Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Study on the Cell Adhesion of Breast Cancer Cells using Nano/Micro Patterning PDMS

나노/마이크로 패턴 PDMS를 이용한 유방암 세포의 부착에 관한 연구

  • Kwak, Do Hoon (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Kim, Woo Cheol (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Jin, Hee Won (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Yun, Wan Su (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Park, Sanghyo (Department of Biomedical Engineering, College of Health Sciences, Yonsei University) ;
  • Key, Jaehong (Department of Biomedical Engineering, College of Health Sciences, Yonsei University)
  • 곽도훈 (연세대학교 보건과학대학 의공학부) ;
  • 김우철 (연세대학교 보건과학대학 의공학부) ;
  • 진희원 (연세대학교 보건과학대학 의공학부) ;
  • 윤완수 (연세대학교 보건과학대학 의공학부) ;
  • 박상효 (연세대학교 보건과학대학 의공학부) ;
  • 기재홍 (연세대학교 보건과학대학 의공학부)
  • Received : 2019.05.21
  • Accepted : 2019.10.04
  • Published : 2019.10.31
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Abstract

Cancer cells are different from normal cells in terms of life cycle, behavior, and growth patterns. Cancer cells can migrate freely in the body through blood vessels and lymph nodes. The cancer cells easily interact with various substrates including extracellular matrix and vessels and they can differentiate in the new environment. However, it is not well known about the adhesion preference of cancer cells on the substrate and the mechanism of their interaction. In this study, we prepared the nano-, micro-patterned substrates using E-beam lithography techniques. MCF-7 cells were tested on the substrates to find out their adhesion preference. The substrates were made by polydimethylsiloxane (PDMS) with specific patterns including pillars with a diameter of 500 nm, 700 nm, $3{\mu}m$ and $5{\mu}m$. MCF-7 cells were seeded on the substrates and incubated for 24 hours. As a result, this study clearly demonstrated that the MCF-7 cells preferred 700 nm patterning.

Keywords

References

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