Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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In vitro fertilization using sex-sorted boar sperm mediated by magnetic nanoparticles

  • Chung, Hakjae (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Baek, Sunyoung (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Sa, Soojin (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Youngshin (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hong, Joonki (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Eunseok (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Jihwan (Dairy Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Ha, Seungmin (Dairy Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Son, Jungho (Chungnam Techno Park) ;
  • Lee, Seunghwan (Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam University) ;
  • Choi, Inchul (Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam University) ;
  • Kim, Kyungwoon (Planning and Coordination Division, National Institute of Animal Science, Rural Development Administration)
  • Received : 2020.09.25
  • Accepted : 2020.11.12
  • Published : 2020.12.01
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Abstract

A wide range of techniques have been developed to separate X or Y- chromosome-bearing sperm. In particular, bovine semen sex-sorted by using flow cytometry based on differences in the amount of DNA between X and Y chromosome bearing sperm is used in dairy farms. The first piglets were produced using sex-sorted sperm 30 years ago. However, sexed sperm have not been commercially available in pigs because the flow cytometry technique is not capable of sorting the high number of sperm required for porcine artificial insemination (AI), and the prolonged exposure to an electrical filed might damage to the DNA in sperm. The purpose of this study was to evaluate a boar sperm sorting method based on magnetic nanoparticles. A flow cytometer assay verified the efficacy of the magnetic nanoparticles (> 90% of sex-sorted sperm). In addition, a duplex polymerase chain reaction (PCR) assay using sex chromosome specific genes including SRY (sex-determining region Y; male), ZFY (zinc finger protein Y-linked; male), and ZFX (zinc finger protein X-linked; female) showed that in vitro fertilized porcine embryos by X and Y-chromosome bearing sperm were 100% female (40/40) and 72% female (35/48), respectively, at 8-cell or morula stages, suggesting that the sex-sorted sperm were fertile. In conclusion, our findings suggest that the sex-sorted method based on magnetic nanoparticles can be utilized for porcine sex-sorted AI.

Keywords

Acknowledgement

연구는 농촌진흥청 연구사업(세부가제번호: PJ01359301)의 지원에 의해 수행되었습니다.

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