The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Seven Yeast Strains Isolated from Freshwaters for the First Record in Korea

  • Jeon, Yu Jeong (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Park, Sangkyu (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Hwang, Hyejin (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Park, Yeong Hwan (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Cheon, Wonsu (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Goh, Jaeduk (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Chung, Namil (Fungi Research Team, Nakdonggang National Institute of Biological Resources) ;
  • Mun, Hye Yeon (Fungi Research Team, Nakdonggang National Institute of Biological Resources)
  • Received : 2020.08.25
  • Accepted : 2020.09.21
  • Published : 2020.12.31
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Abstract

This study focused on the isolation and characterization of wild yeasts in Korea. The yeasts were identified by phylogenetically analyzing the D1/D2 domains of the 26S rDNA regions. Consequently, we identified seven strains, NNIBRFG856, NNIBRFG3732, NNIBRFG3734, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049, which were confirmed to be Kabatiella microsticta, Pichia membranifaciens, Candida vartiovaarae, Candida sake, Debaryomyces hansenii, Candida railenensis, and Schwanniomyces polymorphus, respectively, all of them being new in Korea. Morphological and cultural characteristics of these yeast species were investigated. None of the strains formed ascospores or pseudomycelia. Moreover, these yeasts grew in a pH range of 4-8. NNIBRFG3732, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049 were halotolerant or halophilic, and NNIBRFG3732, NNIBRFG3734, and NNIBRFG6049 grew in vitamin-free medium. NNIBRFG3732, NIBRFG3739, and NNIBRFG6049 grew at 35 ℃, but not at 40 ℃.

Keywords

Acknowledgement

This work was supported by a grant from the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202001105).

References

  1. Han SM, Lee SY, Lee HB, Lee JS. Isolation of wild yeasts from soils of reed fields in Seocheon-gun County, Chungcheongnam-do, South Korea, and characterization of unrecorded yeasts. Kor J Mycol 2017;45:234-40. https://doi.org/10.4489/KJM.20170028
  2. Nagahama T. Yeast biodiversity in freshwater, marine water and deep-sea environments. In: Rosa CA, Gabor P, editors. Biodiversity and ecophysiology of yeasts. Berlin: Springer; 2006. p. 241-61.
  3. Wang Y, Zhao Y-C, Fan L-L, Xia X-D, Li Y-H, Zhou J-Z. Identification and characterization of Picha membranifaciens Hmp-1 isolated from spoilage blackberry wine. J Integr Agric 2018;17:2126-36. https://doi.org/10.1016/s2095-3119(18)62027-1
  4. Veiga A, Madeira-Lopes VA. Effects of weak acid preservatives on the growth and thermal death of the yeast Pichia membranifaciens in a commercial apple juice. IInt J Food Microbiol 2000;56:145-51. https://doi.org/10.1016/S0168-1605(99)00191-9
  5. Abdel-Raheam HEF, Farag MKA, Ragab WSM, Abdel-Rahman EA, Ghonaimy AG. Isolation and identification of amylolytic yeasts from agricultural and industrial wastes. Assiut J of Agric Sci 2011;42:55-64.
  6. Breuer U, Harms H. Debaryomyces hansenii - an extremophilic yeast with biotechnological potential. Yeast 2006;23:415-37. https://doi.org/10.1002/yea.1374
  7. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  8. Hyun SH, Han SM, Lee JS. Characteristics and physiological functionalities of unrecorded yeasts from wild flowers of Seonyudo in Jeollabuk-do, Korea. Korean J Microbiol Biotechnol 2014;42:402-6. https://doi.org/10.4014/kjmb.1409.09001