DOI QR코드

DOI QR Code

Crosslinking Effects and Mechanical Properties of Glutaraldehyde Crosslinked Wet-Spun Collagen Fibers

글루타르알데히드 가교 조건에 따른 콜라겐 섬유의 방사성 및 역학적 특성 분석

  • 안현철 (한국생산기술연구원 섬유융합연구부문) ;
  • 공다정 (한국생산기술연구원 섬유융합연구부문) ;
  • 이현호 (파이버엔텍) ;
  • 서주연 (파이버엔텍) ;
  • 김태겸 (파이버엔텍) ;
  • 이소희 (경상대학교 의류학과) ;
  • 여상영 (한국생산기술연구원 섬유융합연구부문)
  • Received : 2020.11.23
  • Accepted : 2020.12.21
  • Published : 2020.12.31

Abstract

Collagen is a natural protein with excellent biocompatibility. Purified collagen is used in various medical and cosmetic products. Fibers made from collagen are particularly used for medical purposes, but their mechanical properties are not good; therefore, manufacturing processes such as crosslinking and blending are being researched and developed to reinforce fibers and improve fiber spinnability. Particularly, in the crosslinking process, fiber properties can be manipulated and adjusted based on the crosslinking agent. In this study, the spinnability and mechanical properties of the fibers were analyzed according to the crosslinking concentration and crosslinking process time. Through this, the crosslinked collagen spinning conditions that can achieve a sufficient crosslinking effect with a minimum concentration and process time were examined. The processing efficiency and mechanical properties of the collagen fibers were improved by optimizing the crosslinking conditions.

Keywords

Acknowledgement

이 논문은 산업통상자원부의 지원을 받아 수행된 연구임(과제번호 20000519).

References

  1. A. Gautieri, S. Vesentini, A. Redaelli, and M. J. Buehler, "Hierarchical Structure and Nanomechanics of Collagen Microfibrils from the Atomistic Scale Up", Nano Lett., 2011, 11, 757-766. https://doi.org/10.1021/nl103943u
  2. Z. Bazrafshan and G. K. Stylios, "Spinnability of Collagen as a Biomimetic Material: A Review", Int. J. Biol. Macromol., 2019, 129, 693-705. https://doi.org/10.1016/j.ijbiomac.2019.02.024
  3. L. Yang, C. F. C. Fitie, K. O. van der Werf, M. L. Bennink, P. J. Dijkstra, and J. Feijen, "Mechanical Properties of Single Electrospun Collagen Type I Fibers", Biomaterials, 2008, 29, 955-962. https://doi.org/10.1016/j.biomaterials.2007.10.058
  4. L. Yang, K. O. van der Werf, P. J. Dijkstra, J. Feijen, and M. L. Bennink, "Micromechanical Analysis of Native and Cross-linked Collagen Type I Fibrils Supports the Existence of Microfibrils", J Mech. Behav. Biomed., 2012, 6, 148-158. https://doi.org/10.1016/j.jmbbm.2011.11.008
  5. M. Meyer, "Processing of Collagen Based Biomaterials and the Resulting Materials Properties", Biomed. Eng. Online, 2019, 18, 24.
  6. S. Akhshabi, E. Biazar, V. Singh, S. Heidari Keshel, and G. Nagaraja, "The Effect of Glutaraldehyde Cross-linker on Structural and Biocompatibility Properties of Collagen-chondroitin Sulfate Electrospun Mat", Mater. Technol., 2018, 33, 253-261. https://doi.org/10.1080/10667857.2017.1410998
  7. L. H. H. Olde Damink, P. J. Dijkstra, M. J. A. Van Luyn, P. B. Van Wachem, P. Nieuwenhuis, and J. Feijen, "Glutaraldehyde as a Crosslinking Agent for Collagen-based Biomaterials", J. Mater. Sci.-Mater. M., 1995, 6, 460-472. https://doi.org/10.1007/BF00123371
  8. N. Davidenko, C. F. Schuster, D. V. Bax, N. Raynal, R. W. Farndale, S. M. Best, and R. E. Cameron, "Control of Crosslinking for Tailoring Collagen-based Scaffolds Stability and Mechanics", Acta Biomater., 2015, 25, 131-142. https://doi.org/10.1016/j.actbio.2015.07.034
  9. D. T. Cheung and M. E. Nimni, "Mechanism of Crosslinking of Proteins by Glutaraldehyde II. Reaction with Monomeric and Polymeric Collagen", Connect. Tissue Res., 1982, 10, 201-216. https://doi.org/10.3109/03008208209034419
  10. D. T. Cheung and M. E. Nimni, "Mechanism of Crosslinking of Proteins by Glutaraldehyde II. Reaction with Monomeric and Polymeric Collagen", Connect. Tissue Res., 1982, 10, 201-216. https://doi.org/10.3109/03008208209034419
  11. G. Tronci, R. S. Kanuparti, M. T. Arafat, J. Yin, D. J. Wood, and S. J. Russell, "Wet-spinnability and Crosslinked Fibre Properties of Two Collagen Polypeptides with Varied Molecular Weight", Int. J. Biol. Macromol., 2015, 81, 112-120. https://doi.org/10.1016/j.ijbiomac.2015.07.053
  12. G. P. Huang, S. Shanmugasundaram, P. Masih, D. Pandya, S. Amara, G. Collins, and T. L. Arinzeh, "An Investigation of Common Crosslinking Agents on the Stability of Electrospun Collagen Scaffolds", J. Biomed. Mater. Res. A., 2015, 103, 762-771. https://doi.org/10.1002/jbm.a.35222
  13. R. Tonndorf, E. Gossla, D. Aibibu, M. Lindner, M. Gelinsky, and C. Cherif, "Corrigendum: Wet Spinning and Riboflavin Crosslinking of Collagen Type I/III Filaments (2019 Biomed. Mater. 14 015007)", Biomed. Mater., 2019, 14, 039501. https://doi.org/10.1088/1748-605x/ab0870
  14. R. Tu, S.-H. Shen, D. Lin, C. Hata, K. Thyagarajan, Y. Noishiki, and R. C. Quijano, "Fixation of Bioprosthetic Tissues with Monofunctional and Multifunctional Polyepoxy Compounds", J Biomed. Mater. Res., 1994, 28, 677-684. https://doi.org/10.1002/jbm.820280604
  15. J. Zhan, Y. Morsi, H. Ei-Hamshary, S. S. Al-Deyab, and X. Mo, "In vitro Evaluation of Electrospun Gelatin-glutaraldehyde Nanofibers", Front. Mater. Sci., 2016, 10, 90-100. https://doi.org/10.1007/s11706-016-0329-9
  16. J. Kim, J. Yu, D. Seo, and J. Jang, "Medical Materials Produced Using Collagen and Method for Manufacturing the Same", KR Patent, 101876196 (2017).
  17. M. T. Arafat, G. Tronci, J. Yin, D. J. Wood, and S. J. Russell, "Biomimetic Wet-stable Fibres via Wet Spinning and Diacid-based Crosslinking of Collagen Triple Helices", Polymer, 2015, 77, 102-112. https://doi.org/10.1016/j.polymer.2015.09.037
  18. J. Jang, H. Jeong, J. Yu, S. Yeo, and D. Seo, and J. Jang, "Method for Producing High-concentration Collagen for Using as Medical Material", KR Patent, 101531479 (2014).