상하수도학회지 (Journal of Korean Society of Water and Wastewater)

  • 제33권3호
  • /
  • Pages.191-204
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  • 2019
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  • 1225-7672(pISSN)
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  • 2287-822X(eISSN)
대한상하수도학회 (The Korean Society of Water and Wastewater)
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초음파 조사에 의한 항생제 내성균 불활성화 및 감수성 변화

Comparison of inactivation and sensitivity of antibiotic resistance bacteria by ultrasound irradiation

  • 이성훈 (중앙대학교, 토목공학과) ;
  • 남성남 (중앙대학교, 토목공학과) ;
  • 오재일 (중앙대학교, 토목공학과)
  • Lee, Sunghoon (Department of Civil and Environmental Engineering, Chung-Ang University) ;
  • Nam, Seong-Nam (Department of Civil and Environmental Engineering, Chung-Ang University) ;
  • Oh, Jeill (Department of Civil and Environmental Engineering, Chung-Ang University)
  • 투고 : 2019.04.16
  • 심사 : 2019.05.10
  • 발행 : 2019.06.15
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초록

The 20-kHz ultrasonic irradiation was applied to investigate bacterial inactivation and antibiotic susceptibility changes over time. Applied intensities of ultrasound power were varied at 27.7 W and 39.1 W by changing the amplitude 20 to 40 to three bacteria species (Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus). By 15-min irradiation, E. coli, a gram-negative bacterium, showed 1.2- to 1.6-log removals, while the gram-positive bacteria, Enterococcus faecalis and Staphylococcus aureus, showed below 0.5-log removal efficiencies. Antibiotic susceptibility of penicillin-family showed a dramatic increase at E. coli, but for other antibiotic families showed no significant changes in susceptibility. Gram-positive bacteria showed no significant differences in their antibiotic susceptibilities after ultrasound irradiation. Bacterial re-survival and antibiotic susceptibility changes were measured by incubating the ultrasound-irradiated samples. After 24-hour incubation, it was found that all of three bacteria were repropagated to the 2- to 3-log greater than the initial points, and antibiotic inhibition zones were reduced compared to ones of the initial points, meaning that antibiotic resistances were also recovered. Pearson correlations between bacterial inactivation and antibiotic susceptibility showed negative relation for gram-negative bacteria, E. coli., and no significant relations between bacterial re-survival and its inhibition zone. As a preliminary study, further researches are necessary to find practical and effective conditions to achieve bacteria inactivation.

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참고문헌

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