BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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An alpha-lipoic acid-decursinol hybrid compound attenuates lipopolysaccharide-mediated inflammation in BV2 and RAW264.7 cells

  • Kwon, Mi-Youn (Department of Physiology and Biophysics, College of Medicine, Inha University) ;
  • Park, Jiwon (Department of Physiology and Biophysics, College of Medicine, Inha University) ;
  • Kim, Sang-Min (Department of Physiology and Biophysics, College of Medicine, Inha University) ;
  • Lee, Jooweon (Department of Physiology and Biophysics, College of Medicine, Inha University) ;
  • Cho, Hyeongjin (Department of Chemistry, Inha University) ;
  • Park, Jeong-Ho (Department of Chemical & Biological Engineering, Hanbat National University) ;
  • Han, Inn-Oc (Department of Physiology and Biophysics, College of Medicine, Inha University)
  • Received : 2019.05.20
  • Accepted : 2019.06.13
  • Published : 2019.08.31
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Abstract

In this study, the anti-inflammatory effects of ${\alpha}-lipoic$ acid (LA) and decursinol (Dec) hybrid compound LA-Dec were evaluated and compared with its prodrugs, LA and Dec. LA-Dec dose-dependently inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) generation in BV2 mouse microglial cells. On the other hand, no or mild inhibitory effect was shown by the Dec and LA, respectively. LA-Dec demonstrated dose-dependent protection from activation-induced cell death in BV2 cells. LA-Dec, but not LA or Dec individually, inhibited LPS-induced increased expressions of induced NO synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in a dose-dependent manner in both BV2 and mouse macrophage, RAW264.7 cells. Furthermore, LA-Dec inhibited LPS-induced expressions of iNOS, COX-2, interleukin-6, tumor necrosis $factor-{\alpha}$, and $interleukin-1{\beta}$ mRNA in BV2 cells, whereas the same concentration of LA or Dec was ineffective. Signaling studies demonstrated that LA-Dec inhibited LPS-activated signal transducer and activator of transcription 3 and protein kinase B activation, but not nuclear factor-kappa B or mitogen-activated protein kinase signaling. The data implicate LA-Dec hybrid compound as a potential therapeutic agent for inflammatory diseases of the peripheral and central nervous systems.

Keywords

References

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