대한의생명과학회지 (Biomedical Science Letters)

  • 제26권4호
  • /
  • Pages.275-287
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  • 2020
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지
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Validation of Reduced-volume Reaction in the PowerQuant® System for human DNA Quantification

  • Kim, Hyojeong (National Forensic Service, Forensic DNA Division) ;
  • Cho, Yoonjung (National Forensic Service, Forensic DNA Division) ;
  • Kim, Jeongyong (National Forensic Service, Forensic DNA Division) ;
  • Lee, Ja Hyun (National Forensic Service, Forensic DNA Division) ;
  • Kim, Hyo Sook (National Forensic Service, Forensic DNA Division) ;
  • Kim, Eungsoo (National Forensic Service Seoul Institute, DNA Analysis Division)
  • 투고 : 2020.11.20
  • 심사 : 2020.12.11
  • 발행 : 2020.12.31
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초록

Since its introduction in the forensic field, quantitative PCR (qPCR) has played an essential role in DNA analysis. Quality of DNA should be evaluated before short tandem repeat (STR) profiling to obtain reliable results and reduce unnecessary costs. To this end, various human DNA quantification kits have been developed. Among these kits, the PowerQunat® System was designed not only to determine the total amount of human DNA and human male DNA from a forensic evidence item, but also to offer data about degradation of DNA samples. However, a crucial limitation of the PowerQunat® System is its high cost. Therefore, to minimize the cost of DNA quantification, we evaluated kit performance using a reduced volume of reagents (1/2-volume) using DNA samples of varying types and concentrations. Our results demonstrated that the low-volume method has almost comparable performance to the manufacturer's method for human DNA quantification, human male DNA quantification, and DNA degradation index. Furthermore, using a reduced volume of regents, it is possible to run 2 times more reactions per kit. We expect the proposed low-volume method to cut costs in half for laboratories dealing with large numbers of DNA samples.

키워드

과제정보

Funded by National Forensic Service (NFS), Ministry of the Interior and Safety, Republic of Korea. Research grant: NFS2020DNA01.

참고문헌

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