Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Evaluation of the acute toxicity of theoredoxin (TRX) transgenic soybean to Daphnia magna

  • Oh, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Min, Seok-Ki (Korea Testing & Research Institute) ;
  • Kim, Jae Kwang (Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University) ;
  • Park, Jung-Ho (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Gi (Bio-evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Soo Yun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2020.09.01
  • Accepted : 2020.09.18
  • Published : 2020.12.01
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Abstract

Theoredoxin (TRX) transgenic soybeans were developed using the human Theoredoxin gene under the control of the ��-conglycinin promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. This study was done to assess the acute toxicity of a genetically modified (GM) soybean using the fresh water planktonic crustacean Daphnia magna. The acute toxicity effect of the TRX soybean and non-GM soybean (Gwangan) on D. magna was investigated at different concentrations (0, 156, 313, 625, 1,250, 2,500, and 5,000 mg·L-1). The TRX soybean used for the test was confirmed to express the TRX/PAT genes by PCR and enzyme-linked immunosorbent assay (ELISA). D. magna feeding tests showed no significant differences in the cumulative immobility or an abnormal response with either the TRX soybean or non-GM soybean. The feeding study showed a similar abnormal response and cumulative immobility of the D. magna between the TRX soybean and Gwangan treatments. Additionally, the 48 h-EC50 values for the TRX and Gwangan soybeans were 755.6 and 778 mg·L-1, respectively. The soybean NOEC (no observed effect concentration) value for D. magna was suggested to be 156 mg·L-1. These results suggest that there is no significant difference in toxicity to Daphnia magna between the TRX soybean and its non-GM counterpart.

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References

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