lower than the DO group. The HO and FO groups showed trophic symptoms and were lined by flattened epithelial cells, thus, the stromal proliferation is relatively low as compared to the DO group. These results suggest that Houttuynia cordata may control benign prostatic hyperplasia.

키워드

SMGHBM_2019_v29n6_705_f0001.png 이미지

Fig. 1. Testosterone levels of each experimental group.

SMGHBM_2019_v29n6_705_f0002.png 이미지

Fig. 2. Dihydrotestosterone (DHT) levels of each experimental group.

SMGHBM_2019_v29n6_705_f0003.png 이미지

Fig. 3. H&E stains of prostate tissue from the rats (×40).

Table 1. Body weight gains of each experimental group

SMGHBM_2019_v29n6_705_t0001.png 이미지

Table 2. Prostate volume, weight and prostate weight ratio of each experimental group

SMGHBM_2019_v29n6_705_t0002.png 이미지

참고문헌

  1. Asada, Y., Sonoda, T., Ojiro, M., Kurata, S., Sato, T., Ezaki, T. and Takayasu, S. 2001. 5 Alpha-reductase type 2 is constitutively expressed in the dermal papilla and connective tissue sheath of the hair follicle in vivo but not during culture in vitro. J. Clin. Endocrinol. Metab. 86, 2875-2880. https://doi.org/10.1210/jcem.86.6.7545
  2. Babinski, M. A., Chagas, M. A., Costa, W. S. and Sampaio, F. J. 2003. Prostatic epithelial and luminal area in the transition zone acini: morphometric analysis in normal and hyperplastic human prostate. BJU Int. 92, 592-596. https://doi.org/10.1046/j.1464-410X.2003.04433.x
  3. Bisson, J. F., Hidalgo, S., Rozan, P. and Messaoudi, M. 2007. Therapeutic effect of ACTICOA powder, a cocoa polyphenolic extract, on experimentally induced prostate hyperplasia in Wistar-Unilever rats. J. Med. Food 10, 628-635. https://doi.org/10.1089/jmf.2006.242
  4. Carson, C. and Rittmaster, R. 2003. The role of dihydrotestosterone in benign prostatic hyperplasia. Urology 61, 2-7. https://doi.org/10.1016/S0090-4295(03)00045-1
  5. Cho, H. D., Kim, D. H., Kim, M. G., Lee, Y. S. and Seo, K. I. 2018. Biological activity of Oenothera Biennis seed extracts. J. Life Sci. 28, 1048-1055. https://doi.org/10.5352/JLS.2018.28.9.1048
  6. Cho, S. H., Han, Y. H. and Kim, Y. S. 2010. Effects of bee venom herbal acupuncture on experimental rat model of benign prostatic hyperplasia. J. Kor. Orient. Intern. Med. 31, 166-176.
  7. Choi, Y. S., Lee, H. H., Lee, B. H. and Lee, S. Y. 1998. Effects of extracts of Houttuynia cordata Thunb on the level of lipids and lipid peroxidation in the liver and serum of cholesterol-fed rats. Kor. J. Plant. Res. 11, 173-178.
  8. Coffey, D. S. and Walsh, P. C. 1990. Clinical and experimental studies of benign prostatic hyperplasia. Urol. Clin. North. Am. 17, 461-475. https://doi.org/10.1016/S0094-0143(21)00960-5
  9. Du, I. S., Seo, Y. J., Woo, W. H., Oh, H. C., Park, M. C. and Kim, N. K. 2004. Clinical study on the effect of external treatment of 5% Herba Houttuyniae extract solution on the acne. J. Physiol. Pathol. Kor. Med. 18, 612-620.
  10. Hong, Y. H., Bae, S. H. and Suh, H. J. 2015. Effect of herbal complex extract including Houttuynia cordata Thunb on hair growth promotion in C57BL/6 Mice. Kor. J. Aesthet. Cosmetol. 13, 321-329.
  11. Kang, H. S., Kim, G. Y., Jung, I., Oh, S. D., Kim, C. H., Shim, B. S., Park, K. H. and Oh, S. J. 2007. The effect of the compound of tomato extract to the prostatic cancer cell and the prostate of the rat model of benign prostatic hyperplasia. Kor. J. Pharmacogn. 38, 197-203.
  12. Kaufman, K. D., Deveillez, R. and Roberts, J. 1994. A12 month pilot clinical study of the effects of finasteride on men with male attern baldness. J. Invest. Dermatol. 102, 615.
  13. Kim, H. M., Han, S. B., Oh, G. T., Kim, Y. H., Hong, D. H., Hong, N. D. and Yoo, I. D. 1996. Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Int. J. Immunopharmacol. 18, 295-303. https://doi.org/10.1016/0192-0561(96)00028-8
  14. Kim, J. S., Han, Y. H. and Kim, Y. S. 2009. The effects of Scutellaria baicalensis on experimental rat model of benign prostatic hyperplasia. Kor. J. Orient. Intern. Med. 30, 327-337.
  15. Kim, J. H., Cho, H. D., Hong, S. M., Lee, J. H., Lee, Y. S., Kim, D. H. and Seo, K. I. 2016. Antioxidant and anti proliferating effects of Setaria italica, Panicum miliaceum and Sorghum bicolor extracts on prostate cancer cell line. Kor. J. Food Preserv. 23, 1033-1041. https://doi.org/10.11002/kjfp.2016.23.7.1033
  16. Kim, S. D., Lee, B. H., Sohn, D. W., Cho, Y. H., Lee, S. M., Kim, J. O. and Lim, S. W. 2008. The effect of herbal formulation KH-305. mainly consisted of Rubus coreanus on benign prostatic hyperplasia-induced rat. Kor. J. Pharmacogn. 39, 80-85.
  17. Kim, Y. N., Kim, M. S., Chun, S. S and Choi, J. H. 2013. Effect of Phellius linteus water extract on benign prostatic hyperplasia. Nutr. Res. Pract. 7, 172-177. https://doi.org/10.4162/nrp.2013.7.3.172
  18. Lee, S. J., Choi, H. R., Lee, J. H., Kwon, J. W., Lee, H. K., Jeong, J. T. and Lee, T. B. 2014. Effects of unripe black raspberry extracts on prostate cancer cell line and rat model of benign prostatic hyperplasia. J. Kor. Soc. Food Sci. Nutr. 43, 507-515. https://doi.org/10.3746/jkfn.2014.43.4.507
  19. Lim, M. R. and Kang, S. M. 2009. Improvement effect of Salicornia herbacea L. diet on the acne skin. J. East Asian Soc. Dietary Life 19, 750-760.
  20. Moon, J. M., Sung, H. M., Jung, H. J., Seo, J. W. and Wee, J. H. 2017. In vivo evaluation of hot water extract of Acorus gramineus root against benign prostatic hyperplasia. BMC Complement Altern. Med. 22, 414.
  21. Naresh, K. N., Aparna, B. Dr. and Veeresh, B. 2013. Protective effect of naringin on testosterone induced benign prostatic hyperplasia in rats. Intern. J. Pharm. Res. Scholars 2, 239-244.
  22. Park, D. J., Kang, S. H. and Cho, Y. H. 2006. The anti hyperplastic effect of oral catechin ingestion in a rat model of benign prostatic hyperplasia. Kor. J. Urol. 47, 1289-1293. https://doi.org/10.4111/kju.2006.47.12.1289
  23. Park, H. S., Chang, N. S., Kim, E. J., Yun, H. N., Lee, H. J., Lee, B. E., Kim, H. H., Kwon, S. W. and Jeong, Y. J. 2004. Association between nutrient intakes and benign prostate hyperplasia. Kor. J. Nutr. 37, 801-808.
  24. Pyo, J. S. and Cho, W. J. 2017. Systematic review and meta-analysis of prostatic artery embolisation for lower urinary tract symptoms related to benign prostatic hyperplasia. Clin. Radiol. 72, 16-22. https://doi.org/10.1016/j.crad.2016.10.009
  25. Richard, A., Hiipakka, R. A., Zhong, H. Z., Dai, W., Dai, Q. and Liao, S. 2002. Structure-activity relationships for inhibition of human $5{\alpha}$-redutases by polyphenols. Biochem. Pharmacol. 63, 1165-1176. https://doi.org/10.1016/S0006-2952(02)00848-1
  26. Smith, R. D. and Patel, A. 2011. Transurethral resection of the prostate revisited and updated. Curr. Opin. Urol. 21, 36-41. https://doi.org/10.1097/MOU.0b013e3283411455
  27. Song, H. J. and Shin, M. K. 1987. Effects of Houttuyniae herba on immune responses and histological findings in mice bearing pneumonitis. Kor. J. Pharmacogn. 18, 216-232.
  28. Sreel, R. G. D. and Torrie, J. H. 1990. Principles and procedures of statistics. Mcgrow Hill, New York, NY, USA.
  29. Sung, N. J., Lee, S. J., Shin, J. H. and Kim, H. S. 1998. The effect of feeding juice and powder of Houttuynia cordata Thunb on serum lipids in rats. J. Kor. Soc. Food Sci. Nutr. 27, 1223-1229.
  30. Thigpen, A. E., Silver, R. I., Guileyardo, J. M., Casey, M. L., Mc-Connell, J. D. and Russell, D. W. 1993. Tissue distribution and ontogeny of steroid 5alpha-reductase isozyme expression. J. Clin. Invest. 92, 903-910. https://doi.org/10.1172/JCI116665
  31. Thorner, D. A. and Weiss, J. P. 2009. Benign prostatic hyperplasia: symptoms, symptom scores, and outcome measures. Urol. Clin. North. Am. 36, 417-429. https://doi.org/10.1016/j.ucl.2009.07.001