Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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In vivo assessment of the nutritional value of Allomyrina dichotoma larva protein as future protein resource

미래 식량자원으로써 장수풍뎅이 유충의 영양학적 평가

  • Choi, Young-Jin (Department of Food Science, College of Biomedical and Health Sciences, Konkuk University) ;
  • Fan, Meiqi (Department of Food Science, College of Biomedical and Health Sciences, Konkuk University) ;
  • Kim, Eun-Kyung (Department of Food Science and Nutrition, College of Health Science, Dong-A University) ;
  • Lee, Seul-bi (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Hwang, Yeon-hyeon (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Jang, Yeong-Ho (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Bae, Sung-Mun (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
  • 최영진 (건국대학교 바이오융합과학부 식품학전공) ;
  • 범미기 (건국대학교 바이오융합과학부 식품학전공) ;
  • 김은경 (동아대학교 건강과학대학 식품영양학과) ;
  • 이슬비 (경상남도농업기술원 환경농업연구과) ;
  • 황연현 (경상남도농업기술원 환경농업연구과) ;
  • 장영호 (경상남도농업기술원 환경농업연구과) ;
  • 배성문 (경상남도농업기술원 환경농업연구과)
  • Received : 2020.09.17
  • Accepted : 2020.11.17
  • Published : 2020.12.31
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Abstract

Edible insects might be used as a means to solve food insecurity caused by population growth. Many studies have investigated the biological activity of insects; however, few studies have investigated the nutritional value of insects. Therefore, the aim of this study was to investigate the nutritional value of Allomyrina dichotoma larva protein (ADP) as a source of protein replacement. In vivo studies were conducted to determine the food efficiency ratio (FER), protein efficiency ratio (PER), and true digestibility (TD) of ADP. Experiments were conducted in 3 groups of 8 animals per group using twenty-four 4-week-old SD rats. The experimental groups included the general diet group (Con), in which 20% of the total Kcal in the diet was composed of casein protein, and the ADP group (ADP), in which 20% of the total Kcal was composed of ADP protein, and a non-protein diet group (NP) to measure the protein (metabolic fecal nitrogen) excreted by metabolic processes in the body. As a result of this experiment, we found that the FERs were 0.52 and 0.41 in the casein protein intake (Con) and ADP groups, respectively, thus showing a significantly lower level in the ADP group. The PERs of ADP and Con were 2.39 and 2.63, respectively. The TD of Con and ADP were 91% and 80%, respectively.

식용 곤충은 인구증가에 따른 식량 문제를 해결하기 위한 하나의 방법으로 제시되며 최근 곤충의 생리활성 기능과 관련한 연구가 많이 수행되고 있다. 반면 식품으로서 활용 가능한 기초연구는 부족한 실정으로 본 연구에서 장수풍뎅이 유충의 단백질 대체 자원으로서 영양학적 기능을 규명하기 위한 실험을 실시하여 장수풍뎅이 유충 단백질(ADP)의 식이 효율, 단백질 효율, 순 소화율 등을 동물실험을 통해 알아보았다. 4주령 SD-rat 24마리를 이용하여 각 그룹 당 8마리 3그룹으로 실험을 실시하였다. 실험군은 사료 내 전체 kcal 20%가 Casein 단백질로 구성된 사료를 식이 하는 일반 식이군(Con), 전체 kcal 20%가 장수풍뎅이 유충의 단백질로 구성된 사료를 식이 하는 장수풍뎅이 유충 단백질식이군(ADP), 그리고 체내 대사작용에 의해 배설되는 단백질(metabolic fecal nitrogen)을 측정하기 위하여 단백질 무 첨가 식이군(NP, non-protein diet)으로 설정했다. 4주간 식이를 공급하여 장수풍뎅이 유충 단백질의 식이 효율(Food Efficiency Ratio, FER), 단백질 효율(Protein Efficiency Ratio, PER), 순 소화율(true digestibility, TD)을 평가했다. 본 실험 결과 식이 효율은 Con군에서 0.52, 장수풍뎅이 유충 단백질 섭취군에서 0.41로 ADP에서 유의적으로 낮은 수준을 보였다. 단백질 효율은 ADP군에서 2.39, Con군에서 2.63으로 ADP군은 Con군에 비해 낮았으나 유의적인 차이는 없었다. 순 소화율은 Con군에서 91%, ADP군에서 80%로 나타났으며 통계적인 유의적 차이는 없었다. 장수풍뎅이 유충의 순 소화율은 80%로 나타났으며 콩과류의 단백질 순 소화율 64-77%에 비해 높은 수준을 보였다.

Keywords

References

  1. Bhatty N, Gilani AH, Nagra SA. Effect of cooking and supplementation on nutritional value of gram (Cicer arietinum). Nutr. Res. 20: 297-307 (2000) https://doi.org/10.1016/S0271-5317(99)00161-X
  2. Bukkens SGF. The nutritional value of edible insects. Ecol. Food Nutr. 36: 287-319 (1997) https://doi.org/10.1080/03670244.1997.9991521
  3. Choi YH, Lee KY, Yang KM, Jeong YM, Seo JS. Effect of larva extract of Allomyrina dichotoma on carbon tetrachloride-induced hepatotoxicity in mice. J. Korean Soc. Food Sci. Nutr. 35: 1349-1355 (2006) https://doi.org/10.3746/JKFN.2006.35.10.1349
  4. Food and Agricultural Organization of the United Nations. Insects for Food and Feed. Available from: http://www.fao.org/edible-insects/en. Accessed Sep. 19, 2016
  5. Ghosh S, Lee SM, Jung C, Meyer-Rochow VB. Nutritional composition of five commercial edible insects in South Korea. J. Asia Pac. 20: 686-694 (2017)
  6. Gertjan S. The protein digestibility-corrected amino acid score. J. Nutr. 130: 1865S-1867S (2000) https://doi.org/10.1093/jn/130.7.1865S
  7. Kim J, Yun EY, Park SW, Goo TW, Seo M. Allomyrina dichotoma larvae regulate food intake and body weight in high fat dietinduced obese mice through mTOR and Mapk signaling pathways. Nutrients, 8: 100-111 (2016). https://doi.org/10.3390/nu8020100
  8. Kim M, Youn K, Yun EY, Hwang JS, Ahn MR, Jeong WS, Jun M. Effects of solvent fractions of Allomyrina dichotoma larvae through the inhibition of in vitro BACE1 and β-amyloid (25-35)-induced toxicity in rat pheochromocytoma PC 12 cells. Entomol. Res. 44: 23-30 (2014) https://doi.org/10.1111/1748-5967.12046
  9. Liu J, Klebach M, Visser M, Hofman, Z. Amino acid availability of a dairy and vegetable protein blend compared to single casein, whey, soy, and pea proteins: A double-blind, cross-over trial. Nutrients. 11: 2613 (2019) https://doi.org/10.3390/nu11112613
  10. Seena S, Sridhar KR. Nutrient composition and biological evaluation of an unconventional legume, Canavalia cathartica of mangroves. Int. J. Food Sci. 55: 615-625 (2004) https://doi.org/10.1080/09637480500085622
  11. Shin CS, Kim DY, Shin WS. Characterization of chitosan extracted from Mealworm Beetle (Tenebrio molitor, Zophobas morio) and Rhinoceros Beetle (Allomyrina dichotoma) and their antibacterial activities. Int. J. Biol. Macromol. 125: 72-77 (2019) https://doi.org/10.1016/j.ijbiomac.2018.11.242
  12. Silva LE, Barbosa MC, Hiane PA, Braga Neto JA, Macedo ML. R. Proteins of Bacuri almonds: nutritional value and in vivo digestibility. J. Food Sci. Technol. 34: 55-61 (2014) https://doi.org/10.3969/j.issn.2095-6002.2016.01.009
  13. Suh HJ, Kim SR, Lee KS, Park S, Kang SC. Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda: Insecta) larvae. J. Photochem. Photobiol. 99: 67-73 (2010) https://doi.org/10.1016/1010-6030(96)04376-6
  14. Yoon YI, Chung MY, Hwang JS, Han MS, Goo TW, Yun EY. Allomyrina dichotoma (Arthropoda: Insecta) larvae confer resistance to obesity in mice fed a high-fat diet. Nutrients. 7: 1978-1991 (2015) https://doi.org/10.3390/nu7031978