Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Ring Test as Acute Toxicity Test with Korean Freshwater Shrimp, Neocaridina denticulata using 3,4-Dichloroaniline

국내 서식 담수새우 새뱅이(Neocaridina denticulata)를 이용한 3,4-Dichloroaniline의 급성독성 교차시험(Ring test)

  • Shin, Yu-jin (Risk Assessment Division, National Institute of Environmental Research) ;
  • Lee, Jae-woo (Chemical Research Division, National Institute of Environmental Research) ;
  • Kim, Jieun (Risk Assessment Division, National Institute of Environmental Research) ;
  • Cho, Jaegu (Toxicological Research Center, Hoseo University) ;
  • Kim, Ja-Hyun (Korean Institute of Toxicology) ;
  • Kang, Minho (Neo Environmental Business Co.) ;
  • Kim, Kyungtae (Risk Assessment Division, National Institute of Environmental Research) ;
  • Kim, Pil-je (Risk Assessment Division, National Institute of Environmental Research) ;
  • Park, Kyunghwa (Risk Assessment Division, National Institute of Environmental Research)
  • 신유진 (국립환경과학원 위해성평가연구과) ;
  • 이재우 (국립환경과학원 화학물질연구과) ;
  • 김지은 (국립환경과학원 위해성평가연구과) ;
  • 조재구 (호서대학교 안전성평가센터) ;
  • 김자현 (안전성평가연구소 환경독성연구센터) ;
  • 강민호 (네오엔비즈) ;
  • 김경태 (국립환경과학원 위해성평가연구과) ;
  • 김필제 (국립환경과학원 위해성평가연구과) ;
  • 박경화 (국립환경과학원 위해성평가연구과)
  • Received : 2019.06.08
  • Accepted : 2019.06.20
  • Published : 2019.06.30
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Abstract

Objectives: For suitable risk management of the domestic aquatic environment, it is necessary to conduct toxicity tests using species native to Korea. In the present study, we performed toxicity ring tests using endemic freshwater arthropoda Neocaridina denticulata and evaluated its validity and reproducibility as an international standard test species. Methods: To evaluate the sensitivity levels of N. denticulata to hazardous chemicals, toxicity values for several chemicals were compared with other standard test species. Intra- and inter-laboratory acute toxicity tests were performed both within a single laboratory and among four laboratories respectively using 3,4-Dichloroaniline, which is generally used as a reference test substance in fish toxicity tests. In addition, intra- and inter-laboratory coefficient of variations (CVs) were calculated to evaluate reproducibility based on the estimated toxicity values. Results: The sensitivity of N. denticulata to several chemicals was found to be similar with D. manga, indicating that the species is valid as a test species. The CVs of the intra- and inter-laboratory tests were 22.946% with four qualified runs and 8.828% among the four laboratories, respectively. Conclusions: N. denticulata serves in an important role in the food chain of Korean aquatic ecosystems and also inhabits several other Asian countries. Since the validity and reproducibility of the species were confirmed as a toxicity test species in this study, further efforts are needed to establish N. denticulata as the international standard test species for the appropriate risk assessment of aquatic ecosystems at home and abroad.

Keywords

References

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