Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Comparison of hematologic and biochemical values in htPA transgenic pigs

사람 조직 플라스미노겐 활성인자 생산용 형질전환 돼지에서의 혈액학적 성상 비교

  • Park, Mi-Ryung (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hwang, In-Sul (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Seunghoon (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Hwi-Cheul (Animal Biotechnolgy Division, National Institute of Animal Science, Rural Development Administration)
  • 박미령 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 황인설 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이승훈 (농촌진흥청 국립축산과학원 동물바이오공학과) ;
  • 이휘철 (농촌진흥청 국립축산과학원 동물바이오공학과)
  • Received : 2020.10.05
  • Accepted : 2020.12.04
  • Published : 2020.12.31
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Abstract

Pigs have been used widely in biomedical research owing to their physiologic and anatomic similarities to humans. Analysis of the hematologic and biochemical values in pigs is an important basis for biomedical research and veterinary clinical diagnosis, but research on transgenic pigs has been sparse. This study was conducted to obtain basic data on transgenic pigs and to describe and compare the reference values for hematologic and biochemical parameters in human tissue plasminogen activator (htPA) transgenic pigs vs normal pigs. Blood samples were obtained from 7 normal LY (Landrace-Yorkshire crossbred) pigs and 8 transgenic pigs and 16 hematologic and 15 serum biochemical parameters were tested. Among the hematologic parameters tested, significant differences were observed in the red blood cells (RBC), mean red blood cell hemoglobin (MCH), and lymphocytes (LYM), between the non-transgenic and transgenic pigs. Among the biochemical parameters tested, the blood urea nitrogen (BUN), total protein (TP), cholesterol (CHOL), alanine aminotransferase (ALT), creatinine (CREA), gamma glutamyl transpeptidase (GGT), globin (GOB), and amylase (AMYL) showed significant differences between the two groups. Thus, the values determined in this study can be used as basic reference values for transgenic pigs and will contribute to their use in biomedical research.

해부학적, 생리학적으로 인간과 유사한 특성을 지닌 돼지를 이용한 실험은 의학적 분야에서 폭넓게 이용되고 있다. 돼지에서 혈액의 일반적인 성상과 이화학적 수치는 의학적 연구 및 수의학적 치료에서도 중요한 부분으로 인정되고 있으나, 형질전환 돼지에 대한 연구는 미비한 실정이다. 따라서, 본 연구에서는 htPA 형질전환 돼지의 혈액을 이용한 일반성상 및 이화학적 성상을 비교 분석하여 형질전환 돼지에 대한 기초 자료로 활용하고자 조사하였다. 일반돼지 7(LY)두와 형질전환 돼지 8(LY)두의 혈액을 각각 분석하였다. 혈액의 일반 성상은 16종을 분석하였으며, 혈청을 이용한 이화학 분석의 경우 15종 항목을 조사하였다. 그 결과 혈액의 일반 성상 분석에서는 적혈구(RBC), 평균적혈구 혈색소량(MCH)과 임파구(LYM)에서 두 그룹간 유의적 차이를 나타내었다. 이화학적 성상 분석에서는 혈중뇨소질소(BUN), 총단백질(TP), 콜레스테롤(CHOL), (ALT), 크레아틴(CREA), 감마글루타밀전이효소(GGT), 글로빈(GOB) 그리고 아밀라아제(AMYL)가 두 그룹간 유의적 차이를 나타내었다. 앞으로 지속적인 형질전환 돼지에 대한 생체정보를 조사함으로써, 기초 자료로 이용할 수 있을 뿐만 아니라, 더 나아가 의학적 연구 분야에 적용 시 참고할 수 있을 것으로 여겨진다.

Keywords

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