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Characteristics of fruiting bodies formed upon monohybrid cross of Lentinula edodes strains

표고 단포자 교배에 따른 자실체 발생 특성

  • Ka, Kang-Hyeon (Special Forest Products Division, National Institute of Forest Science) ;
  • Ryoo, Rhim (Special Forest Products Division, National Institute of Forest Science) ;
  • Jang, Yeongseon (Special Forest Products Division, National Institute of Forest Science) ;
  • Park, Youngae (Special Forest Products Division, National Institute of Forest Science) ;
  • Jeong, Yeun Sug (Special Forest Products Division, National Institute of Forest Science) ;
  • Kang, Jae Jun (Special Forest Products Division, National Institute of Forest Science) ;
  • Heo, Guemsim (Special Forest Products Division, National Institute of Forest Science) ;
  • Jeon, Sung-Min (Special Forest Products Division, National Institute of Forest Science)
  • 가강현 (국립산림과학원 산림소득자원연구과) ;
  • 유림 (국립산림과학원 산림소득자원연구과) ;
  • 장영선 (국립산림과학원 산림소득자원연구과) ;
  • 박영애 (국립산림과학원 산림소득자원연구과) ;
  • 정연석 (국립산림과학원 산림소득자원연구과) ;
  • 강재준 (국립산림과학원 산림소득자원연구과) ;
  • 허금심 (국립산림과학원 산림소득자원연구과) ;
  • 전성민 (국립산림과학원 산림소득자원연구과)
  • Received : 2019.05.21
  • Accepted : 2019.06.21
  • Published : 2019.06.01

Abstract

Sawdust cultivation of Lentinula edodes has been increasing in Korea. Fourteen strains were used to develop the best varieties of L. edodes, and hybridization was carried out by monohybrid cross. The number of hybridized strains was 1,638 among 3,100 combinations. They were cultivated on sawdust medium, and fruiting bodies were formed in 364 strains. Among them, 65 strains were selected as superior candidate strains based on the shape and size of the fruiting bodies. Forty strains formed fruiting bodies without lamellae structure. The shape of the stipe was cylindrical (255 strains), thick to lower part (15 strains), and thick to upper part (94 strains). By the combinations of 2462 n1-10 and 3420 n1-10, 2462 n1, 2462 n2, 2462 n10, and 3420 n3 were selected as excellent monokaryotic strains. These strains were considered to be superior monokaryotic strains that could be used for hybrid breeding.

표고는 동아시아 지역에서 매우 중요한 식용버섯이다. 표고 우량품종을 개발하기 위해 14개 균주를 사용하였고, 단포자교잡을 통해 3,100개의 조합으로부터 1,638개의 교잡계통을 육성하였다. 교잡계통의 톱밥재배 시험결과 364개 계통의 버섯이 발생하였으며, 버섯의 모양과 크기를 기준으로 65개의 우량 후보계통을 최종 선발하였다. 364개 계통 중 주름이 없는 균주는 40개, 대의 모양이 원통형인 균주는 255개, 대 아래쪽이 뭉툭한 형태는 15개, 대 위쪽이 뭉툭한 형태는 94개로 관찰되었다. 10번의 조합을 가진 2462 n1-n10과 3420 n1-n10을 기준으로 했을 때, 65개의 우량한 후보 계통 중 5회 이상 동일 단포자가 포함된 것은 2462 n1, 2462 n2, 2462 n10, 3420 n3 이었다. 이들 단포자 균주들은 표고의 교잡계통을 만드는데 우수한 균주로 판단된다.

Keywords

References

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