Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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Antioxidant and Hepatoprotective Activities of Bitter Melon (Momordica charantia Linn.) Leaves against Oxidative Stress

산화적 스트레스에 대한 여주(Momordica charantia Linn.)잎의 항산화 활성 및 간세포 보호능

  • Jeon, Ahyeong (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Cheon, Wonyoung (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Yoon, Jimin (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Kim, Dae-Jung (Experiment research institute, National Agricultural Products Quality Management Service) ;
  • Kim, Younghwa (School of Food Biotechnology and Nutrition, Kyungsung University)
  • 전아영 (경성대학교 식품생명공학과 대학원) ;
  • 천원영 (경성대학교 식품생명공학과 대학원) ;
  • 윤지민 (경성대학교 식품생명공학과 대학원) ;
  • 김대중 (국립농산물품질관리원 시험연구소) ;
  • 김영화 (경성대학교 식품응용공학부)
  • Received : 2020.11.20
  • Accepted : 2020.12.15
  • Published : 2020.12.31
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Abstract

This study examined the bioactive compound content and the antioxidant activities of bitter melon (Momordica charantia Linn.) leaves. The content of vitamin C, beta-carotene, and total carotenoids was 69.77, 45.68, and 65.08 mg/100 g, respectively. To investigate the antioxidant capacity, bitter melon leaves were extracted using various concentrations of ethanol (60, 80, or 100%). Highest content of total polyphenols (18.07 mg gallic acid equivalent/g) and flavonoids (4.53 mg cathechin equivalent/g) was found in the 100% ethanolic extract of the leaves (E100). Also, the E100 extract showed the highest levels of 2,2'azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities. Reducing power was also the highest (39.21 mg Trolox equivalent/g) in E100 extract. The E100 extract effectively inhibited lipid peroxidation by 91.45% compared to the control group. Also, the E100 extract showed a cytoprotective effect against oxidative stress in HepG2 cells and decreased the generation of intracellular reactive oxygen species. These results suggest that bitter melon leaves could be regarded as a potential source of natural antioxidants.

Keywords

References

  1. Ahn MJ, Yuk HJ, Lee HY, Hwang CE, Jeong YS, Hong SY, et al. 2015. Effect of the enhanced biological activities and reduced bitter taste of bitter melon (Momordica charantia L.) by roasting. J Agric Life Sci., 49:107-119 https://doi.org/10.14397/jals.2015.49.2.107
  2. AOAC. 2016. Official methods of analysis of the AOAC. Association of Official Agricultural Chemists International, Gaithersburg, MD, USA, chapter 45, pp 9-10
  3. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature, 181:1199-1200 https://doi.org/10.1038/1811199a0
  4. Boo HO, Lee HH, Lee JW, Hwang SJ, Park SU. 2009. Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean J Med Crop Sci., 17:15-20
  5. Cha JY, Kim YT, Kim HS, Cho YS. 2008. Antihyperglycemic effect of stem bark powder from paper mulberry (Broussonetia kazinoki Sieb.) in type 2 diabetic Otsuka Long-Evans Tokushima fatty rats. J Med Food., 11:499-505 https://doi.org/10.1089/jmf.2007.0028
  6. Cha MN, Oh MS. 1996. Changes in mineral content in several leaf vegetables by various cooking methods. Korean J Soc Food Sci., 12(1):34
  7. Cheon W, Seo D, Kin Y. 2019. Antioxidative and hepatocyte protective effects of guava (Psidium guajava L.) leaves cultivated in Korea. Korean J Food & Nutr., 32(1):33-40 https://doi.org/10.9799/KSFAN.2019.32.1.033
  8. Choi JR, Choi JM, Lee S, Cho KM, Cho EJ, Kim HY. 2014. The protective effects of protocatechuic acid from Momordica charantia against oxidative stress in neuronal cells. Korean J Pharmacogn., 45:11-16
  9. Choi YH. 2003. Changes in vitamin C and minerals content of Perilla leaves by different cooking methods. Korean J Soc Food Cookery Sci., 19(2):174-180
  10. Dragsted LO. 2003. Antioxidant actions of polyphenols in humans. Int J Vitam Nutr Res., 73:112-119 https://doi.org/10.1024/0300-9831.73.2.112
  11. Emerit J, Chaudiere J. 2008. Free radicals and lipid peroxidation in cell pathology. In handbook of free radicals and antioxidants in biomedicine. CRC Press. pp 177-185
  12. Folin O, Denis W. 1912. On phosphotungstic-phosohomolybdic compounds as colour reagents. J Biol Chem., 12:239-249 https://doi.org/10.1016/S0021-9258(18)88697-5
  13. Gomez R, Campos DA, Oliveira A, Aguilar CN, Madureira AR, Pintado M. 2020. A chemical valorisation of melon peels towards functional food ingredients: Bioactives profile and antioxidant properties. Food Chem., 335:127-579
  14. Grover JK, SP Yadav. 2004. Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharm., 93:123-132 https://doi.org/10.1016/j.jep.2004.03.035
  15. Halliwell B, Gutteridge JM. 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol., 186:1-85 https://doi.org/10.1016/0076-6879(90)86093-B
  16. Holick CN, Michaud DS, Stolzenberg-Solomon R, Mayne ST, Pietinen P, Taylor PR, Virtamo J, Albanes D. 2002. Dietary carotenoids, serum beta-carotene, and retinol and risk of lung cancer in the alpha-tocopherol, betacarotene cohort study. Am J Epidemiol., 156:536-547 https://doi.org/10.1093/aje/kwf072
  17. Huang MT, Osawa T, HO T, Rosen RT. 1994. Food phytochemicals for cancer prevention. J Am Chem Soc., 50:71-73
  18. Huang WC, Tsai TH, Huang CJ, Li YY, Chyuan JH, Chuang LT, Tsai PJ. 2015. Inhibitory effects of wild bitter melon leaf extract on Propionbacterium aces-induced skin inflammation in mouse and cyrokine production in vitro. Food & Func., 162:1-42
  19. Hurrle S, Hsu WH. 2017. The etiology of oxidative stress in insulin resistance. Biomed J., 40:257-262 https://doi.org/10.1016/j.bj.2017.06.007
  20. Hwang ES. 2018. Comparison of antioxidant capacity and α-glucosidase inhibitory activity between bitter melon (Momordica charanti) fruit and leaf extract. Asian Pac J Trop Biomed., 8:189-193 https://doi.org/10.4103/2221-1691.231280
  21. Kang JR, Kang MJ, Kim GW, Shin JH. 2018. Physicochemical characteristics and physiological activity of Bitter Melon (Momordica charantia L.) from different regions. Korean J Food & Nutr., 47:253-262 https://doi.org/10.3746/jkfn.2018.47.3.253
  22. Kang YH, Park YK, Oh SR, Moon KD. 1995. Studies on the physiological functionality of pine needle and mugwort extracts. Korean J Food & Nutr., 27:978-984
  23. Karaman K, Dalda-Sekerci A, Yetisir H, Gulsen O, Coskun OF. 2018. Molecular, morphological and biochemical characterization of son Turkish bitter melon (Momordica charantia L.) genotypes. Ind Crops Prod., 123:93-99 https://doi.org/10.1016/j.indcrop.2018.06.036
  24. Kim HJ, Seleshe S, Ameer A, Kim BJ, Park JG, Kang SN. 2020. Antioxidant and antimicrobial activity in oat (Avena sativa L.) leaf extracts and its effect on the characteristics of emulsion sausage. J Agric Life Sci., 54(2):83-92
  25. Kim YS, Hwang JW, Park PJ, Jeong JH. 2014. Antioxidant activity and protective effects of extracts from Chrysanthemum boreale on t-BHP induced oxidative stress in Chang cells. J Korean Soc Food Sci Nutr., 43(1):60-66 https://doi.org/10.3746/JKFN.2014.43.1.060
  26. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeon BT. 2011a. Antioxidant activity and protective effects of extracts from Helianthus tuberosus L. leaves on t-BHP induced oxidative stress in Chang Cells. Korean J Food & Nutr., 40(11):1525-1531 https://doi.org/10.3746/jkfn.2011.40.11.1525
  27. Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeon BT. 2011b. Antioxidant activities of extracts from Ligustrum ovalifolium H. leaves. J Korean Soc Food Sci Nutr., 40(12):1642-1647 https://doi.org/10.3746/JKFN.2011.40.12.1642
  28. Kwon DK, Son YJ, Park H. 2019. The effect of acetic acid fermented onion vinegar supplementation and acute swimming exercise on lipid peroxidation and antioxidant enzyme activity of rats fed a high-fat diet. Korean J Sports Med Acronym., 28:875-884 https://doi.org/10.35159/kjss.2019.06.28.3.875
  29. Lee HJ, Lee BJ, Lee DS, Seo YW. 2003. DPPH radical scavenging effect and in vitro lipid peroxidation inhibition by Portulaca oleracea. Korean J Soc Biotechnol Bioeng., 18:165-169
  30. Lee KM, Kwon TY, Kang U, Seo EK, Yun JH, Nho CW, Kim YS. 2017. Tussilagonone-induced Nrf2 pathway acitivation protects HepG2 cells from oxidative injury. Food Chem Toxicol., 108:120-127 https://doi.org/10.1016/j.fct.2017.07.035
  31. Lee OH, Lee HB, Lee J, Son JY, Rhee SK, Kim HD, Kim YC, Lee BY. 2005. Chemical properties of olive and bay leaves. J Korean Soc Food Sci Nutr., 34(4):503-508 https://doi.org/10.3746/JKFN.2005.34.4.503
  32. Lee SN. 2014. The antioxidant effect of rutin in human dermal fibroblasts damaged by reactive oxygen species. Korean J Aesthet Cosmetol., 12(6):831-836
  33. Lee YS. 2007. Physiological activities of ethanol extracts from different parts of Ailanthus altissima. J Korean Soc Food Sci Nutr., 36:389-394 https://doi.org/10.3746/JKFN.2007.36.4.389
  34. Leibovitz BE, Siegel BV. 1980. Aspects of free radical reactions in biological systems: aging. J Gerontol., 35:45-56 https://doi.org/10.1093/geronj/35.1.45
  35. Limtrakul P, Khantamat O, Pintha K. 2004. Inhibition of Pglycoprotein activity and reversal of cancer multidrug resistance by Momordica charantia extract. Cancer Chemother Pharmacol., 54:525-530 https://doi.org/10.1007/s00280-004-0848-4
  36. Oh DY, Kim HS. 2019. Evaluation of oxidation inhibition and nitrogen oxide scavenging activity from Curcuma longa L. extracts. J Korean Appl Sci Technol., 36(1):13-22 https://doi.org/10.12925/JKOCS.2019.36.1.13
  37. Oyaizu M. 1986. Studies on products of browning reaction: antioxidant activities of products of browning reaction prepared from glucoseamine. Jpn J Nutr Diet., 44:307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  38. Perron NR, Brumaghim JL. 2009. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys., 53:75-100 https://doi.org/10.1007/s12013-009-9043-x
  39. Phillips KM, Tarrago-Trani MT, Gebhardt SE, Exler J, Patterson KY, Haytowitz DB, Pehrsson PR, Holden JM. 2010. Stability of vitamin C in frozen raw fruit and vegetable homogenates. J Food Compos Anal., 23:253-259 https://doi.org/10.1016/j.jfca.2009.08.018
  40. Reddy BS, Reddy RKK, Reddy BP, Ramakrishna S, Diwan PV. 2008. Potential in vitro antioxidant and protective effects of Soymida febrifuga on ethanol induced oxidative damage in HepG2 cells. Food Chem Toxical., 46:3429-3442 https://doi.org/10.1016/j.fct.2008.08.034
  41. Rhim TJ, Lim SC. 2005. The hepatotprotective and antioxidative effects of onion (Allium cepa) extracts in rat hepatocyte primary culture. Korean J. Plant Res., 18(1):52-60
  42. Siriwardhana N, Lee KW, Jeon YJ, Kim SH, Haw JW. 2003. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Technol Int., 9:339-346 https://doi.org/10.1177/1082013203039014
  43. Yeon SH, Ham H, Sung J, Kim Y, Namkoong S, Jeong HS, Lee JS. 2013. Antioxidant activities of hot water extract from Cornus walteri Wanger against oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells. J Korean Soc Food Sci Nutr., 42:1525-1532 https://doi.org/10.3746/JKFN.2013.42.10.1525
  44. Zhang M, Hettiarachchy NS, Horax R, Chen P, Over KF. 2009. Effect of maturity stages and drying methods on the retention of selected nutrients and phytochemicals in bitter melon (Momordica charantia) leaf. J Food Sci., 74:C441-C448 https://doi.org/10.1111/j.1750-3841.2009.01222.x
  45. Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  46. Food safety Korea. 2020. Available from: http://www.foodsafetykorea.go.kr/portal/safefoodlife/foodMeterial/foodMeterialDB.do, [aceesed 2020. Oct. 03]