The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Changes in Bioluminescence of Omphalotus japonicus Mycelia under Environmental Stress Conditions

환경 스트레스 조건에 따른 화경버섯 균사의 생물발광 변화

  • Park, Mi-Jeong (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science) ;
  • Lee, Hyorim (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science) ;
  • Ryoo, Rhim (Division of Special Forest Products, Department of Forest Bioresources, National Institute of Forest Science)
  • 박미정 (국립산림과학원 산림생명자원연구부 산림소득자원연구과) ;
  • 이효림 (국립산림과학원 산림생명자원연구부 산림소득자원연구과) ;
  • 유림 (국립산림과학원 산림생명자원연구부 산림소득자원연구과)
  • Received : 2020.12.14
  • Accepted : 2020.12.21
  • Published : 2020.12.31
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Abstract

Bioluminescence refers to the production and emission of light in living organisms. This phenomenon arises from luciferase-catalyzed oxidation reaction of luciferin. Bioluminescence is widely observed in marine vertebrates and invertebrates, as well as in some microorganisms and fungi. To date, approximately 80 species of fungi have been reported to be luminous. One such example is Omphalotus japonicus, which is a luminous fungus found in Korea. In this study, we examined the bioluminescence of Omphalotus japonicus mycelia. Light emission was detected at the edges of mycelia grown on solid agar medium. Notably, the intensity of bioluminescence was found to be significantly enhanced following wound induction. The increase in light intensity peaked at 3 h after mechanical damage. We also investigated the effects of extreme temperatures on bioluminescence. Unlike mechanical damage, high and low temperatures repressed the light emission from mycelia. Further investigations are required to reveal the physiological and ecological properties of fungal bioluminescent responses to environmental stresses.

생물발광은 생물체 내에서 빛이 방출되는 현상을 말한다. 이 현상은 루시페라아제(luciferase)에 촉매되는 루시페린(luciferin)의 산화 반응에 의해 나타난다. 생물발광은 균류를 포함하여 다양한 생물 종에서 나타남이 알려져 있다. 현재까지 약 80여 종의 균류에서 생물발광이 관찰됨이 보고 되었다. 화경버섯(Omphalotus japonicus)은 한국에 자생하는 대표적인 생물발광버섯이다. 본 연구에서는 화경버섯 균사의 생물발광에 대하여 조사하였다. 그 결과, 고체 배지에서 자란 화경버섯 균사체의 가장자리 부근에서 중심부에 비해 높은 발광이 관찰되었다. 또한 균사 표면에 상처를 내자 해당 부위에서 강한 생물발광이 관찰되었다. 이러한 생물발광의 증가는 처리 후 3시간에 최대로 나타났다. 다음으로 극단적인 온도 스트레스에 의한 생물 발광의 변화에 대해서도 살펴보았다. 그 결과, 외부 자극에 의한 상처 스트레스와 달리 고온이나 저온 스트레스에 의해 균사의 생물 발광이 억제됨을 확인하였다. 환경 스트레스에 의해 일어나는 생물발광 변화의 생리학적 및 생태학적 의미를 밝히기 위해 더 많은 연구가 필요할 것이다.

Keywords

Acknowledgement

This work was supported by the biological toxicity research grants (KNA 1-3-2, 19-5 and KNA 1-3-3, 20-3) from the National Institute of Forest Science provided by the Korea Forest.

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