Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Complete genome sequencing of Pseudomonas parafulva PpaJBCS1880, a biocontrol and plant growth promoting agent

식물 병 방제 및 생육촉진 효과를 나타내는 Pseudomonas parafulva PpaJBCS1880균주의 유전체 염기서열

  • Dutta, Swarnalee (Division of Biotechnology, Chonbuk National University) ;
  • Wabyona, Alex (Division of Biotechnology, Chonbuk National University) ;
  • Kakembo, David (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Yong Hoon (Division of Biotechnology, Chonbuk National University)
  • Received : 2019.06.27
  • Accepted : 2019.08.28
  • Published : 2019.09.30
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Abstract

Pseudomonas parafulva PpaJBCS1880 (PpaJBCS1880) isolated from rice seeds showed strong antagonistic activity against bacterial plant pathogens by producing lipopeptide. Furthermore, the strain controlled the incidence of bacterial pustule in soybean plants and promoted the growth of rice plants. Here we present complete genome sequence of PpaJBCS1880. The genome comprises of 5,208,480 bp with GC content of 63.4%, which includes 4,487 predicted protein-coding genes, 19 rRNAs, and 74 tRNAs. Genome analysis revealed genes encoding antimicrobial secondary metabolites such as lipopeptide, pyoverdine, phenazine, and hydrogen cyanide, which are known to play essential roles in biocontrol of plant diseases.

벼 종자에서 분리한 Pseudomonas parafulva PpaJBCS1880 (PpaJBCS1880) 균주는 lipopeptide를 분비하여 식물의 세균 병원균에 대해 강력한 항균력을 나타냈다. 또한, PpaJBCS1880는 콩불마름병의 발생을 억제하였을 뿐만 아니라, 벼의 생육을 촉진하였다. 이에 따라, 본 연구에서는 PpaJBCS1880 균주의 전체염기서열을 해독하고 분석하였는데, 총 염기서열은 5,208,480 bp였고, GC 함량은 63.4%였다. 염색체는 4,487개의 단백질을 암호화하였고, 19개의 rRNA와 74개의 tRNA로 구성되어 있었다. 유전체의 분석을 통해 2차 대사산물인 lipopeptide, pyoverdine, phenazine 및 hydrogen cyanide 등을 생산하는 것을 확인하였는데, 이들 대사산물에 의해 항균력, 생물방제 및 생육촉진 효과를 나타내는 것으로 판단된다.

Keywords

References

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