DOI QR코드

DOI QR Code

토양 방선균인 Gordonia sp. MMS17-SY073 균주의 유전체 분석

Complete genome sequence of Gordonia sp. MMS17-SY073, a soil actinobacterium

  • 김영석 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 김승범 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Kim, Yeong Seok (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Seung Bum (Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University)
  • 투고 : 2019.08.14
  • 심사 : 2019.09.11
  • 발행 : 2019.09.30

초록

섬 해안가 토양에서 방선균주 Gordonia sp. MMS17-SY073를 분리하여 유전체 분석을 실시하였고, 그 결과 5,962,176 염기쌍 및 67.4%의 G + C 함량으로 이루어진 유전체 정보를 확보하였다. 유전정보 분석 결과 총 5,201개 단백질 지정 유전자, 6개 rRNA 유전자 및 45개 tRNA 유전자를 확인하였다. MMS17-SY073 균주는 16S rRNA 유전자를 이용한 분석 결과 분류학적으로 Gordonia soli의 표준균주와 가장 가까웠으며 그 유사도는 98.5%로 나타났다. MMS17-SY073 균주는 non-ribosomal peptide synthetase 유형을 비롯한 다수의 이차대사산물 생합성 유전자를 보유하고 있는 것으로 나타났다.

An actinobacterial strain designated Gordonia sp. MMS17-SY073 (=KCTC 49257) was isolated from a coastal soil of an island, and its complete genome was analyzed. A single contig consisting of 5,962,176 bp with the G + C content of 67.4% was obtained, and the annotation resulted in 5,201 protein-coding genes, 6 rRNA genes and 45 tRNA genes. Strain MMS17-SY073 was closest to the type strain of Gordonia soli based on the 16S rRNA gene sequence comparison, sharing 98.5% sequence similarity. A number of biosynthetic gene clusters for secondary metabolites, non-ribosomal peptide synthetase types in particular, could be identified from the genome.

키워드

참고문헌

  1. Arenskotter M, Daniel B, and Alexander S. 2004. Biology of the metabolically diverse genus Gordonia. Appl. Environ. Microbiol. 70, 3195-3204. https://doi.org/10.1128/AEM.70.6.3195-3204.2004
  2. Franzetti A, Caredda P, Ruggeri C, Colla PL, Tamburini E, Papacchini M, and Bestetti G. 2009. Potential applications of surface active compounds by Gordonia sp. strain BS29 in soil remediation technologies. Chemosphere 75, 801-807. https://doi.org/10.1016/j.chemosphere.2008.12.052
  3. Kim KK, Lee KC, Klenk HP, Oh HM, and Lee JS. 2009. Gordonia kroppenstedtii sp. nov., a phenol-degrading actinomycete isolated from a polluted stream. Int. J. Syst. Evol. Microbiol. 59, 1992-1996. https://doi.org/10.1099/ijs.0.005322-0
  4. Linos A, Steinbuchel A, Sproer C, and Kroppenstedt RM. 1999. Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre. Int. J. Syst. Bacteriol. 49, 1785-1791. https://doi.org/10.1099/00207713-49-4-1785
  5. Park S, Kang SJ, Kim W, and Yoon JH. 2009. Gordonia hankookensis sp. nov., isolated from soil. Int. J. Syst. Evol. Microbiol. 59, 3172-3175. https://doi.org/10.1099/ijs.0.011585-0
  6. Shen FT, Goodfellow M, Jones AL, Chen YP, Arun AB, Lai WA, and Young CC. 2006. Gordonia soli sp. nov., a novel actinomycete isolated from soil. Int. J. Syst. Evol. Microbiol. 56, 2597-2601. https://doi.org/10.1099/ijs.0.64492-0
  7. Torsten S. 2014. Prokka: rapid prokaryotic genome annotation, Bioinformatics 30, 2068-2069. https://doi.org/10.1093/bioinformatics/btu153
  8. Tsukamura M. 1971. Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J. Gen. Microbiol. 68, 15-26. https://doi.org/10.1099/00221287-68-1-15
  9. Xue Y, Sun X, Zhou P, Liu R, Liang F, and Ma Y. 2003. Gordonia paraffinivorans sp. nov., a hydrocarbon-degrading actinomycete isolated from an oil-producing well. Int. J. Syst. Evol. Microbiol. 53, 1643-1646. https://doi.org/10.1099/ijs.0.02605-0