The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Organic Acid Addition on Fruiting Productivity and Primordium Formation in Grifola frondosa

유기산을 이용한 pH 조절 처리가 잎새버섯 자실체 발이 및 생산성에 미치는 영향

  • Kim, Jeong-Han (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services) ;
  • Jang, Myoung-Jun (Department of Plant Resources, Kongju National University)
  • 김정한 (경기도농업기술원 버섯연구소) ;
  • 장명준 (공주대학교 산업과학대학 식물자원학과)
  • Received : 2019.07.31
  • Accepted : 2019.09.24
  • Published : 2019.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to develop a useful method for substrate pH adjustment in bottle cultivation of Grifola frondosa. Four types of organic acid solution-citric acid, malic acid, tartaric acid, and succinic acid-were added at 0.5% to 3.0% (w/v) into the substrate. The pH after the organic acid treatments ranged from 3.8 to 4.8 for the mycelial growth of G. frondosa after sterilization. Supplementation with succinic acid at 0.5% resulted in a shorter cultivation period and higher consistent yields compared to other organic acid treatments. These results indicate that the technique we developed is beneficial for higher production of G. frondosa using bottle cultivation.

잎새버섯 안정생산을 위하여 배지의 pH를 적정하게 조절할 수 있는 방법을 개발하고자 유기산을 이용한 pH 조절 및 적정 첨가농도를 구명하기 위하여 수행하였다. 혼합배지에 4종의 유기산(구연산, 호박산, 사과산, 주석산)를 5수준(0.5, 1, 1.5, 2, 2.5, 3%)으로 처리하여 균사생장정도를 측정한 결과 유기산 0.5% 수준에서 균사생장이 빨랐으며, 그 중에서도 호박산이 가장 우수하였다. 유기산의 첨가농도가 높을수록 혼합배지의 pH는 낮아지는 경향으로 배양기간 경과에 따라 대조구의 pH는 서서히 낮아지는 경향을 보인 반면 유기산 처리구는 유지되는 경향이었다. 발이율은 0.5%의 농도에서 호박산이 95%, 구연산 92.5%, 사과산과 주석산 80%로 대조구의 60%보다 우수하였다. 그러나 유기산의 농도가 1% 이상에서는 발이가 지연되거나 억제되는 경향을 보여주었다. 배양기간은 유기산 0.5% 처리구가 30~34일로 대조구의 35일보다 빠른 것으로 나타났으며, 전체 재배기간은 0.5% 호박산이 52일, 0.5%의 구연산과 사과산이 54일, 0.5%의 주석산이 52일로 대조구의 59일보다 빠른 것으로 나타났다. 병당 수량성은 0.5%의 호박산과 구연산 첨가 시 각각 121 g, 107 g으로 가장 우수하였고 대조구의 87.5 g에 비해 21~37% 증수되었다.

Keywords

References

  1. Mark, M. Maitake extracts and their therapeutic potential - A review. Altern Med Rev 2001;6:48-60.
  2. Wu MJ, Cheng TL, Cheng SY, Lian TW, Wang L, Chiou SY. Immunomodulatory properties of Grifola frondosa in submerged culture. J Agric Food Chem 2006;54:2906-14. https://doi.org/10.1021/jf052893q
  3. Kodama N, Murata Y, Asakawa A, Inui A, Hayashi M, Sakai N, Nanba H. Maitake D-fraction enhances antitumor effects and reduces immunosuppression by mitomycin-C in tumor-bearing mice. Nutrition 2005;21:624-9. https://doi.org/10.1016/j.nut.2004.09.021
  4. Nanba H, Kodama N, Schar D, Turner D. Effects of maitake (Grifola frondosa) glucan in HIVinfected patients. Mycoscience 2000;41:293-5. https://doi.org/10.1007/BF02463941
  5. Fukushima M, Ohashi T, Fujiwara Y, Sonoyama K, Nakano M. Cholesterol-lowering effects of maitake (Grifola frondosa) fiber, shiitake (Lentinus edodes) fiber, and enokitake (Flammulina velutipes) fiber in rats. Exp Biol Med 2001;226:758-65. https://doi.org/10.1177/153537020222600808
  6. Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res Int 2002;35:519-26. https://doi.org/10.1016/S0963-9969(01)00150-8
  7. Shen Q, Royse DS. Effects of nutrient supplements on biological efficiency, quality and crop cycle time of maitake (Grifola frondosa). Appl Microbiol Biotechnol 2001;57:74-8. https://doi.org/10.1007/s002530100748
  8. Jeon DH, Lee YH, Choi JI, Gwon HM, Chi JH, Hong HJ, Jang GY. Characteristics of a new Grifola frondosa cultivar “Daebak” with stable pinheading and high yield. J Mushrooms 2018;16:203-7.
  9. Cheong JC, Hong IP, Jang KY, Park JS. Culture condition and inoculum volume of liquid spawn on the bottled cultivation of Agarocybe cylindracea. Kor J Mycol 2003;31:94-7. https://doi.org/10.4489/KJM.2003.31.2.094
  10. Hong JS. Studies on the physio-chemical properties and the cultivation of oyster mushroom (Pleurotus ostreatus). Appl Biol Chem 1978;21:150-84.
  11. Kang MS, Kang TS, Kang AS, Shon HR, Sung JM. Studies on mycelial growth and artificial cultivation of Pleurotus eryngii. Kor J Mycol 2000;28:73-80.
  12. Chi JH, Kim JH, Won SY, Seo GS, Ju YC. Optimal condition of mycelial growth of Grifola frondosa. Kor J Mycol 2007;35:76-80. https://doi.org/10.4489/KJM.2007.35.2.076
  13. Shim JO, Son SG, Yoon SO, Lee YS, Lee TS, Lee SS, Lee KD, Lee MW. The optimal factors for the mycelial growth of Sparassis crispa. Kor J Mycol 1998;26:39-46.
  14. Kim JH, Choi JI, Chi JH, Won SY, Seo GS, Ju YC. Investigation on favorable substrate formulation for bag cultivation of Grifola frondosa. Kor J Mycol 2008;36:26-30. https://doi.org/10.4489/KJM.2008.36.1.026
  15. Chi JH, Kim JH, Won SY, Seo GS, Ju YC. Studies on favorable light condition for artificial cultivation of Grifola frondosa. Kor J Mycol 2008;36:31-5. https://doi.org/10.4489/KJM.2008.36.1.031