Applied Chemistry for Engineering (공업화학)

The Korean Society of Industrial and Engineering Chemistry (한국공업화학회)
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Evaluation for Interactive Toxic Effects of Binary Heavy Metals on Bacterial Growth and Phosphorus Removal under Co-Culture Condition of Alcaligenes sp. and Pseudomonas sp.

Alcaligenes sp.와 Pseudomonas sp.의 공동배양 조건에서 박테리아 생장 및 인 제거에 미치는 두 종 중금속의 상호적인 독성효과 평가

  • Kim, Deok-Hyun (National Institute of Environmental Research) ;
  • Park, Sang-Wook (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Kim, Deok-Won (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Park, Ji-Su (EHS Part, Doosan Corporation Electro-Materials) ;
  • Oh, Eun-Ji (Water and Land Research Group/Division for Natural Environment, Korea Environment Institute) ;
  • Yoo, Jin (Department of Environmental and Biological Chemistry, Chungbuk National University) ;
  • Chung, Keun-Yook (Department of Environmental and Biological Chemistry, Chungbuk National University)
  • 김덕현 (국립환경과학원 토양지하수연구과) ;
  • 박상욱 (충북대학교 환경생명화학과) ;
  • 김덕원 (충북대학교 환경생명화학과) ;
  • 박지수 (두산전자 EHS팀) ;
  • 오은지 (한국환경정책평가연구원 자연환경연구실) ;
  • 유진 (충북대학교 환경생명화학과) ;
  • 정근욱 (충북대학교 환경생명화학과)
  • Received : 2020.09.01
  • Accepted : 2020.10.15
  • Published : 2020.12.10
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Abstract

This study was initiated to quantitatively evaluate the inhibitory effects of five heavy metals (Cd, Cu, Zn, Pb, Ni) on bacterial growth and phosphorus removal in the binary culture of Alcaligenes sp. plus Pseudomonas sp. IC50 values of Alcaligenes sp. plus Pseudomonas sp. for Cd, Cu, Zn, Pb, and Ni were 0.75, 10.93, 7.08, 13.30, and 15.78 mg/L, respectively. For the binary treatments of heavy metals, IC50 was the lowest in the treatment of Cd + Cu, whereas, it was the highest in the Ni + Pb treatment. The EC50 values for Cd, Cu, Zn, Pb, and Ni were 0.54, 11.08, 6.14, 9.33, and 13.81 mg/L, respectively. For the binary treatments of heavy metals, EC50 was the lowest in the Cd + Zn, whereas, the highest in the Zn + Ni. Based on both IC50 and EC50 values for the binary culture of bacteria with the binary mixtures of heavy metals, the most interactive effect was found to be antagonistic, though the only synergistic effect was found in Cu + Ni treatment. Therefore, our results can provide basic data on the toxic effects of heavy metals on the bacterial growth and phosphorus removal in the wastewater treatment process.

본 연구는 Alcaligenes sp.와 Pseudomonas sp. 균주를 2종 혼합 배양 시, 혼합균주의 생장 및 인 제거에 5종의 중금속(Cd, Cu, Zn, Pb, Ni)이 미치는 저해효과를 정량적으로 평가하고자 수행되었다. 단일 중금속 처리 시 중금속별 Alcaligenes sp.와 Pseudomonas sp. 균주의 IC50는 Cd 0.75 mg/L, Cu 10.93 mg/L, Zn 7.08 mg/L, Pb 13.3 mg/L, Ni 15.78 mg/L 인 것으로 나타났다. 중금속 2종 처리 시 IC50는 Cd + Cu 처리구에서 가장 낮은 농도를 보였고, Ni + Pb 처리구에서 가장 높은 농도를 보였다. 단일 중금속 처리 시 중금속별 EC50 값은 Cd 0.54 mg/L, Cu 11.08 mg/L, Zn 6.14 mg/L, Pb 9.33 mg/L 및 Ni 13.81 mg/L이었다. 2종의 중금속 혼합 처리구별 EC50은 Cd + Zn 처리구에서 가장 낮은 농도, Zn + Ni 처리구에서 가장 높은 농도를 보였다. 2종 혼합 균주에 대한 2종 중금속 처리 결과 도출된 IC50 및 EC50 값을 기반 중금속 별 상호작용 평가 결과, 대부분 길항작용을 보이는 것으로 나타났으나, Cu + Ni 처리구에서는 상승작용을 보이는 것이 확인되었다. 따라서 본 연구 결과는 미생물을 이용한 오폐수 정화 처리 시 미생물의 성장 및 인 제거에 미치는 중금속의 독성으로 인한 저해효과에 대한 기초 자료를 제공할 수 있을 것으로 판단된다.

Keywords

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