Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Inhibitory Effect of Polyporus umbellatus Extract on Melanogenesis

저령 추출물의 멜라닌 생성억제 작용

  • Kang, Lea Minju (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park, Seol-a (Department of Beauty Design, Graduate School, Wonkwang University) ;
  • Mun, Yeun-Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo, Won-Hong (Research Center of Environmental Science, Wonkwang University)
  • 강리아민주 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 박설아 (원광대학교 일반대학원 뷰티디자인학과) ;
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 환경과학연구소)
  • Received : 2020.02.17
  • Accepted : 2020.03.10
  • Published : 2020.03.27
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Abstract

Objectives : The purpose of this study was to investigate melanogenesis inhibition of ethanol extract of Polyporus (EP) by using B16F10 melanoma cells. Methods : We measured antioxidant effect of EP by using 1,1-Diphenyl-1-picrylhydrazyl (DPPH) assay and we confirmed melanin contents and tyrosinase activity of EP in cells. Additionally, the expression of tyrosinase-related protein-1 (TRP-1) and TRP-2 was observed by Western blot. Results : EP showed significantly high radical scavenging activity and inhibition of melanogenesis in dose-dependent manner by decreasing cellular tyrosinase activity and melanin content with or without α-melanin stimulating hormone. TRP-1 and TRP-2 expressions were also suppressed by EP in B16F10 cells. Conclusions : These results suggest that EP inhibits the melanogenesis and it could be a new organic ingredient for hyper-pigmentation.

Keywords

References

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