Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and characterization of acid-resistanct and halophilic bacteria using cultivation technique in Jeju island

배양기법을 활용한 제주도내 내산 및 호염성 미생물의 분리 및 특성 분석

  • Han, Bit (Department of Biology, Jeju National University) ;
  • Kim, Minji (Department of Biology, Jeju National University) ;
  • Ryu, Dajung (Department of Biology, Jeju National University) ;
  • Lee, Ki-Eun (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Lee, Byoung-Hee (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Lee, Eun-Young (Exhibition & Education Division, National Institute of Biological Resources) ;
  • Park, Soo-Je (Department of Biology, Jeju National University)
  • 한빛 (제주대학교 생물학과) ;
  • 김민지 (제주대학교 생물학과) ;
  • 류다정 (제주대학교 생물학과) ;
  • 이기은 (국립생물자원관 미생물자원과) ;
  • 이병희 (국립생물자원관 미생물자원과) ;
  • 이은영 (국립생물자원관 전시교육과) ;
  • 박수제 (제주대학교 생물학과)
  • Received : 2019.04.23
  • Accepted : 2019.08.21
  • Published : 2019.09.30
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Abstract

In this study, we isolated about 70 bacterial strains from terrestrial and marine environments in Jeju island, and finally, total 21 strains were obtained based on the 16S ribosomal RNA gene sequence analysis. These isolated strains were classified into 16 genera of 5 classes and were identified as an unrecorded species in the Republic of Korea. As a result of the substrate utilization and capability for polymer degradation, the physiological phenotypes for acid resistance and halophilic bacteria were observed to be distinct from each other, except for some acid resistance strains. This study might provide basic information on utilization for indigenous microorganisms.

본 연구에서는 제주 지역의 토양 및 해양 환경으로부터 약 70주의 미생물들을 분리하였으며, 16S ribosomal RNA 유전자 분석을 통하여 최종 21종의 미생물을 발굴하였다. 이들 미생물들은 5 강(Class) 16 속(Genus)에 속하며, 모두 국내 미기록종으로 확인되었다. 분리된 미생물의 기질 특이성 및 고분자 물질 분해능을 바탕으로 내산성과 호염성 미생물들의 생리활성 표현형은 서로 구별되는 것으로 관찰되었다. 본 연구결과는, 국내 미생물 자원활용에 기초적 정보를 제공할 것으로 기대된다.

Keywords

References

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