DOI QR코드

DOI QR Code

Physicochemical Properties and Antioxidant Activity of Extract from Astragalus membranaceus Bunge Leaf Fermented with Lactic Acid Bacteria

유산균으로 발효한 황기 잎 추출물의 이화학적 특성 및 항산화 활성

  • Song, Bit Na (Fermentated Processing Food Science Division, NIAS, RDA) ;
  • Lee, Da Bin (Fermentated Processing Food Science Division, NIAS, RDA) ;
  • Lee, Sung Hyun (Functional Food & Nutrition Division, NIAS, RDA) ;
  • Park, Bo Ram (Fermentated Processing Food Science Division, NIAS, RDA) ;
  • Choi, Ji Ho (Fermentated Processing Food Science Division, NIAS, RDA) ;
  • Kim, Yong Suk (Department of Food Science and Technology, Chonbuk National University) ;
  • Park, Shin Young (Fermentated Processing Food Science Division, NIAS, RDA)
  • 송빛나 (농촌진흥청 국립농업과학원 농식품자원부 발효가공식품과) ;
  • 이다빈 (농촌진흥청 국립농업과학원 농식품자원부 발효가공식품과) ;
  • 이성현 (농촌진흥청 국립농업과학원 기능성식품과) ;
  • 박보람 (농촌진흥청 국립농업과학원 농식품자원부 발효가공식품과) ;
  • 최지호 (농촌진흥청 국립농업과학원 농식품자원부 발효가공식품과) ;
  • 김용석 (전북대학교 식품공학과) ;
  • 박신영 (농촌진흥청 국립농업과학원 농식품자원부 발효가공식품과)
  • Received : 2020.09.29
  • Accepted : 2020.11.09
  • Published : 2020.12.30

Abstract

Background: This study aimed to investigate the quality characteristics of Astragalus membranaceus Bunge leaf (AMBL) fermented with lactic acid bacteria and the applicability of its biologically active compounds. Methods and Results: An assessment of physicochemical properties such as pH, total acidity, free sugars, and isoflavonoid (calycosin-7-o-β-d-glucoside, ononin, calycosin, and formononetin) was conducted. Furthermore, the levels of antioxidant compounds, including polyphenols and flavonoids, and radical scavenging activities of the extracts using 2,2-Diphenyl-1-picryl-hydrazyl-hydrate and 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were investigated. The calycosin content in the water extract of AMBL fermented with Leuconostoc mesenteroides increased by approximately twice as much as the control. Conclusions: These results indicate that L. mesenteroides can be used to improve biological activity through fermentation, and that AMBL can be used as a functional materials and edible resource in industrial areas.

Keywords

References

  1. Ahn HY, Park KR, Kim YR, Cha JY and Cho YS. (2013). Chemical characteristics in fermented cordycepin-enriched Cordyceps militaris. Journal of the Korean of Life Science. 23:1032-1040. https://doi.org/10.5352/JLS.2013.23.8.1032
  2. Bae EA, Han MJ, Kim EJ and Kim DH. (2004). Transformation of gingeng saponins to ginsenoside Rh2 by acids and human intestinal bacteria and biological activities of their transformants. Archives of Pharmacal Research. 27:61-67. https://doi.org/10.1007/BF02980048
  3. Bedir E, Calis I, Piacente S, Pizza C and Khan IA. (2000). A new flavonol glycoside from the aerial parts of Astragalus vulneraria. Chemical and Pharmaceutical Bulletin. 48:1994-1995. https://doi.org/10.1248/cpb.48.1994
  4. Blois MS. (1958). Antioxidant determination by the use of a stable free radical. Nature. 26:1199-1200. https://doi.org/10.1038/1811199a0
  5. Chien H, Huang H and Chou C. (2006). Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. Journal of Food Microbiology. 23:772-778. https://doi.org/10.1016/j.fm.2006.01.002
  6. Cho SI, Kim HW and Lee GJ. (2006). Biological activites of extracts of fermented Camellia japonica leaf and flower. Korean Journal of Herbology. 21:55-62.
  7. Choi YB, Woo JG and Noh WS. (1999). Hydrolysis of βglucosidic bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Korean of Journal Food science Technology. 31:189-195.
  8. Du XG, Z Hao B, Li JY, Cao XH, Diao MK, Feong HB, Chen XB, Cheon ZY and Zeong XY. (2012). Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturaion and suppressing Treg frequency in mice. International Immunopharmacology. 14:463-470. https://doi.org/10.1016/j.intimp.2012.09.006
  9. Halliwell B, Aeschbach R, Loliger J and Aruoma OI. (1995). The characterization of antioxidants. Food and Chemical Toxicology. 33:601-617. https://doi.org/10.1016/0278-6915(95)00024-V
  10. He ZQ and Findlay JA. (1991). Constituents of Astragalus membranaceus. Journal of Natural Products. 54:810-815. https://doi.org/10.1021/np50075a009
  11. Im KR, Kim MJ, Jung TK and Yoon KS. (2010). Analysis of isoflavonoid contents in Astragalus membranaceus Bunge cultivated in different areas and at various ages. Korean Society for Biotechnology and Bioengineering Journal. 25:271-276.
  12. Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H and Itakura Y. (1994). Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Medica. 60:417-420. https://doi.org/10.1055/s-2006-959522
  13. Jang YJ, Kim EJ, Kim SY, Lee YH and Park SY. (2016). Changes in physicochemical components of Astragalus membranaceus fermented with Phellinus linteus. The Korean Society of Food Preservation. 23:680-688. https://doi.org/10.11002/kjfp.2016.23.5.680
  14. Jeon YH, Moon JW, Kweon HJ, Jeoung YJ, An CS, Jin HL, Hur SJ and Lim BO. (2010). Effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention of diabetic cataract and retinopathy in streptozotocin-induced diabetes rat model. Korean Journal of Medicinal Crop Science. 18:15-21.
  15. Jeong JW, Lee YC, Jung SW and Lee KM. (1994). Flavor components of citron juice as affected by the extrction method. Korean Journal of Food Science and Technology. 26:709-712.
  16. Jun DH, Cho WA, Lee JB, Jang MJ, You MS, Park JY, Kim SH and Lee JT. (2014). Antioxidant activity of Chestnut(Castanea crenata S.et Z.) bur fermented by Lactobacillus sakei. Journal of Life Science. 24:1193-1199. https://doi.org/10.5352/JLS.2014.24.11.1193
  17. Jun YM, Kim EH, Lim JJ, Kim SH, Kim SH, Lim JD, Cheoi DS, Cheoi YS, Yu CY and Chung IM. (2012). Variation of phenolic compounds contents in cultivated Astragalus membranaceus. Korean Journal of Medicinal Crop Science. 20:447-453. https://doi.org/10.7783/KJMCS.2012.20.6.447
  18. Jung SY. (2018). Cosmetic application of sprouts of Scutellaria baicalensis and Astragalus membranaceus. Ph.D. Thesis. Kyung Hee University. Seoul, Korea. p.1-106.
  19. Kim BH, Jang JO, Lee JH, Park YE, Kim JG, Yoon YC, Jeong SJ, Kwon GS and Lee JB. (2019). Evaluation of the anti-oxidant activity of Pueraria extract fermented by Lactobacillus rhamnosus BHN-LAB 76. Journal of the Korean of Life Science. 29:545-554.
  20. Kim DS, Roh JH, Cho CW and Ma JY. (2012). Analysis of nodakenetin from Samultangs fermented by lactose bacteria strains. Korean Journal of Herbology. 27:35-39. https://doi.org/10.6116/kjh.2012.27.1.35
  21. Kim GH and Bae EK. (1999). Lactic acid bacteria for the preservation of fruit and vegetables. Korean Journal of Food Preservation. 6:245-254.
  22. Kim MJ, Lim KR, Jung TK and Yoon KS. (2007). Anti-aging effect of Astragalus membranaceus root extract. Journal of Society of Cosmetic Scientists of Korea. 33:33-40.
  23. Kim NM, Sung HS and Kim WJ. (1993). Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean of Journal Food science Technology. 25:204-209.
  24. Kim SH, Lee JS, Park KS, Lee JS, Lee HW and Park S. (1999). Liquid culture of basidiomycetes on natural media. Korean Journal of Mycology. 27:373-377.
  25. Kim SK and Lim SD. (2018). Functionality and research trend of probiotics. Food Industry and Nutrition. 23:18-23.
  26. Kim YM, Jeong HJ, Chung HS, Seong JH, Kim HS, Kim DS and Lee YG. (2016). Anti-oxidative activity of the extracts from Houttuynia cordata Thunb. fermented by lactic acid bacteria. Journal of the Korean of Life Science. 26:468-474. https://doi.org/10.5352/JLS.2016.26.4.468
  27. Kitagawa I, Wang H and Yoshikawa M. (1983). Saponin and sapogenol. XXXVII: Chemical constituents of Astragali Radix, the root of Astragalus membranaceus Bunge. (4): Astragalosides VII and VIII. Chemical and Pharmaceutical Bulletin. 31:716-722. https://doi.org/10.1248/cpb.31.716
  28. Lee KJ, Song NY, Roh JH, Liang C and Ma JY. (2013). Analysis of bioconversed-components in fermented Jaeumganghwa-tang by Lactobacillus. Journal of Applied Biological Chemistry. 56: 131-135. https://doi.org/10.3839/jabc.2013.021
  29. Lee SE, Kim YS, Kim JE, Bang JK and Seong NS. (2004). Antioxidant activity of Ulmus davidiana var. Jiponica N and Hemipteleae davidii P. Korean Journal of Medicinal Crop Science. 12:321-327.
  30. Park YE, Kwon GS, Kim BH and Lee JB. (2019). Evaluation of the usefulness of the fermented thistle(Cirsium japonicum) with Lactobacillus rhamnosus BHN-LAB105 for antioxidative and whitening effects. Asian Journal of Beauty and Cosmetology. 17:1-13. https://doi.org/10.20402/ajbc.2018.0250
  31. Pyo YH, Lee TC and Lee YC. (2005). Enrichment of bioactive isoflavones in soymilk fermented with β-glucosidase-producing lactic acid bacteria. Journal Food Research. 38:551-559. https://doi.org/10.1016/j.foodres.2004.11.008
  32. Song HS, Eom SH, Kang YM, Choi JD and Kim YM. (2011). Enhancement of the antioxidant and anti-inflammatory activity of Hizikia fusiforme water extract by lactic acid bacteria fermentation. Korean Journal of Fisheries and Aquatic Sciences. 44:111-117. https://doi.org/10.5657/kfas.2011.44.2.111
  33. Toda S and Shirataki Y. (1999). Inhibitory effects of Astragali radix, crude drug in oriental medicines on lipid peroxidation and protein oxidative modification by copper. Phytotherapy Research. 68:331-333.
  34. Yu Q, Li P, Bi Z, Luo J and Gao X. (2007). Two new saponins from the aerial part of Astragalus membranaceus var. mongholicus. Chinese Chemical Letters. 18:554-556. https://doi.org/10.1016/j.cclet.2007.03.025
  35. Zhong R, Yu M, Liu H, Sun H, Cao Y and Zhoua D. (2012). Effects of dietary Astragalus polysaccharide and Astragalus membranaceus root supplementation on growth performance, rumen fermentation, immune responses, and antioxidant status of lambs. Animal Feed Science and Technology. 174:60-67. https://doi.org/10.1016/j.anifeedsci.2012.02.013