Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Influence of blood serum, follicular fluid and gonadotropin on in vitro maturation for goat oocytes

혈청과 난포액 및 성선자극호르몬 첨가가 염소 난자의 체외성숙에 미치는 영향

  • Lee, Sang-Hoon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Jeon, Dayeon (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Jinwook (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Soo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Seungchang (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Kwan-Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 이상훈 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 전다연 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이진욱 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 이성수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김승창 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김찬란 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김관우 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2019.07.19
  • Accepted : 2019.09.06
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the effects of goat blood serum (gBS), goat follicular fluid (gFF) and gonadotropin (FSH) on the in vitro maturation, fertilization and development of Korean native black goat oocytes. Our results indicate that the gBS combined with FSH treated group showed significantly higher maturation rate than the other groups. Furthermore, blastocyst formation rate was significantly increased in all treated groups, and gBS and gFF combined with FSH treated groups were higher than other groups. However, gene expression levels of BMP15 and GDF9 in COC, both oocyte maturation related genes, remained unaffected after 24 h maturation. The results of the present study indicate that supplementation of the maturation medium with gBS, gFF and FSH is efficacious in improving the in vitro maturation, fertilization and development of Korea native black goat oocytes.

본 연구는 염소 난자의 체외배양 시 혈청과 호르몬 첨가의 효과를 알아보기 위하여 체외성숙 효율, 유전자발현 및 체외수정 후 배발달율을 조사하였다. 본 실험에서는 기본 체외성숙 배양액에 10% gBS, 10% gFF를 첨가하여 혈청유무에 따른 효과와 FSH를 조합하여 체외성숙 효율을 조사한 결과, 처리구 모두 대조구보다 유의적으로 높은 성숙률을 보였다. 특히 gBS와 FSH를 조합하였을 때 처리구 중에서도 77.8%로 유의적으로 가장 높은 체외성숙 결과를 보였다. 또한, 각 처리구로부터 얻은 성숙 난자를 이용하여 체외수정 배발달 효율을 조사한 결과 모두 유의적으로 향상되었으며 특히 gBS와 gFF에 FSH를 혼합 처리한 두 그룹이 우수한 결과를 보였다. 난자의 성숙과 관련된 BMP15와 GDF9의 mRNA 발현량을 비교한 결과, 처리구간 특이점이나 유의적 차이가 나타나지 않았다. 본 연구의 결과 염소 난자의 체외성숙에 관한 추가적인 실험이 필요할 것으로 생각되나, gBS, gFF 및 FSH 첨가가 성숙 효율 향상에 도움이 됨을 알 수 있었다.

Keywords

References

  1. L. L. Mamuad, S. H. Kim, S. S. Lee, K. K. Cho, C. O. Jeon, S. S. Lee, "Characterization, metabolites and gas formation of fumarate reducing bacteria isolated from Korean native goat (Capra hircus coreanae )". Journal of Microbiology, Vol.50, No.6, pp.925-931, 2012. DOI: https://doi.org/10.1007/s12275-012-2497-3
  2. B. Y. Jung, M. G. Seo, S. H. Lee, J. W. Byun, J. K. Oem, D. Kwak, "Molecular and serologic detection of Coxiella burnetii in native Korean goats (Capra hircus coreanae )", Veterinary Microbiology, Vol.173, No.1-2, pp.152-155, 2014. DOI: https://doi.org/10.1016/j.vetmic.2014.06.029
  3. S. W. Kim, S. B. Park, M. J. Kim, D. H. Kim, D. G. Yim, "Effects of different levels of concentrate in the diet on physicochemical traits of Korean native black goat meats", Korean J Food Sci Anim Resour, Vol.34, No.4, pp.457-463, 2014. DOI: https://doi.org/10.5851/kosfa.2014.34.4.457
  4. A. M. M. T Reza, S. Shiwani, N. K. SinghEmail author, J. D. Lohakare, S. J. Lee, D. K. Jeong, J. Y. Han, D. Rengaraj, B. W. Lee,"Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage", In Vitro Cellular and Developmental Biology. Animal , Vol.50, No.3, pp. 194-206, 2014. DOI: https://doi.org/10.1007/s11626-013-9690-5
  5. S. Y. Jang, E. K. Kim, J. H. Park, M. R. Oh, Y. J. Tang, Y. L. Ding, H. J. Seong, W. H. Kim, Y. S. Yun, S. H. Moon, "Effects of physically effective neutral detergent fiber content on dry matter intake, digestibility, and chewing activity in Korean native goats (Capra hircus coreanae ) fed with total mixed ration", Asian-Australasian Journal of Animal Sciences, Vol.30, No.10, pp.1405-1409, 2017. DOI: https://doi.org/10.5713/ajas.16.0868
  6. A. Bakhsh, I. Ismail, Y. H. Hwang, J. G. Lee, S. T. Joo, "Comparison of blood loss and meat quality characteristics in Korean black goat subjected to head-only electrical stunning or without stunning", Korean J Food Sci Anim Resour, Vol.38, No.6, pp.1286-1293, 2018. DOI: https://doi.org/10.5851/kosfa.2018.e64
  7. M. A. Sirard, H. M. Florman, M. L. Leibfried-Rutledge, F. L. Barnes, M. L. Sims, N. L. First, "Timing for nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes", Biol. Reprod., Vol.40, No.6, pp.1257-1263, 1989. DOI: https://doi.org/10.1095/biolreprod40.6.1257
  8. N. Kaivo-oja, L. A. Jeffery, O. Ritvos, D. G. Motter, "Smad signalling in the ovary", Reprod Biol Endocrinol. Vol.4, No.21, pp.1-13, 2006. DOI: https://doi.org/10.1186/1477-7827-4-21
  9. D. J. Trombly, T. K. Woodruff1, K. E. Mayo, "Roles for Transforming Growth Factor Beta Superfamily Proteins in Early Folliculogenesis", Semin Reprod Med. Vol.27, No.1, pp.14-23, 2009. DOI: https://doi.org/10.1055/s-0028-1108006
  10. S. A. Pangas, "Regulation of the ovarian reserve by members of the transforming growth factor beta family", Mol Reprod Dev. Vol.79, pp.666-679, 2012. DOI: https://doi.org/10.1002/mrd.22076
  11. F. Otsuka, K. McTavish, S.Shimasaki, "Integral Role of GDF-9 and BMP-15 in Ovarian Function", Mol Reprod Dev. Vol.78, pp.9-21, 2011. DOI: https://doi.org/10.1002/mrd.21265
  12. P. Lonergan, T. Fair, "Maturation of Oocytes in vitro ", Annu Rev Anim Biosci. Vol.4, pp.255-68, 2016. DOI: https://doi.org/10.1146/annurev-animal-022114-1108 22
  13. J. S. Lopes, A. Canha-Gouveia, E. París-Oller, P. Coy, "Supplementation of bovine follicular fluid during in vitro maturation increases oocyte cumulus expansion, blastocyst developmental kinetics, and blastocyst cell number", Theriogenology. Vol.126, Vol.1, pp.222-229, 2019. DOI:https://doi.org/10.1016/j.theriogenology.2018.12.010
  14. B. K. Redel, L. D. Spate, R. S. Prather, "In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos", Methods Mol Biol. Vol.2006, pp.93-103, 2019. DOI: https://doi.org/10.1007/978-1-4939-9566-0_6
  15. A. J. Watson, P. Sousa, A. Caveney, L. C. Barcroft, D. Natale, J. Urquhart, M. E. Westhusin, "Impact of bovine oocyte maturation media on oocyte transcript levels, blastocyst development, cell number, and apoptosis", Bio. Reprod., Vol.62, Vol.2, pp.355-364. 2000. DOI: https://doi.org/10.1095/biolreprod62.2.355
  16. N. A. R. Sa, L. A. Vieira, A. C. A. Ferreira, J. Cadenas, J. B. Bruno, C. Maside, F. G. C. Sousa, F. W. S. Cibin, B. G. Alves, A. P. R. Rodrigues, J. H. Leal-Cardoso, E. L. Gastal, J. R. Figueiredo, "Anethole Supplementation During Oocyte Maturation Improves In Vitro Production of Bovine Embryos", Reprod Sci. Vol.26, 1933719119831783, 2019. DOI: https://doi.org/10.1177/1933719119831783
  17. M. V. O. Santos, L. E. Nascimento, É. A. Praxedes, A. A. Borges, A. R. Silva, L. M. Bertini, A. F. Pereira, "Syzygium aromaticum essential oil supplementation during in vitro bovine oocyte maturation improves parthenogenetic embryonic development", Theriogenology. Vol.128, No.1, pp.74-80, 2019. DOI: https://doi.org/10.1016/j.theriogenology.2019.01.031
  18. Z. Cao, D. Gao, X. Tong, T. Xu, D. Zhang, Y. Wang, Y. Liu, Y. Li, Y. Zhang, Y, Pu, "Melatonin improves developmental competence of oocyte-granulosa cell complexes from porcine preantral follicles", Theriogenology. Vol.133, No.15, pp.133:149-158, 2019. DOI: https://doi.org/10.1016/j.theriogenology.2019.05.003
  19. J. D. Yoon, S. U. Hwang, M. Kim, Y. Jeon, S. H. Hyun, "Growth differentiation factor 8 regulates SMAD2/3 signaling and improves oocyte quality during porcine oocyte maturation in vitro ", Biol Reprod. Vol.101, No.1, pp.63-75, 2019. DOI: https://doi.org/10.1093/biolre/ioz066
  20. D. K. Gardner, "Mammalian embryo culture in the absence of serum or somatic cell support", Cell Ciol Int., Vol.18, No.12, pp.1163-1179, 1994. DOI: https://doi.org/10.1006/cbir.1994.1043
  21. K. P. McNatty, D. M. Smith, A. Markris, R. osathanonch, K. J. Ryan, "The microenvironment of the human antral follicle: interrelationships among the steroid levels in antral fluid, the population of granulosa cells, and the status of the oocyte in vivo and in vitro ", J. Clin. Endocrinol. Metab., Vol.49, pp.851-860, 1979. https://doi.org/10.1210/jcem-49-6-851
  22. KP McNatty and RS Sawers, "Relationship between the endocrine enviroment within the Graafin follicle and the subsequent rate of progesterone secretory human granulosa cells in vitro ", J. Endocrinol. Vol.66, pp.391-400, 1975. https://doi.org/10.1677/joe.0.0660391
  23. M. L. Leibfried-Rutledge, E. S. Critser, N. L. First, "Effects of fetal calf serum and bovine serum albumin on in vitro maturation and fertilization of bovine and hamster cumulus-oocyte complexes", Biol Reprod. Vol.35, No.4, pp.850-857, 1986. https://doi.org/10.1095/biolreprod35.4.850
  24. M. Del Collado, N. Z. Saraiva, F. L. Lopes, R. C. Gaspar, L. C. Padilha, R. R. Costa, G. F. Rossi, R. Vantini, J. M. Garcia, "Influence of bovine serum albumin and fetal bovine serum supplementation during in vitro maturation on lipid and mitochondrial behaviour in oocytes and lipid accumulation in bovine embryos", Reprod Fertil Dev. Vol.28, No.11, pp.1721-1732, 2015. DOI: https://doi.org/10.1071/RD15067
  25. B. Avery, L. Strobech, T. Jacobsen, I. B. Bogh, T. Greve, "In vitro maturation of bovine cumulus-oocyte complexes in undiluted follicular fluid: effect on nuclear maturation, pronucleus formation and embryo development", Theriogenology. Vol.59, No.3-4, pp. 987-999, 2003. DOI: https://doi.org/10.1016/S0093-691X(02)01139-1
  26. G. Puri, S. S. Chaudhary, V. K. Singh, A. K. Sharma, "Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis ) oocytes in vitro ", Vet World. Vol.8, No.2, pp.143-146, 2015. DOI: https://doi.org/10.14202/vetworld.2015.143-146
  27. Y. S. Zheng, M. A. Sirard, "The effect of sera, bovine serum albumin and follicular cells on in vitro maturation and fertilization of porcine oocytes", Theriogenology. Vol.37, No.4, pp.779-790. 1992. DOI: https://doi.org/10.1016/0093-691X(92)90041-O
  28. Y. Ducolomb, H. Gonzalez-Marquez, R. Fierro, I. Jimenez, E. Casas, D. Flores, E. Bonilla, Z. Salazar, M. Betancourt, "Effect of porcine follicular fluid proteins and peptides on oocyte maturation and their subsequent effect on in vitro fertilization", Theriogenology. Vol.79, No.6, pp.896-904. 2013. DOI: https://doi.org/10.1016/j.theriogenology.2013.01.024
  29. Y. H. Choi, E. M. Carnevale, G. E. Seidel, E. L. Squires, "Effects of gonadotropins on bovine oocytes matured in TCM-199", Theriogenology. Vol.56, No.4, pp.661-670, 2001. DOI: https://doi.org/10.1016/S0093-691X(01)00597-0
  30. A, Ali, M. A. Sirard, "Effect of the absence or presence of various protein supplements on further development of bovine oocytes during in vitro maturation", Biol. Reprod., Vol.66, No.4, pp.901-905, 2002. DOI: https://doi.org/10.1095/biolreprod66.4.901
  31. S. Kito, B. D. Bavister, "Male pronuclear formation and early embryonic development of hamster oocytes matured in vitro with gonadotropins, amino acid and cysteamine", J. Reprod. Fertil., Vol.110, No.1, pp.35-46. 1991. DOI: https://doi.org/10.1530/jrf.0.1100035
  32. M. A. Sirard, "Temporary inhibition of meiosis resumption in vitro by adenylate cyclase stimulation in immature bovine oocytes", Theriogenology. Vol.33, No.4, pp.757-767. 1990. DOI: https://doi.org/10.1016/0093-691X(90)90811-7