Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement and Conversion of Ginsenoside Contents in Cultured Wild Ginseng Adventitious Root

산삼 부정배양근의 진세노사이드 함량 증진과 성분 변환

  • Kim, Chul Joong (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Choi, Jae Hoo (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Oh, Yeong Seon (Research Institute of Biotechnology, HwajinBioCosmetic) ;
  • Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University) ;
  • Lim, Jung Dae (Department of Bio-Health Convergence, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Lee, Jae Geun (Research Institute of Biotechnology, HwajinBioCosmetic)
  • 김철중 ((주)화진바이오코스메틱) ;
  • 최재후 ((주)화진바이오코스메틱) ;
  • 오영선 ((주)화진바이오코스메틱) ;
  • 성은수 (수원여자대학교 약용식물과) ;
  • 임정대 (강원대학교 바이오헬스융합학과) ;
  • 유창연 (강원대학교 생물자원과학과) ;
  • 이재근 ((주)화진바이오코스메틱)
  • Received : 2020.09.16
  • Accepted : 2020.12.01
  • Published : 2020.12.30
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Abstract

Background: Culturing wild ginseng adventitious root using plant factory technology provides genetic safety and high productivity. This production technology is drawing attention in the fields of functional raw materials and product development. The cultivation method using elicitors is key technology for controlling biomass and increasing secondary metabolites. Methods and Results: Elicitor treatments using methyl jasmonate, pyruvic acid, squalene, β-sistosterol were performed to amplify total ginsenosides (Rb1, Rc, Rb2, Rb3, and Rd) of cultured wild ginseng adventitious root. Thereafter, fermentation and steaming processes were performed to convert total ginsenosides into minor molecular ginsenosides (Rg3, Rk1, and Rg5). The result indicated that methyl jasmonate minimizes the reduction in fresh weight of cultured wild ginseng adventitious root and maximizes total ginsenosides (sum of Rb1, Rc, Rb2, Rb3, and Rd). Ginsenoside conversion results showed a maximum degree of conversion of 131 mg/g. Conclusions: In this study, we demonstrated that the optimal elicitor treatment method increased the content of total ginsenosides, while the steaming and fermentation processing method increased the content of minor ginsenosides.

Keywords

References

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