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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Deficiency of calpain-6 inhibits primary ciliogenesis

  • Kim, Bo Hye (Department of Biological Sciences, Sookmyung Women's University) ;
  • Kim, Do Yeon (Department of Biological Sciences, Sookmyung Women's University) ;
  • Oh, Sumin (Department of Biological Sciences, Sookmyung Women's University) ;
  • Ko, Je Yeong (Department of Biological Sciences, Sookmyung Women's University) ;
  • Rah, Gyuyeong (Department of Biological Sciences, Sookmyung Women's University) ;
  • Yoo, Kyung Hyun (Department of Biological Sciences, Sookmyung Women's University) ;
  • Park, Jong Hoon (Department of Biological Sciences, Sookmyung Women's University)
  • Received : 2019.07.17
  • Accepted : 2019.08.31
  • Published : 2019.10.31
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Abstract

The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of ${\alpha}-tubulin$ acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia.

Keywords

References

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