대한의생명과학회지 (Biomedical Science Letters)

  • 제26권4호
  • /
  • Pages.267-274
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  • 2020
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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The Anti-inflammatory Mechanism of Protaetia brevitarsis Lewis via Suppression the Activation of NF-κB and Caspase-1 in LPS-stimulated RAW264.7 Cells

  • Myung, Noh-Yil (Department of Oriental Medicine and Healthcare, Wonkwang Digital University) ;
  • Ahn, Eun-Mi (Department of Biotechnology and Convergence, Daegu Haany University) ;
  • Kim, Su-Jin (Department of Biotechnology and Convergence, Daegu Haany University)
  • 투고 : 2020.11.15
  • 심사 : 2020.12.08
  • 발행 : 2020.12.31
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초록

The larva of Protaetia brevitarsis Lewis (P. brevitarsis), edible insect, is traditionally consumed as alternative source of nutrients and has various health benefits. However, the exact pharmaceutical effects of P. brevitarsis on inflammatory response are still not well understood. Thus, we investigated the anti-inflammatory effects and mechanisms of P. brevitarsis in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. We investigated the effects of P. brevitarsis on the expression levels of inflammatory-related genes, including inflammatory cytokines, prostaglandin E2 (PGE2), cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) in LPS-stimulated RAW264.7 cells. To understand the anti-inflammatory mechanism of P. brevitarsis, we explored the regulatory effect of P. brevitarsis on nuclear factor (NF)-κB and caspase-1 activation. The findings of this study demonstrated that P. brevitarsis inhibits the LPS-induced inflammatory cytokine and PGE2 levels, as well as COX-2 and iNOS expression. Moreover, we confirmed that the anti-inflammatory effect of P. brevitarsis occurs via suppression of the activation of NF-κB and caspase-1. Conclusively, these findings provide experimental evidence that P. brevitarsis may be useful candidate for the treatment of inflammatory-related diseases.

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참고문헌

  1. Ahn EM, Myung NY, Jung HA, Kim SJ. The ameliorative effect of Protaetia brevitarsis Larvae in HFD-induced obese mice. Food Science and Biotechnology. 2019. 28: 1177-1186. https://doi.org/10.1007/s10068-018-00553-w
  2. Beutler B. Tlr4: central component of the sole mammalian LPS sensor. Current Opinion in Immunology. 2000. 12: 20-26. https://doi.org/10.1016/S0952-7915(99)00046-1
  3. Birrell MA, Hardaker E, Wong S, McCluskie K, Catley M, De AJ, Newton R, Haj-Yahia S, Pun KT, Watts CJ, Shaw RJ, Savage TJ, Belvisi MG. Ikappa-B kinase-2 inhibitor blocks inflammation in human airway smooth muscle and a rat model of asthma. American Journal of Respiratory and Critical Care Medicine. 2005. 172: 962-971. https://doi.org/10.1164/rccm.200412-1647OC
  4. Edwards MR, Strong K, Cameron A, Walton RP, Jackson DJ, Johnston SL. Viral infections in allergy and immunology: How allergic inflammation influences viral infections and illness. The Journal of Allergy and Clinical Immunology. 2017. 140: 909-920. https://doi.org/10.1016/j.jaci.2017.07.025
  5. Fedenko ES, Elisyutina OG, Filimonova TM, Boldyreva MN, Burmenskaya OV, Rebrova OY, Yarilin AA, Khaitov RM. Cytokine gene expression in the skin and peripheral blood of atopic dermatitis patients and healthy individuals. Self Nonself. 2011. 2: 120-124. https://doi.org/10.4161/self.2.2.16939
  6. Guo H, Callaway JB, Ting JP. Inflammasomes: mechanism of action, role in disease, and therapeutics. Nature Medicine. 2015. 21: 677-687. https://doi.org/10.1038/nm.3893
  7. Han NR, Moon PD, Kim NR, Kim HY, Jeong HJ, Kim HM. Schisandra chinensis and Its Main Constituent Schizandrin Attenuate Allergic Reactions by Down-Regulating Caspase-1 in Ovalbumin-Sensitized Mice. The American Journal of Chinese Medicine. 2017. 45: 159-172. https://doi.org/10.1142/S0192415X17500112
  8. Kim SJ, Kim MC, Um JY, Hong SH. The beneficial effect of vanillic acid on ulcerative colitis. Molecules. 2010. 15: 7208-7217. https://doi.org/10.3390/molecules15107208
  9. Kuida K, Lippke JA, Ku G, Harding MW, Livingston DJ, Su MS, Flavell RA. Altered cytokine export and apoptosis in mice deficient in interleukin-1B converting enzyme. Science. 1995. 267: 2000-2002. https://doi.org/10.1126/science.7535475
  10. Lee YS, Han SB, Ham HJ, Park JH, Lee JS, Hwang DY, Jung YS, Yoon DY, Hong JT. IL-32γ suppressed atopic dermatitis through inhibition of miR-205 expression via inactivation of nuclear factor-kappa B. Journal of Allergy and Clinical Immunology. 2020. 146: 156-168. https://doi.org/10.1016/j.jaci.2019.12.905
  11. Liu YC, Zou XB, Chai YF, Yao YM. Macrophage polarization in inflammatory diseases. International Journal of Biological Sciences. 2014. 10: 520-529. https://doi.org/10.7150/ijbs.8879
  12. Liu Y, Duan C, Chen H, Wang C, Liu X, Qiu M, Tang H, Zhang F, Zhou X, Yang J. Inhibition of COX-2/mPGES-1 and 5-LOX in macrophages by leonurine ameliorates monosodium urate crystal-induced inflammation. Toxicology and Applied Pharmacology. 2018. 15; 351: 1-11.
  13. Rumzhum NN, Ammit AJ. Cyclooxygenase 2: its regulation, role and impact in airway inflammation. Clinical & Experimental Allergy. 2016. 46: 397-410. https://doi.org/10.1111/cea.12697
  14. Shin WB, Dong X, Kim YS, Park JS, Kim SJ, Go EA, Kim EK, Park PJ. Anti-inflammatory Effects of Batillaria multiformis Water Extracts via NF-κB and MAPK Signaling Pathways in LPS-Induced RAW 264.7 Cells. Advances in Experimental Medicine and Biology. 2019. 1155: 1001-1014. https://doi.org/10.1007/978-981-13-8023-5_83
  15. Siegmund B, Lehr HA, Fantuzzi G, Dinarello CA. IL-1 beta converting enzyme (caspase-1) in intestinal inflammation. Proceedings of the National Academy of Sciences of the United States of America. 2001. 98: 13249-13254. https://doi.org/10.1073/pnas.231473998
  16. Sultuybek GK, Soydas T, Yenmis G. NF-κB as the mediator of metformin's effect on ageing and ageing-related diseases. Clinical and Experimental Pharmacology and Physiology. 2019. 46: 413-422. https://doi.org/10.1111/1440-1681.13073
  17. Trefzer U, Hofmann M, Sterry W, Asadullah K. Cytokine and anti-cytokine therapy in dermatology. Expert Opinion on Biological Therapy. 2003. 3: 733-743. https://doi.org/10.1517/14712598.3.5.733
  18. Van Huis A. Edible insects are the future?. Proceedings of the Nutrition Society. 2016. 75: 294-305. https://doi.org/10.1017/S0029665116000069
  19. Van Itterbeeck J, van Huis A. Environmental manipulation for edible insect procurement: a historical perspective. Journal of Ethnobiology and Ethnomedicine. 2012. 8: 3. https://doi.org/10.1186/1746-4269-8-3
  20. Wicks IP, Roberts AW. Targeting GM-CSF in inflammatory diseases. Nature Reviews Rheumatology. 2016. 12: 37-48. https://doi.org/10.1038/nrrheum.2015.161
  21. Wong CK, Ho CY, Ko FW, Chan CH, Ho AS, Hui DS, Lam CW. Proinflammatory cytokines (IL-17, IL-6, IL-18 and IL-12) and Th cytokines (IFN-gamma, IL-4, IL-10 and IL-13) in patients with allergic asthma. Clinical and Experimental Immunology. 2001. 125:177-183. https://doi.org/10.1046/j.1365-2249.2001.01602.x
  22. Yoo YC, Shin BH, Hong JH, Lee J, Chee HY, Song KS, Lee KB. Isolation of fatty acids with anticancer activity from Protaetia brevitarsis larva. Archives of Pharmacal Research. 2007. 30: 361-365. https://doi.org/10.1007/BF02977619