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Senecio iscoensis Hieron. 추출물의 Propionibacterium acnes에 의한 염증반응 억제효과

Suppressive effect of Senecio iscoensis Hieron. extract in Propionibacterium acnes-induced inflammatory signaling pathway

  • 신진학 (강원대학교 분자생명과학과) ;
  • 이은혜 (강원대학교 분자생명과학과) ;
  • 김선숙 (강원대학교 분자생명과학과) ;
  • 이동근 (한국생명공학연구원) ;
  • 노진경 (생물경제연구소 에콰도르) ;
  • 서수련 (강원대학교 분자생명과학과)
  • Shin, Jin Hak (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Lee, Eun Hye (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Kim, Seon Sook (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University) ;
  • Yi, Dong-Keun (International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Roh, Jin Kyung (Instituto de BioEconomia, El Batan) ;
  • Seo, Su Ryeon (Department of Molecular Bioscience, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University)
  • 투고 : 2019.06.10
  • 심사 : 2019.06.23
  • 발행 : 2019.09.30

초록

Propionibacterium acnes (P. acnes)는 모낭과 모공 속에 존재하며 세포 찌꺼기, 피지 및 주변 피부 조직의 대사 부산물을 에너지와 영양소로 사용한다. 과도한 피지생성과 모낭의 막힘으로 피지 생성이 증가하면 P. acnes의 증식 및 성장을 유발할 수 있다. 모낭에 있는 P. acnes의 급속한 성장은 염증을 일으킬 수 있는 세포 손상, 신진 대사 부산물을 생성한다. 본 연구는 S. iscoensis Hieron. (S. iscoensis) 추출물이 P. acnes에 의한 염증반응을 조절할 가능성이 있는지 확인하고, 그 신호 전달 기전을 밝히고자 하였다. S. iscoensis 추출물은 마우스 대식세포주인 Raw 264.7에서 P. acne에 의해 유도되는 IL-$1{\beta}$, TNF-${\alpha}$, iNOS와 같은 염증성 사이토카인의 발현을 매우 효과적으로 억제하였다. 이러한, 염증성 사이토카인의 발현 억제는 NF-${\kappa}B$와 NF-AT와 같은 전사 조절 인자의 활성화 저해를 통해 일어남을 확인하였다. 그러나, MAPK 신호 전달 기전과는 상관이 없음을 확인하였다. 이 연구는 S. iscoensis 추출물이 여드름의 치료제로 사용될 가능성이 있음을 최초로 제안한다.

Propionibacterium acnes (P. acnes) lives in the hair follicles and pores, and it uses cell debris, sebum and metabolic byproducts of surrounding skin tissues as energy and nutrients. Increased production of sebum due to sebaceous hyperplasia or blockage of the follicle can cause growth and proliferation of P. acnes. The rapid growth of P. acnes in follicles produces cell damage, metabolic byproducts and bacterial chips, which can cause inflammation. In this study, we examined the possibility of Senecio iscoensis Hieron. (S. iscoensis) extract to regulate P. acnes-induced inflammatory signaling pathways. We observed that S. iscoensis extract effectively inhibited P. acnes-induced pro-inflammatory cytokine expressions such as IL-$1{\beta}$, TNF-${\alpha}$, and iNOS in mouse macrophage cell line Raw 264.7. The inhibitory effect of S. iscoensis in pro-inflammatory cytokine levels was accompanied by the inhibition of the transcription factors NF-${\kappa}B$ and NF-AT. However, S. iscoensis did not alter the P. acnes-induced MAPK signaling pathways. This study first suggests the potential of using S. iscoensis extract as an alternative agent for the treatment of acne.

키워드

참고문헌

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