Environmental Engineering Research

  • 제24권2호
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  • Pages.254-262
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  • 2019
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  • 1226-1025(pISSN)
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  • 2005-968X(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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Heterotrophic nitrification-aerobic denitrification potential of cyanide and thiocyanate degrading microbial communities under cyanogenic conditions

  • Mekuto, Lukhanyo (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Kim, Young Mo (School of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Ntwampe, Seteno K.O. (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Mewa-Ngongang, Maxwell (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Mudumbi, John Baptist N. (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Dlangamandla, Nkosikho (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Itoba-Tombo, Elie Fereche (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology) ;
  • Akinpelu, E.A. (Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology)
  • 투고 : 2018.04.13
  • 심사 : 2018.07.26
  • 발행 : 2019.12.27
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초록

The impact of free cyanide ($CN^-$) and thiocyanate ($SCN^-$) on the $CN^-$ (CDO) and $SCN^-$ degraders (TDO) to nitrify and denitrify aerobically was evaluated under alkaline conditions. The CDO's were able to nitrify under cyanogenic conditions, achieving $NH_4{^+}-N$ removal rates above 1.66 mg $NH_4{^+}-N.L^{-1}.h^{-1}$, except when $CN^-$ and $SCN^-$ loading was 15 mg $CN^-/L$ and 50 mg $SCN^-.L^{-1}$, respectively, which slightly inhibited nitrification. The TDO's were able to achieve a nitrification rate of 1.59 mg $NH_4{^+}-N.L^{-1}.h^{-1}$ in the absence of both $CN^-$ and $SCN^-$, while the presence of $CN^-$ and $SCN^-$ was inhibitory, with a nitrification rates of 1.14 mg $NH_4{^+}-N.L^{-1}.h^{-1}$. The CDO's and TDO's were able to denitrify aerobically, with the CDO's obtaining $NO_3{^-}-N$ removal rates above 0.67 mg $NO_3{^-}-N.L^{-1}.h^{-1}$, irrespective of the tested $CN^-$ and $SCN^-$ concentration range. Denitrification by the TDO's was inhibited by $CN^-$, achieving a removal rate of 0.46 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ and 0.22 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ when $CN^-$ concentration was 10 and 15 mg $CN^-.L^{-1}$, respectively. However, when the CDO's and TDO's were co-cultured, the nitrification and aerobic denitrification removal rates were 1.78 mg $NH_4{^+}-N.L^{-1}.h^{-1}$ and 0.63 mg $NO_3{^-}-N.L^{-1}.h^{-1}$ irrespective of $CN^-$ and $SCN^-$ concentrations.

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