DOI QR코드

DOI QR Code

야생화로부터 분리한 Bacillus halotolerans F41-3 균주의 전체 게놈서열

Complete genome sequence of Bacillus halotolerans F41-3 isolated from wild flower in Korea

  • 허준 (국립농업과학원 농업미생물과) ;
  • 김수진 (국립농업과학원 농업미생물과) ;
  • 김정선 (국립농업과학원 농업미생물과) ;
  • 홍승범 (국립농업과학원 농업미생물과) ;
  • 권순우 (국립농업과학원 농업미생물과)
  • Heo, Jun (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Kim, Soo-Jin (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Kim, Jeong-Seon (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Hong, Seung-Beom (Agricultural Microbiology Division, National Institute of Agricultural Sciences) ;
  • Kwon, Soon-Wo (Agricultural Microbiology Division, National Institute of Agricultural Sciences)
  • 투고 : 2019.09.09
  • 심사 : 2019.09.16
  • 발행 : 2019.09.30

초록

많은 Bacillus 속 균주들이 다양한 분야에서 이용할 수 있는 항균 활성을 가지고 있는 것으로 알려져 있다. 여드름을 유발하는 인자 중 하나인 Propionibacterium acne를 억제하기 위해 P. acnes에 대해 높은 항균활성을 가지는 Bacillus halotolerans F41-3 균주를 선발하였다. 우리는 B. halotolerans F41-3 균주에 대한 유전체 분석을 하였고 유전적 특성을 분석하였다. 이 유전체는 4,144,458 bp의 크기로 G + C 함량은 43.76%, 유전자의 수는 4,145개, 단백질 암호화 유전자는 3,686개로 구성되어 있다. 유전체 가운데 bacteriocin 중 하나인 subtilosin에 연관된 유전자 클러스터와 subtilosin 합성에 관여하는 nickel 수송에 연관된 유전자 클러스터가 포함되어 있는 것을 확인하였다. 두 클러스터가 P. acnes를 저해하는데 영향을 줄 것으로 보인다.

A number of Bacillus strains are known to have antimicrobial activity useful in various fields. In order to prevent Propionibacterium acnes, which is one of the factors of acne, we selected Bacillus halotolerans F41-3 which have high antimicrobial activities against P. acnes. We conducted complete genome sequencing of B. halotolerans F41-3 and analyzed genomic characteristics. This genome size is 4,144,458 bp with a G + C content of 43.76%, 4,145 total genes and 3,686 protein coding genes. Among the genes, we found that gene clulster of subtilosin, a kind of bacteriocin, synthesis and gene cluster of nickel transportation. Both of them may influence inhibition of P. acnes.

키워드

참고문헌

  1. Babasaki K, Takao T, Shimonishi Y, and Kurahashi K. 1985. Subtilosin A, a new antibiotic peptide produced by Bacillus subtilis 168: isolation, structural analysis, and biogenesis. J. Biochem. 98, 585-603. https://doi.org/10.1093/oxfordjournals.jbchem.a135315
  2. Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, et al. 2013. Nonhybrid, finished microbial genome assemblies from longread SMRT sequencing data. Nat. Methods 10, 563-569. https://doi.org/10.1038/nmeth.2474
  3. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR, da Costa MS, Rooney AP, Yi H, Xu XW, Meyer SD, et al. 2018. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int. J. Syst. Evol. Microbiol. 68, 461-466. https://doi.org/10.1099/ijsem.0.002516
  4. Kawulka KE, Sprules T, Diaper CM, Whittal RM, McKay RT, Mercier P, Zuber P, and Vederas JC. 2004. Structure of subtilosin A, a cyclic antimicrobial peptide from Bacillus subtilis with unusual sulfur to ${\alpha}$-carbon cross-links: Formation and reduction of ${\alpha}$-thio-${\alpha}$-amino acid derivatives. J. Biochem. 98, 585-603.
  5. Lee DS and Song HG. 2018. Antibacterial activity of isolated bacteria against Propionibacterium acnes causing acne vulgaris. Korean J. Microbiol. 54, 272-279. https://doi.org/10.7845/kjm.2018.8048
  6. Sumi CD, Yang BW, Yeo IC, and Hahm YT. 2015. Antimicrobial peptides of the genus Bacillus: a new era for antibiotics. Can. J. Microbiol. 61, 93-103. https://doi.org/10.1139/cjm-2014-0613
  7. Tatusova T, DiCuccio M, Badretdin A, Chetvernin V, Nawrocki EP, Zaslavsky L, Lomsadze A, Pruitt KD, Borodovsky M, and Ostell J. 2016. NCBI prokaryotic genome annotation pipeline. Nucleic Acids Res. 44, 6614-6624. https://doi.org/10.1093/nar/gkw569
  8. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, and Chun J. 2017, Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int. J. Syst. Evol. Microbiol. 67, 1613-1617. https://doi.org/10.1099/ijsem.0.001755