대한약침학회지 (Journal of Pharmacopuncture)

  • 제22권3호
  • /
  • Pages.140-146
  • /
  • 2019
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

Biochemical, Hematological Effects and Complications of Pseudosynanceia Melanostigma Envenoming

  • Babaie, Mahdi (Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University) ;
  • Zolfagharian, Hossein (Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO)) ;
  • Zolfaghari, Mohammad (Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University) ;
  • Jamili, Shahla (Department of Marine Biology, Faculty of Marine Sciences and Technologies, Science and Research Branch, Islamic Azad University)
  • 투고 : 2017.04.29
  • 심사 : 2019.09.03
  • 발행 : 2019.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: Venomous fishes have different pharmacological effects and are useful. Among the venomous fish, stonefishes; especially Pseudosynanceia melanostigma has various pharmacological effects on the nervous, muscular and cardiovascular system of humans. In this study, toxicological characteristics, some blood effects, pharmacological and enzymatic properties of Pseudosynanceia melanostigma venom was investigated. Methods: Crude venom purified by using gel filtration chromatography and the molecular weights of the venom and its fractions were estimated. The approximate LD values of this venom were determinedand the effects of LD50 dose on the blood of rabbits were studied. Hemolytic and Hemorrhagic activity of the venom sample was determined. In this case coagulation tests were performed. Results: The LD50 of the Pseudosynanceia melanostigma crude venom was also determined to be $194.54{\mu}g/mouse$. The effect of two doses of LD50 showed a non-significant differences decrease in RBCs and MCV. In other cases, the results showed significant differences in WBC, Plt, Hb, MCH, MCHC and HCT; also it's showed a significant decrease. WBC count showed a significant increase with two doses of LD50 groups. The prothrombin time and partial prothrombin time were increased after venom treatment. As well as bleeding and clotting time were increased. According to the results, a minimum dose for Haemorrhagic effect $40{\mu}g$ was obtained. Conclusion: Venom of Pseudosynanceia melanostigma has inhibitory effect on platelet aggregation that can be used to design and develop of anticoagulant drugs.

키워드

참고문헌

  1. Smith WL, Wheeler WC. Venom evolution widespread in fishes: A road map for the bio prospecting of piscine venoms. J Hered. 2006;97(3):206-17. https://doi.org/10.1093/jhered/esj034
  2. Saggiomo SL, Zelenka C, Seymour J. Relationship between food and venom production in the estuarine stonefish Synanceia horrida. Toxicon. 2017;125:19-23. https://doi.org/10.1016/j.toxicon.2016.11.250
  3. Tay TK, Chan HZ, Ahmad TS, Teh KK, Low TH, Wahab NA. Stonefish envenomation of hand with impending compartment syndrome. J Occup Med Toxicol. 2016;11:23-29. https://doi.org/10.1186/s12995-016-0112-y
  4. Ouanov G, Malo M, Stinnakre J, Kreger AS, Molgo J. Trachinilysin, a neurosecretory protein isolated from stonefish (Synanceia trachynis) venom, from nonselective pores in the membrane of NG108-cel. J Biol Chem and Mol Biol. 2002;277(42):39119-27. https://doi.org/10.1074/jbc.M203433200
  5. Breton P, Delamanche I, Buee J, Goudey-Perriere F, Perriere C. Evidence for a neurotoxic activity in crude venom of the stonefish (Synanceia verrucosa). J Nat Toxins. 2002;11(4):305-13.
  6. Jafari M, Mohebbi GH, Vazirizadeh A, Nabipour I. Medical management in stonefish envenomation in Bushehr port. ISMJ. 2014;17(3):496-505.
  7. Baek JH, Lee SH. Identification and characterization of venom proteins of two solitary wasps, Eumenes pomiformis and Orancistrocerus drewseni. Toxicon. 2010;56(4):554-562. https://doi.org/10.1016/j.toxicon.2010.05.014
  8. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Falin phenol reagent. J Biol Chem. 1951;193(1):265-75. https://doi.org/10.1016/S0021-9258(19)52451-6
  9. Babaie M, Zolfagharian H, Salmanizadeh H, Mirakabadi AZ, Alizadeh H. Isolation and partial purification of anti-coagulant fractions from the venom of the Iranian snake Echis carinatus. Acta Biochim Pol. 2013;60(1):17-20.
  10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-85. https://doi.org/10.1038/227680a0
  11. Meier J, Theakston RGD. Approximate LD50 determination of snake venoms using eight to ten experimental animals. Toxicon. 1986;24(4):395-401. https://doi.org/10.1016/0041-0101(86)90199-6
  12. Chinedu E, Arome D, Solomon Ameh F. A New Method for Determining Acute Toxicity in Animal Models. Toxicol Int. 2013;20(3):224-6. https://doi.org/10.4103/0971-6580.121674
  13. Abd El-Aal A, Abd El-Ghffar EA, Soliman MM. Isolation and characterization of a myotoxic fraction from cerastes Vipera snake venom. J Toxins. 2017;4(1):1-9.
  14. Kondo H, Kondo S, Ikezawa H, Murata R, Ohsaka A. Studies on the quantitative method for determination of hemorrhagic activity of Habu snake venom. Jpn J Med Sci Biol. 1960;13:43- 51. https://doi.org/10.7883/yoken1952.13.43
  15. Sami JA, Khalid GA, Baida RA. Effect of crude venom of Echis carinatus sochureki snake on hematological parameters of male and female rats. World J Pharm Sci. 2015;3(8):1687-92.
  16. Mielke CH. Measurement of the bleeding time: international committee communications.Thromb Haemostas. 1984;52:210-1. https://doi.org/10.1055/s-0038-1661174
  17. Khoo HE, Yuen R, Poh CH, Tan CH. Biological Activities of Synancda horrida (stonefish) Venom. Nat Toxins. 1992;1(1):54-60. https://doi.org/10.1002/nt.2620010111
  18. Rizzo F, Papasouliotis K, Crawford E, Dodkin S, Cue S. Measurement of prothrombin time (PT) and activated partial thromboplastin time (APTT) on canine citrated plasma samples following different storage conditions. Res Vet Sci. 2008;85(1):166-70. https://doi.org/10.1016/j.rvsc.2007.10.001
  19. Zolfagharian H, Mohajeri M, Babaie M. Honey Bee Venom (Apis mellifera) contains anti coagulation factors and increases the blood-clotting time. J Pharmacopuncture. 2015;18(4):7-11. https://doi.org/10.3831/KPI.2015.18.031
  20. Church JE, Hodgson WC. Adrenergic and cholinergic activity contributes to the cardiovascular effects of lionfish (Pterois volitans) venom. Toxicon. 2002;40(6):787-96. https://doi.org/10.1016/S0041-0101(01)00285-9
  21. Tu AT, Hendon RR. Characterisation of lizard venom hyaluronidase and evidence for its action as a spreading factor. Comp Biochem Physiol B. 1983;6(2):377-83.
  22. Saunders PR, Tokes L. Purification and properties of the lethal fraction of the venom of the stonefish, Synanceja horrida. Biochem Biophys Acta. 1961;30(52):527-32. https://doi.org/10.1016/0006-3002(61)90411-5
  23. Garnier P, Goudeyperriere F, Breton P, Dewulf C, Petek F, Perriere C. Enzymatic properties of the stonefish (Synanceja verrucosa Bloch and Schneider, 1801) venom and purification of a lethal, hypotensive and cytolytic factor. Toxicon. 1995;33(2):143-55. https://doi.org/10.1016/0041-0101(94)00151-W
  24. Lopes-Ferreira M, Barbaro KC, Cardoso DF, Moura Da-Silva AM, Mota L. Thalassophryne nattereri fish venom: biological and biochemical characterization and serum neutralization of its toxic activities. Toxicon. 1998;36(2):405-10. https://doi.org/10.1016/S0041-0101(97)00115-3
  25. Weiner S. Observations on the venom of the stonefish (Synanceja trachynis). Med J Aust. 1959;46(19):620-7. https://doi.org/10.5694/j.1326-5377.1959.tb59324.x
  26. Chhatwal L, Dreyer F. Biological properties of crude venom extract from the greater weever fish Trachinusdraco. Toxicon. 1992;30(1):77-85. https://doi.org/10.1016/0041-0101(92)90503-W
  27. Kamiguti AS, Cardoso JL, Theakston RD, Sano-Martins IS, Hutton RA, Rugman FP, et al. Coagulopathy and haemorrhage in human victims of Bothrops jararaca envenoming in Brazil. Toxicon. 1991;29(8):961-72. https://doi.org/10.1016/0041-0101(91)90079-7
  28. Kamiguti AS, Rugman FP, Theakston RD, Franca FO, Ishii H, Hay CR. The role of venom haemorrhagin in spontaneous bleeding in Bothrops jararaca envenoming. Thromb Haemost. 1992;67(4):484-8. https://doi.org/10.1055/s-0038-1648475
  29. Shiomi K, Hosaka M, Fujita S, Yamanaka H, Kituchi T. Venoms from six species of marine fish: lethal and haemolytic activities and their neutralization by commercial stonefish antivenom. Mar Biol. 1989;103(3):285-9. https://doi.org/10.1007/BF00397261