Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Antiobesity and Antidiabetic Effects of Polyherbal Extract with Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in High Fat Diet-induced Obesity Mice

고지방식이 유도 비만 마우스에서 창출, 지모, 육계, 목단피 혼합추출물의 항비만 및 항당뇨 효능 연구

  • Jung, Su Min (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Seol, Young Hyun (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Chun, Ka Yoon (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Park, Min Ha (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Liu, Yi (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Kang, Seok Yong (Korean Medicine R&D Institute, Dongguk University) ;
  • Park, Yong-Ki (Department of Herbology, College of Korean Medicine, Dongguk University) ;
  • Jung, Hyo Won (Department of Herbology, College of Korean Medicine, Dongguk University)
  • 정수민 (동국대학교 한의과대학 본초학교실) ;
  • 설영현 (동국대학교 한의과대학 본초학교실) ;
  • 전가윤 (동국대학교 한의과대학 본초학교실) ;
  • 박민하 (동국대학교 한의과대학 본초학교실) ;
  • 유이 (동국대학교 한의과대학 본초학교실) ;
  • 강석용 (동국대학교 한방신약개발센터) ;
  • 박용기 (동국대학교 한의과대학 본초학교실) ;
  • 정효원 (동국대학교 한의과대학 본초학교실)
  • Received : 2020.10.28
  • Accepted : 2020.12.04
  • Published : 2020.12.30
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Abstract

Objectives: In this study, we investigated the antiobesity and antidiabetic effects of polyherbal extract, DM2 consisting of Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in high fat diet-induced obesity mice. Methods: DM2 extract was prepared with a hot water. Six-week-old male C57BL/6N mice were fed a high-fat diet (HFD) for 8 weeks and then administrated with DM2 extract (500 mg/kg, p.o.) for 4 weeks. The changes of physiological markers, body weight (BW), food and water intakes, and the levels of fasting blood glucose (FBG) were measured once a week for 4 weeks in mice. The the serum levels of glucose, insulin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (T-CHO), triglyceride, and low density lipoprotein cholesterol in sera were measured in mice using autometic chemical analyzer and enzyme linked immunosorbant assay. We also observed the histological changes of liver and pancreatic tissues with Hematoxylin & Eosin staining. Results: In physiological change, the increases of BW, calorie intake, and FBG in HFD-induced obese mice were significantly decreased after administration of DM2 extract for 4 weeks. The decrease of water intake was significantly increased in DM2 extract-administrated mice. In serological change, the administration of DM2 extract in obesity mice was significantly decreased the serum levels of glucose, insulin, T-CHO, AST, and ALT levels. We also found that DM2 extract inhibited the increase of lipid droplets in liver and the structural destruction of pancreatic tissues in obesity mice. Conclusion: Our study demonstrated that DM2 extract has antiobesity antidiabetic effects with body weight loss, decrease of glucose and insulin levels, and lipid accumulation on liver tissue.

Keywords

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