The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Acrophialophora ellipsoidea, an Undescribed Species Isolated from Soil in Korea

  • Ayim, Benjamin Yaw (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kim, Young-Tae (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Das, Kallol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2019.06.17
  • Accepted : 2019.09.05
  • Published : 2019.09.30
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Abstract

A designated fungal isolate, KNU-US-1802E was isolated from the soil in Uiseong, Korea. To identify characteristics of the isolate, it was cultured on PDA media for 6 days at $35^{\circ}C$. Colonies on PDA are flat, light gray, dense, with entire margins; reverse dark gray to black, with white margins. Aerial mycelia were smooth-walled, hyaline and 40~42 mm diameter after 6 days at $35^{\circ}C$. Conidia were hyaline, one-celled, ellipsoidal to fusiform, forming long chains with average length ${\times}$ width of $5.0{\pm}0.3{\times}2.9{\pm}0.2{\mu}m$. Molecular analysis indicates that the internal transcribed spacer (ITS) region and partial beta-tubulin (tub2) gene sequence showed 100% and 99% similarities, respectively with Acrophialophora ellipsoidea CGMCC 3.15255 collected from China. Phylogenetic analysis by the neighbor-joining (NJ) method shows that the KNU-US-1802E was clustered with A. ellipsoidea CGMCC 3.15255 in a phylogenetic tree constructed using the concatenated sequences of ITS region and tub2 gene sequences with a high bootstrap value. Based on these findings, the isolate KNU-US-1802E was identified as Acrophialophora ellipsoidea, and this is the first report of this isolate in Korea.

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References

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