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Development of Extracellular Matrix (ECM) based Dermal Filler

세포외기질(ECM) 생체소재 기반 필러 개발 연구

  • Kim, Na Hyeon (Department of Biomedical Engineering, Pukyong National University) ;
  • Park, Sang-Hyug (Department of Biomedical Engineering, Pukyong National University)
  • 김나현 (부경대학교 대학원 의생명기계전기융합과정) ;
  • 박상혁 (부경대학교 대학원 의생명기계전기융합과정)
  • Received : 2019.08.14
  • Accepted : 2019.08.20
  • Published : 2019.08.31

Abstract

Numerous efforts are being made to develop an ideal dermal filler that should be bio-compatibility, non-immunogenicity, long-lasting and biodegradable without a toxic secretion. Biomaterials of dermal fillers are hyaluronic acid filler, calcium filler, PMMA filler and collagen filler depending on the ingredient. Although hyaluronic acid (HA) is most widely used, it has shortages such as short shelf life and low mechanical strength compare to extracellular matrix (ECM). The cartilage ECM composed of collagen type II, proteoglycans, glycosaminoglycans (GAGs) and in a minor part with glycoproteins. In this study, we developed a cartilage ECM injectable filler capable of improving biocompatibility and longevity compared with hyaluronic acid (HA) fillers. The ECM hydrogel was cross-linked by the reaction of N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) for mechanical enhancement. Prepared ECM filler was compared with cross-linked HA by butanediol diglycidyle ether (BDDE), which is the most widely used natural polymers for dermal filler. In the results, the articular cartilage ECM hydrogel has great potential as a dermal filler to improve the biophysical and biological performance.

Acknowledgement

Supported by : 부경대학교

References

  1. Gilchrest BA. Skin aging and photoaging: an overview. Journal of the American Academy of Dermatology. 1989;21(3):610-3. https://doi.org/10.1016/S0190-9622(89)70227-9
  2. Fisher GJ, Kang S, Varani J, Bata-Csorgo Z, Wan Y, Datta S, Voorhees JJ. Mechanisms of photoaging and chronological skin aging. Archives of dermatology. 2002;138(11):1462-70.
  3. Fisher GJ, Wang ZQ, Datta SC, Varani J, Kang S, Voorhees JJ. Pathophysiology of premature skin aging induced by ultraviolet light. New England Journal of Medicine. 1997;337(20):1419-29. https://doi.org/10.1056/NEJM199711133372003
  4. Helfrich YR, Sachs DL, Voorhees JJ. Overview of skin aging and photoaging. Dermatology nursing. 2008;20(3):177.
  5. Newton VL, Mcconnell JC, Hibbert SA, Graham HK, Watson RE. Skin aging: molecular pathology, dermal remodelling and the imaging revolution. G Ital Dermatol Venereol. 2015; 150(6):665-74.
  6. Cheng LY, Sun XM, Tang MY, Jin R, Cui WG, Zhang YG. An update review on recent skin fillers. Plast Aesthet Res. 2016;3:92-9. https://doi.org/10.20517/2347-9264.2015.124
  7. Beasley KL, Weiss MA, Weiss RA. Hyaluronic acid fillers: a comprehensive review. Facial Plastic Surgery. 2009;25(02):086-094. https://doi.org/10.1055/s-0029-1220647
  8. Fakhari A, Berkland C. Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment. Acta biomaterialia. 2013;9(7):7081-92. https://doi.org/10.1016/j.actbio.2013.03.005
  9. Leach JB, Schmidt CE. Characterization of protein release from photocrosslinkable hyaluronic acid-polyethylene glycol hydrogel tissue engineering scaffolds. Biomaterials. 2005; 26(2):125-35. https://doi.org/10.1016/j.biomaterials.2004.02.018
  10. Maleki A, Kjoniksen AL, Nystrom B. Characterization of the chemical degradation of hyaluronic acid during chemical gelation in the presence of different cross-linker agents. Carbohydrate research. 2007;342(18):2776-92. https://doi.org/10.1016/j.carres.2007.08.021
  11. Schante CE, Zuber G, Herlin C, Vandamme TF. Chemical modifications of hyaluronic acid for the synthesis of derivatives for a broad range of biomedical applications. Carbohydrate polymers. 2011;85(3):469-89. https://doi.org/10.1016/j.carbpol.2011.03.019
  12. Badylak SF, Freytes DO, Gilbert TW. Extracellular matrix as a biological scaffold material: structure and function. Acta biomaterialia. 2009;5(1):1-13. https://doi.org/10.1016/j.actbio.2008.09.013
  13. Theocharis AD, Skandalis SS, Gialeli C, Karamanos NK. Extracellular matrix structure. Advanced drug delivery reviews. 2016;97:4-27. https://doi.org/10.1016/j.addr.2015.11.001
  14. Carter DR, Beaupre GS, Wong M, Smith RL, Andriacchi TP, Schurman DJ. The mechanobiology of articular cartilage development and degeneration. Clinical Orthopaedics and Related Research. 2004;427:S69-S77. https://doi.org/10.1097/01.blo.0000144970.05107.7e
  15. Sophia Fox AJ, Bedi A, Rodeo SA. The basic science of articular cartilage: structure, composition, and function. Sports health. 2009;1(6):461-8. https://doi.org/10.1177/1941738109350438
  16. Hay ED. Cell biology of extracellular matrix ed. New York:Plenum Press; 1991. pp. 419-62.
  17. Tavsanli B, Okay O. Preparation and fracture process of high strength hyaluronic acid hydrogels cross-linked by ethylene glycol diglycidyl ether. Reactive and Functional Polymers. 2016;109:42-51. https://doi.org/10.1016/j.reactfunctpolym.2016.10.001
  18. Werb Z. ECM and cell surface proteolysis: regulating cellular ecology. Cell. 1997; 91(4):439-42. https://doi.org/10.1016/S0092-8674(00)80429-8
  19. Baek J, Fan Y, Jeong SH, Lee HY, Jung HD, Kim HE, Kim S, Jang TS. Facile strategy involving low-temperature chemical cross-linking to enhance the physical and biological properties of hyaluronic acid hydrogel. Carbohydrate polymers. 2018;202:545-53. https://doi.org/10.1016/j.carbpol.2018.09.014
  20. Wang MO, Etheridge JM, Thompson JA, Vorwald CE, Dean D, Fisher JP. Evaluation of the in vitro cytotoxicity of crosslinked biomaterials. Biomacromolecules. 2013;14(5):1321-9. https://doi.org/10.1021/bm301962f