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Two Unreported Glomeromycota Fungi Isolated from Rhizospheres of Smilax china

청미래덩굴의 근권에서 분리된 2종의 Glomeromycota 미기록종

  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Ka, Kang-Hyeon (Special Forest Products Division, National Institute of Forest Science) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 박혁 (한국교원대학교 생물교육과) ;
  • 가강현 (국립산림과학원 산림소득자원연구과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2019.08.20
  • Accepted : 2019.09.25
  • Published : 2019.09.30

Abstract

We isolated fungal spores belonging to the phylum Glomeromycota from the rhizospheres of Smilax china, cultured in a greenhouse. We identified the isolated spores using sequence analysis of 18S partial rDNA region, internal transcribed spacer and 28S rDNA regions. We confirmed 2 unreported spores of Glomeromycota fungal species, Diversispora eburnea and Paraglomus laccatum. Here, we described the morphological characteristics and results of phylogenetic analysis of the confirmed species.

청미래덩굴의 근권 토양을 온실에서 배양하여 Glomeromycota에 속하는 균류의 포자를 분리하였다. 분리된 포자는 18S partial rDNA 영역의 DNA 염기서열 분석과, 더 정확한 동정을 위해 추가적으로 ITS 영역과 28S rDNA 영역까지 포함하는 $Kr{\ddot{u}}ger$ fragment 지역의 염기서열을 분석하여 동정하였다. 동정 과정에서 2종의 국내 미기록종 Glomeromycota 균류의 포자를 확인하였으며, 확인된 종은 Diversispora eburnea, Paraglomus laccatum이다. 확인된 미기록종 포자의 형태적 특성 및 계통적 분석의 결과에 대해 기술하였다.

Keywords

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