Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Evaluation of the Sterilization Effect of a Plasma Generator with a Flexible Electrode Structure on Staphylococcus aureus and Pseudomonas aeruginosa

황색포도알균과 녹농균에 대한 유연전극 구조를 갖는 플라즈마 발생기의 멸균효과 평가

  • Park, Chul (Department of Biomedical Laboratory Science, Gwangju Health University) ;
  • Lee, Hyeok Jae (Department of Biomedical Laboratory Science, Gwangju Health University)
  • 박철 (광주보건대학교 임상병리과) ;
  • 이혁재 (광주보건대학교 임상병리과)
  • Received : 2020.10.28
  • Accepted : 2020.11.25
  • Published : 2020.12.31
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Abstract

In this study, the sterilization ability of S. aureus and P. aeruginosa was evaluated using a plasma generator with a flexible electrode structure. Both strains were prepared at a concentration of 1.5×106 CFU/mL and inoculated and spread evenly on two medium plates. The medium were kept at a distance of 3 cm and 9 cm from the plasma generator and were plasma discharged from 30 sec to 10 minutes. The growth of colonies on the media, were subsequently compared with the control group. The mean colonies of S. aureus formed at a 3 cm distance were 9.2×102 (log value 2.96) CFU/mL for the 5 min discharge period and 8.0×10 (1.90) CFU/mL for the 10 min discharge period. When the medium was exposed for 5 min and 10 min at a 9 cm distance, the mean colonies of S. aureus formed were 2.16×103 (3.33) and 2.4×102 (2.38) CFU/mL, respectively. The medium containing P. aeruginosa kept at a 3 cm distance and exposed to 3, 5, 10-minute discharge, did not form any colonies. When kept at a 9 cm distance for 3 minutes, 6.0×102 (2.78) CFU/mL mean colonies were formed but no colonies were formed at exposure periods of 5 and 10 minutes. This enhanced sterilization effect was confirmed in experiments of S. aureus and P. aeruginosa using TiO2.

본 연구에서 유연전극 구조의 플라즈마 발생기를 이용하여 S. aureus와 P. aeruginosa의 살균 능력을 평가하였다. 두 균주 모두 1.5×106 CFU/mL 농도의 부유액으로 조제하여 배지에도말 후, 플라즈마 발생기로부터 3 cm, 9 cm 떨어뜨려 15초 간격으로, 30초부터 120초까지 그리고 3분, 5분, 10분 동안 플라즈마를 방전하여 배지에 형성된 colony를 대조군과 비교 하였다. 3 cm 떨어뜨린 배지에서 형성된 S. aureus의 평균 집락은 5분 방전했을 때 9.2×102 (log 값 2.96) CFU/mL 이었고, 10분 방전했을 때는 8.0×10 (1.90) CFU/mL이 형성되었다. 9 cm 떨어뜨린 배지에 5분 또는 10분 동안 방전했을 때 형성된 S. aureus의 평균 집락은 각각 2.16×103 (3.33)과 2.4×102 (2.38) CFU/mL이 형성되었다. 3 cm 떨어뜨려 3분, 5분, 10분간 방전하였을 때 P. aeruginosa는 완전히 사멸되어 colony가 형성되지 않았다. 9 cm 떨어뜨려 3분 방전했을 때 P. aeruginosa는 6.0×102 (2.78) CFU/mL이 형성되었으나, 5분, 10분간 방전하였을 때는 완전히 사멸되어 colony가 형성되지 않았다. 또한 TiO2를 이용한 S. aureus와 P. aeruginosa 실험에서 더 나은 살균 효과를 확인할 수 있었다.

Keywords

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