Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Study on the correlation between the soil bacterial community and growth characteristics of wild-simulated ginseng(Panax ginseng C.A. Meyer)

토양세균군집과 산양삼 생육특성 간의 상관관계 연구

  • Kim, Kiyoon (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Um, Yurry (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Jeong, Dae Hui (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kim, Hyun-Jun (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Kim, Mahn Jo (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Jeon, Kwon Seok (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 김기윤 (국립산림과학원 산림약용자원연구소) ;
  • 엄유리 (국립산림과학원 산림약용자원연구소) ;
  • 정대희 (국립산림과학원 산림약용자원연구소) ;
  • 김현준 (국립산림과학원 산림약용자원연구소) ;
  • 김만조 (국립산림과학원 산림약용자원연구소) ;
  • 전권석 (국립산림과학원 산림약용자원연구소)
  • Received : 2019.08.22
  • Accepted : 2019.09.17
  • Published : 2019.09.30
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Abstract

The studies regarding soil bacterial community and correlation analysis of wild-simulated ginseng cultivation area are insufficient. The purpose of this study was to investigate the correlation between soil bacterial community and growth characteristics of wild-simulated ginseng for selection of suitable cultivation area. The bacterial community was investigated by high throughput sequencing technique (Illumina platform). The correlation coefficient between soil bacterial community and growth characteristics were analyzed using Spearman's rank correlation. The soil bacterial community from soil samples of 8 different wild-simulated ginseng cultivated area exhibited two distinct clusters, cluster 1 and cluster 2. The relative abundance of Proteobacteria (35.4%) and Alphaproteobacteria(24.4%) was observed to be highest in all soil samples. The lower soil pH and higher abundance of Acidobacteria resulted in increased growth of wild-simulated ginseng. Additionally, abundance of Acidobacteriia (class) and Koribacteraceae (family) demonstrated significant positive correlation with fresh weight of wild-simulated ginseng. The results of this study clearly state the correlation between growth characteristic and soil bacterial community of wild-simulated ginseng cultivation area, thereby offering effective insight into selection of suitable cultivation area of wild-simulated ginseng.

본 연구는 전국 임의의 산양삼 재배지를 선정하여 재배지 내의 토양 특성 및 토양세균군집을 분석하고, 토양 이화학적 특성, 토양세균군집 및 산양삼 생육특성 간의 상관관계를 구명하기 위하여 수행되었다. 토양세균군집 분석은 pyrosequencing analysis (Illumina platform)를 이용하였고, 토양세균군집과 생육특성 간의 상관관계는 Spearman's rank correlation을 이용하여 분석하였다. 8개 산양삼 재배지로부터 분리한 토양세균군집은 2개의 군집으로 군집화를 이루는 것을 확인하였다. 모든 토양샘플에서 Proteobacteria와 Alphaproteobacteria가 각각 35.4%, 24.4%로 가장 높은 상대적 빈도수를 보였다. 산양삼의 생육은 토양 pH가 낮고 Acidobacteria의 상대적 빈도수가 높은 토양에서 증가하였으며, Acidobacteriia (class)와 Koribacteraceae (family)의 상대적 빈도수는 산양삼의 생육과 유의적인 정의 상관관계를 보이는 것으로 나타났다. 본 연구 결과는 토양세균군집과 산양삼 생육 간의 상관관계를 구명하는 중요한 자료가 될 것으로 생각되고, 나아가 산양삼 재배 이전에 산양삼의 생육에 유용한 토양세균군집을 확인할 수 있다면 산양삼 재배적지를 선정하는데 도움을 줄 수 있을 것이다. 또한 토양이화학성과 더불어 임상 및 주변식생에 따른 토양세균군집과 산양삼 생육특성에 대한 상관관계 연구를 추가로 수행한다면 보다 명확한 정보를 대한 제공할 수 있을 것으로 사료된다.

Keywords

References

  1. Bartram AK, X Jiang, MDJ Lynch, AP Masella, GW Nicol, J Dushoff and JD Neufeld. 2014. Exploring links between pH and bacterial community composition in soil from the Craibstone Experimental Farm. FEMS Microbiol. Ecol. 87:403-415. https://doi.org/10.1111/1574-6941.12231
  2. Busby PE, C Soman, MR Wagner, ML Friesen, J Kremer, A Bennett, M Morsy, JA Eisen, JE Leach and JL Dangl. 2017. Research priorities for harnessing plant microbiomes in sustainable agriculture. PLOS Biol. 15:1-14.
  3. Cao H, R Chen, L Wand, L Jiang, F Yang, S Zheng, G Wang and X Lin. 2016. Soil pH, total phosphorus, climate and distance are the major factors influencing microbial activity at a regional spatial scale. Sci. Rep. 6:25815. https://doi.org/10.1038/srep25815
  4. Denance N, A Sanchez-Vallet, D Goffner and A Molina. 2013. Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front. Plant Sci. 4:155. https://doi.org/10.3389/fpls.2013.00155
  5. Dong L, J Xu, Y Li, H Fang, W Niu, X Li, Y Zhang, W Ding and S Chen. 2018. Manipulation of microbial community in the rhizosphere alleviates the replanting issues in Panax ginseng. Soil Biol. Biochem. 125:64-74. https://doi.org/10.1016/j.soilbio.2018.06.028
  6. Hartmann M, PA Nikalus, S Zimmermann, S Schmutz, J Kremer, K Abarenkov, P Luscher, F Widmer and B Frey. 2014. Resistance and resilience of the forest soil microbiome to loggingassociated compaction. ISME J. 8:226-244. https://doi.org/10.1038/ismej.2013.141
  7. Hatfield JL and JH Prueger. 2015. Temperature extremes: Effect on plant growth and development. Weather Clim. Extrem. 10:4-10. https://doi.org/10.1016/j.wace.2015.08.001
  8. Jeon KS, YR Um, CR Jung, HW Park and MJ Kim. 2018. Standard Cultivation Manual of Wild-Simulated Ginseng. National Institute of Forest Science, Seoul, Korea.
  9. Jeong JH, KS Koo, CH Lee and CS Kim. 2002. Physico-chemical properties of Korean forest soils by regions. J. Korean For. Soc. 91:694-700.
  10. Kim KY, S Samaddar, P Chatterjee, R Krishnammorthy, SY Jeon and TM Sa. 2019. Structural and functional responses of microbial community with respect to salinity levels in a coastal reclamation land. Appl. Soil Ecol. 137:96-105. https://doi.org/10.1016/j.apsoil.2019.02.011
  11. KOFPI. 2013. The cultivation of wild-simulated ginseng. pp. 14-34. In Wild -Simulated Ginseng and Cultural Environments. Korea Forestry Promotion Institute, Seoul, Korea.
  12. Kown SD, JH Kang, JH Yoon and HS Moon. 2011. An analysis on site, soil and cultivation characteristics of Korean mountain cultivated ginseng (Panax ginseng) filed. J. Agric. Life Sci. 45: 81-88.
  13. KSVS. 2014. Know-How of Characteristics Investigation of the Crops: Ginseng (Panax ginseng Meyer). Korea Seed and Variety Service, Gimcheon, Korea.
  14. Lee BJ and SH Eo. 2017. Soil bacterial community in red pine forest of Mt. Janggunbong, Bonghwa-gun, Gyeongbuk, Korea, using next generation sequencing. J. Korean For. Soc. 106: 121-129. https://doi.org/10.14578/JKFS.2017.106.2.121
  15. Lee DS. 2010. Weather characteristic and growth of a forest ginseng cultivation site. J. Korean For. Soc. 99:863-870.
  16. Lee DS. 2011. Theory and Practice of Growing Forest Cultivated Ginseng. Nexus Publish, Paju, Korea.
  17. Li H, D Ye, X Wang, ML Settles, J Wang, Z Hao, L Zhou, P Dong, Y Jiang and ZS Ma. 2014. Soil bacterial communities of different natural forest types in Northeast Chaina. Plant Soil 383: 203-216. https://doi.org/10.1007/s11104-014-2165-y
  18. Lipied J, C Doussan, A Nosalewicz and K Kondracka. 2013. Effect of drought and heat stresses on plant growth and yeild: a review. Int. Agrophys. 27:463-477. https://doi.org/10.2478/intag-2013-0017
  19. Nguyen NL, YJ Kim, VA Hoang, S Subramaniyam, JP Kang, CH Kang and DC Yang. 2016. Bacterial diversity and community structure in Korean ginseng field soil are shifted by cultivation time. PLOS ONE 11:e0155055. https://doi.org/10.1371/journal.pone.0155055
  20. Pandey P, V Irulappan, MV Bagacathiannan and M Senthil-Kumar. 2017. Impact of combined abiotic and biotic stresses on plant growth and avenues for crop improvement by exploiting physio-morphological traits. Front. Plant Sci. 8:537.
  21. Pettersson M. 2004. Factors Affecting the Rates of Change in Soil Bacterial Communities. Lund University, Sweden.
  22. Prasad R, S Kamal, PK Sharma, R Oelmueller and A Varma. 2013. Root endophyte Piriformospora indica DSM 11827 alters plants morphology, enhances biomass and antioxidant activity of medicinal plant Bacopa monniera. J. Basic Microbiol. 52: 1016-1024.
  23. Prasad R, M Kumar and A Varma. 2015. Role of PGPR in soil fertility and plant health. pp. 247-260. In Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plant (Egamberdieva et al. eds.). Springer, Switzerland.
  24. Ramegowda V and M Senthil-Kumar. 2015. The interactive effect of simultaneous biotic and abiotic stresses on plant: mechanistic understanding from drought and pathogen combination. J. Plant Physiol. 176:47-54. https://doi.org/10.1016/j.jplph.2014.11.008
  25. RDA. 2013. Analysis Manual of Comprehensive Examination Laboratory (Soil, Plant, Water and Liquid manure). Rural Development Administration, Suwon, Korea. pp. 31-53.
  26. Ren B, Y Hu, B Chen, Y Zhang, L Thiele, R Shi, M Liu and R Bu. 2018. Soil pH and plant diversity shape soil bacterial community structure in the active layer across the latitudinal gradient in continuous permafrost region of Northeastern China. Sci. Rep. 8:5619. https://doi.org/10.1038/s41598-018-24040-8
  27. Schloss PD. 2009. Ahigh-throughput DNA sequence aligner for microbial ecology studies. PLOS ONE 4:e8230. https://doi.org/10.1371/journal.pone.0008230
  28. Schloss PD, SL Westcott, T Ryabin, JR Hall, M Hartmann, EB Hollister, RA Lesniewski, BB Oakley, DH Parks, CJ Robinson, JW Sahl, B Stres, GG Thallinger, DJ van Horn and CF Weber. 2009. Introducing mothur: open source, platform-independent, community-supported software for describing and comparing microbial communities. Appl. Environ. Microbiol. 75:7537-7541. https://doi.org/10.1128/AEM.01541-09
  29. Selvakumar G, KY Kim, S Hu and TM Sa. 2014. Effect of salinity on plant and the role of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria in alleviation of salt stress. pp. 115-137. In Physiological Mechanism and Adaptation Strategies in Plant under Changing Environment (Ahmad P and MR Wani eds.). Springer, New York, USA.
  30. Sogin ML, HG Morrison, JA Huber, DM Welch, SM Huse, PR Neal, JM Arrieta and GJ Herndl. 2006. Microbial diversity in the deep sea and the underexplored "rare biosphere". PNAS 103:12115-12120. https://doi.org/10.1073/pnas.0605127103
  31. Song X, B Tao, J Guo, J Li and G Chen. 2018. Changes in the microbial community structure and soil chemical properties of vertisols under different cropping systems in Northern China. Front. Environ. Sci. 6:132. https://doi.org/10.3389/fenvs.2018.00132
  32. Sun H, Q Wang, N Liu, C Zhang, Z Liu and Y Zhang. 2017. Effects of different leaf litters on the physicochemical properties and bacterial communities in Panax ginseng-growing soil. Appl. Soil Ecol. 111:17-24. https://doi.org/10.1016/j.apsoil.2016.11.008
  33. Takaya SB, S Tipayno, KY Kim, P Subramanian and TM Sa. 2014. Rhizobactera: Restoration of heavy metal contaminated soil. pp. 297-323. In Physiological Mechanism and Adaptation Strategies in Plant under Changing Environment (Ahmad P and MR Wani eds.). Springer, New York, USA.
  34. Wang Q, H Sun, C Xu, L Ma, M Li, C Shao, Y Guan and N Liu. 2019. Analysis of rhizosphere bacterial and fungal communities associated with rusty root disease of Panax ginseng. Appl. Soil Ecol. 138:245-252. https://doi.org/10.1016/j.apsoil.2019.03.012
  35. Wisam ARA, AA Fadhal, HK Jamal and HH Wurood. 2018. Study physiology of roots growth for soybean by WinRhizo pro software with Vam3 genes. pp. 1-22. In Proceedings of the International Conference on Promotion of Scientific & Regional Cooperation on Food and Agricultural Sciences. Mashhad, Iran.
  36. Wu Y, J Zeng, Q Zhu, Z Zhang and X Lin. 2017. pH is the primary determinant of the bacterial community structure in agricultural soils impacted by polycyclic aromatic hydrocarbon pollution. Sci. Rep. 7:40093. https://doi.org/10.1038/srep40093
  37. Wu Z, Q Liu, Z Li, W Cheng, J Sun, Z Guo, Y Li, J Zhou, D Meng, H Li, P Lei and H Yin. 2018. Environmental factors shaping the diversity of bacterial communities that promote rice production. BMC Microbiol. 18:51. https://doi.org/10.1186/s12866-018-1174-z
  38. Xue L, H Ren, S Li, X Leng and X Yao. 2017. Soil bacterial community structure and co-occurrence pattern during vegetation restoration in karst rocky desertification area. Front. Microbiol. 8:2377. https://doi.org/10.3389/fmicb.2017.02377