The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characterization of Green Mold Contamination caused by Penicillium brevicompactum in Hypsizygus marmoreus

느티만가닥버섯에서 Penicillium brevicompactum에 의해 유발된 곰팡이 오염 특성

  • Kim, Min-Keun (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Sim, Soon-Ae (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Kim, Ah-Young (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Kwon, Jin-Hyeuk (Environmental Agriculture Research Division, Gyeongsangnam-do Agricultural Research and Extension Service) ;
  • Chang, Young-Ho (Crop Research Division, Gyeongsangnam-do Agricultural Research and Extension Service)
  • 김민근 (경상남도농업기술원 환경농업연구과) ;
  • 심순애 (경상남도농업기술원 환경농업연구과) ;
  • 김아영 (경상남도농업기술원 환경농업연구과) ;
  • 권진혁 (경상남도농업기술원 환경농업연구과) ;
  • 장영호 (경상남도농업기술원 작물연구과)
  • Received : 2020.09.02
  • Accepted : 2020.11.30
  • Published : 2020.12.31
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Abstract

Beech mushroom (Hypsizygus marmoreus) is one of the most popular mushrooms in Japan, China, and Korea because of its delicious taste, nutritious value, and unique crunchy texture. In 2018-2019, unusual signs on the mushroom were observed in mushroom farms in Gyeongnam Province. The main signs were the inhibition of mycelial growth and primordial formation on the surface of mushroom media. When green mold caused contamination at early stage, the rate of pinhead formation by the mushroom greatly decreased to 46.5-71.5%. Conidia of the causal green mold were ellipsoidal, dark green, and measured 2.0-3.1㎛ long. The optimum temperature for mycelial growth of the green mold was 25℃. The phylogenetic tree obtained from the internal transcribed spacer (ITS) rDNA sequences showed that the isolated green mold corresponded to Penicillium brevicompactum (100.0%). This is the first report of green mold contamination caused by P. brevicompactum on H. marmoreus.

느티만가닥버섯은 일본에서 가장 많이 생산하고 소비되는 버섯으로 영양학적 가치, 우수한 식감 및 저장성 때문에 국내에서도 점진적으로 생산량이 늘어나고 있다. 2018년과 2019년 사이 경남지역 느티만가닥버섯 재배농가에서 자실체 발생이 불균일하며 수량이 감소하는 피해가 확인되었다. 분리한 오염균은 균총의 전체 표면에 회색을 띠는 진녹색포자를 형성하였다. 분리 균주의 균사생장 적합온도는 25℃로 확인되었으며 비교적 느리게 자라는 특성을 보여주었다. 분리 균주는 ITS rDNA 염기서열 분석결과 Penicillium brevicompactum과 100.0%의 상동성을 나타내었고, P. brevicompactum F51로 동정 및 명명하였다. 분리 균주를 처리한 배지는 농가에서 수집된 시료와 동일하게 버섯균사의 재생을 저해하면서 발이가 46.5-71.5% 수준까지 감소되었으며, 수량은 최대 75.6%까지 감소하였다. 이러한 결과는 P. brevicompactum이 느티만가닥버섯 재배과정에서 피해를 일으킨다는 사실을 국내에서 처음 보고하는 것이다.

Keywords

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