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Differences in isolates of Tomato yellow leaf curl virus in tomato fields located in Daejeon and Chungcheongnam-do between 2017 and 2018

  • Oh, June-Pyo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Go-Woon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jungkyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Oh, Min-Hee (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Kang-Hee (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Jongseok (Department of Horticulture Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Domier, Leslie L. (United States Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champaign, Department of Crop Sciences) ;
  • Hammond, John (United States Department of Agriculture-Agricultural Research Service, United States National Arboretum, Floral and Nursery Plants Research Unit) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Received : 2019.03.19
  • Accepted : 2019.06.25
  • Published : 2019.09.01

Abstract

To follow up on a 2017 survey of tomato virus diseases, samples with virus-like symptoms were collected from the same areas (Buyeo-gun, Chungchungnam-Do and Daejeon, Korea) in 2018. While in 2017 mixed infections of Tomato mosaic virus with either Tomato yellow leaf curl virus (TYLCV) or Tomato chlorosis virus were detected, only TYLCV was detected in symptomatic samples in 2018. TYLCV amplicons of c.777 bp representing the coat protein (CP) coding region were cloned from the TYLCV positive samples, and the sequence data showed a 97.17% to 98.84% nucleotide and 98.45% to 99.22% amino acid identity with the 2017 Buyeo-gun isolate (MG787542), which had the highest amino acid (aa) sequence identity of up to 99.2% with four 2018 Buyeo-gun sequences (MK521830, MK521833, MK521834, and MK521835). The lowest aa sequence identity of 98.45% was found in a 2018 Daejeon isolate (MK521836); the distance between Buyeo-gun and Daejeon is about 45 km. Phylogenetic analysis indicated that the currently reported CP sequences are most closely related to Korean sequences from Masan (HM130912), Goseong (JN680149), Busan (GQ141873), Boseong (GU325634), and the 2017 isolate TYLCV-N (MG787543) in the 'Japan' cluster of TYLCV isolates and distinct from the 'China' cluster isolates from Nonsan (GU325632), Jeonju (HM130913) and Jeju (GU325633, HM130914). Our survey data from 2017 and 2018 suggest that TYLCV has become established in Korea and may be spread by whitefly vectors from weed reservoirs within the farm environment.

Keywords

References

  1. Cohen S, Harpaz I. 1964. Periodic rather than continual acquisition of a new tomato virus by its vector, the tobacco whitefly (Bemisia tabaci Gennadius). Entomologia Experimentalis et Applicata 7:155-166. https://doi.org/10.1111/j.1570-7458.1964.tb02435.x
  2. Choi GW, Kim B, Ju H, Cho S, Seo E, Kim J, Park J, Hammond J, Lim HS. 2018. Dual infections of Tomato mosaic virus (ToMV) and Tomato yellow leaf curl virus (TYLCV), or Tomato mosaic virus (ToMV) and Tomato chlorosis virus (ToCV), detected in tomato fields located in Chungcheongnam-do in 2017. Korean Journal of Agricultural Science 45:38-42. [In Korean] https://doi.org/10.7744/KJOAS.20180008
  3. Czosnek H, Laterrot H. 1997. A worldwide survey of tomato leaf curl viruses. Archives of Virology 142:1391-1406. https://doi.org/10.1007/s007050050168
  4. Fauquet CM, Sawyer S, Idris AM, Brown JK. 2005. Sequence analysis and classification of apparent recombinant begomoviruses infecting tomato in the Nile and Mediterranean Basins. Phytopathology 95:549-555. https://doi.org/10.1094/PHYTO-95-0549
  5. Idris AM, Brown JK. 2005. Evidence for interspecies-recombination for three begomoviral genomes associated with tomato leaf curl disease from central Sudan. Archives of Virology 150:1003-1012. https://doi.org/10.1007/s00705-004-0484-7
  6. Ioannou N, Kyriakou A, Hadjinicolis A. 1987. Host range and natural reservoirs of Tomato yellow leaf curl virus. Tech. Bull. Agricultural Research Institute, Ministry of Agriculture and Natural Resources Technical Bulletin 85:1-7.
  7. Jorda C, Font I, Martinez P, Juarez M, Ortega A, Lacasa A. 2001. Current status and new natural hosts of Tomato yellow leaf curl virus (TYLCV) in Spain. Plant Disease 85:445-445.
  8. Kil EJ, Byun HS, Kim S, Cho S, Cho S, Roh K, Lee KY, Choi HS, Kim CS, Lee S. 2015. Tomato yellow leaf curl virus can overwinter in Stellaria aquatica, a winter-hardy TYLCV-reservoir weed. Plant Disease 99:588-592. https://doi.org/10.1094/PDIS-04-14-0352-RE
  9. Kil EJ, Byun HS, Kim S, Kim J, Park J, Cho S, Yang DC, Lee KY, Choi HS, Kim JK, Lee S. 2014b. Sweet pepper confirmed as a reservoir host for Tomato yellow leaf curl virus by both agro-inoculation and whitefly-mediated inoculation. Archives of Virology 159:2387-2395. https://doi.org/10.1007/s00705-014-2072-9
  10. Kil EJ, Kim S, Lee YJ, Byun HS, Park J, Seo H, Kim CS, Shim JK, Lee JH, Kim JK, Lee KY, Choi HS, Lee S. 2016. Tomato yellow leaf curl virus (TYLCV-IL): A seed-transmissible geminivirus in tomatoes. Scientific Reports 6:19013 https://doi.org/10.1038/srep19013
  11. Kil EJ, Park J, Lee H, Kim J, Choi HS, Lee KY, Kim CS, Lee S. 2014a. Lamium amplexicaule (Lamiaceae): A weed reservoir for Tomato yellow leaf curl virus (TYLCV) in Korea. Archives of Virology 159:1305-1311. https://doi.org/10.1007/s00705-013-1913-2
  12. Kim MK, Kwak HR, Jeong SG, Ko SJ, Lee SH, Park JW, Kim KH, Choi HS, Cha BJ. 2007. First report on Tomato bushy stunt virus infecting tomato in Korea. The Plant Pathology Journal 23:143-150 https://doi.org/10.5423/PPJ.2007.23.3.143
  13. Kim SH, Oh S, Oh TK, Park JS, Kim SC, Kim YS, Hong JK, Sim SY, Park KS, Lee HG, Kim KJ, Choi CW. 2011. Genetic diversity of tomato-infecting Tomato yellow leaf curl virus (TYLCV) isolates in Korea. Virus Genes 42:117-127. https://doi.org/10.1007/s11262-010-0541-0
  14. Kirthi N, Maiya SP, Murthy MR, Savithri HS. 2002. Evidence for recombination among the Tomato leaf curl virus strains/species from Bangalore, India. Archives of Virology 147:255-272. https://doi.org/10.1007/s705-002-8318-8
  15. Lapidot M, Guenoune Gelbart D, Leibman D, Holdengreber V, Davidovitz M, Machbash Z, Klieman-Shoval S, Cohen S, Gal-On A. 2010. Pelargonium zonate spot virus is transmitted vertically via seed and pollen in tomato. Phytopathology 100:798-804. https://doi.org/10.1094/PHYTO-100-8-0798
  16. Lee H, Song W, Kwak HR, Kim JD, Park J, Auh CK, Kim DH, Lee KY, Lee S, Choi HS. 2010. Phylogenetic analysis and inflow route of Tomato yellow leaf curl virus (TYLCV) and Bemisia tabaci in Korea. Molecules and Cells 30:467-476. https://doi.org/10.1007/s10059-010-0143-7
  17. Moriones E, Navas Castillo J. 2000. Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Research 71:123-134. https://doi.org/10.1016/S0168-1702(00)00193-3
  18. Oh J, Lee HK, Park CY, Yeom YA, Min HG, Yang HJ, Jeong RD, Kim H, Moon JS, Lee SH. 2018. First report of Southern tomato virus in tomato (Solanum lycopersicum) in Korea. Plant Disease 102:1467-1468. https://doi.org/10.1094/PDIS-11-17-1746-PDN
  19. Ohnesorge B, Sharaf N, Allawi T. 1980. Population studies on the tobacco whitefly Bemisia tabaci Genn. (Homoptera; Aleyrodidae) during the winter season. Journal of Applied Entomology 90:226-232.
  20. Padidam M, Sawyer S, Fauquet CM. 1999. Possible emergence of new geminiviruses by frequent recombination. Virology 265:218-225. https://doi.org/10.1006/viro.1999.0056
  21. Papayiannis L, Katis N, Idris A, Brown J. 2011. Identification of weed hosts of Tomato yellow leaf curl virus in Cyprus. Plant Disease 95:120-125. https://doi.org/10.1094/PDIS-05-10-0346
  22. Pita JS, Fondong VN, Sangare A, Otim Nape GW, Ogwal S, Fauquet CM. 2001. Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda. Journal of General Virology 82:655-665. https://doi.org/10.1099/0022-1317-82-3-655
  23. Salati R, Nahkla MK, Rojas MR, Guzman P, Jaquez J, Maxwell DP, Gilbertson RL. 2002. Tomato yellow leaf curl virus in the Dominican Republic; Characterization of an infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts. Phytopathology 92:487-496. https://doi.org/10.1094/PHYTO.2002.92.5.487
  24. Sanz AI, Fraile A, Garcia Arenal F, Zhou X, Robinson DJ, Khalid S, Butt T, Harrison BD. 2000. Multiple infection, recombination and genome relationships among begomovirus isolates found in cotton and other plants in Pakistan. Journal of General Virology 81:1839-1849. https://doi.org/10.1099/0022-1317-81-7-1839
  25. Saunders K, Salim N, Mali VR, Malathi VG, Briddon R, Markham PG, Stanley J. 2002. Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: Evidence for acquisition of a DNA B component by a monopartite begomovirus. Virology 293:63-74. https://doi.org/10.1006/viro.2001.1251
  26. Sawangjit S, Chatchawankanphanich O, Chiemsombat P, Attathom T, Dale J, Attathom S. 2005. Possible recombination of tomato-infecting begomoviruses in Thailand. Journal of General Plant pathology 71:314-318. https://doi.org/10.1007/s10327-005-0206-3
  27. Zhou X, Perry JN, Woiwod IP, Bale JS, Clark SJ. 1997. Temperature change and complex dynamics. Oecologia 112:543-550. https://doi.org/10.1007/s004420050343