Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

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Effects of Mitochondria-targeted Antioxidant MitoTEMPO on the Kinetic Characteristics of Frozen-Thawed Boar Sperm

동결-융해 정자의 운동학적 특성에 대한 MitoTEMPO의 영향

  • Cho, Eun Seok (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Jeong A (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong, Yong Dae (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Choi, Yo Han (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Hong, Jun Ki (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Young Sin (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Chung, Hak Jae (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Baek, Sun Young (Swine Science Division, National Institute of Animal Science, Rural Development Administration) ;
  • Sa, Soo Jin (Swine Science Division, National Institute of Animal Science, Rural Development Administration)
  • 조은석 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김정아 (농촌진흥청 국립축산과학원 양돈과) ;
  • 정용대 (농촌진흥청 국립축산과학원 양돈과) ;
  • 최요한 (농촌진흥청 국립축산과학원 양돈과) ;
  • 홍준기 (농촌진흥청 국립축산과학원 양돈과) ;
  • 김영신 (농촌진흥청 국립축산과학원 양돈과) ;
  • 정학재 (농촌진흥청 국립축산과학원 양돈과) ;
  • 백선영 (농촌진흥청 국립축산과학원 양돈과) ;
  • 사수진 (농촌진흥청 국립축산과학원 양돈과)
  • Received : 2020.01.28
  • Accepted : 2020.03.06
  • Published : 2020.03.31
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Abstract

Cryopreservation of semen is useful for animal breeding via artificial insemination (AI). However, the use of frozen-thawed boar semen is limited due to cryodamage. The aim of this study was to investigate the effects of different concentrations of MitoTEMPO (a mitochondria-targeted antioxidant) in lactose-egg yolk (LEY) extenders on kinetic characteristics of frozen-thawed boar sperms. Semen samples were collected from mature Duroc boars (2~3 years old) and cryopreserved in LEY extenders containing 0, 0.5, 5, 50, and 500 μM MitoTEMPO. The kinetic characteristics of frozen-thawed sperms were determined 0 and 30 min after thawing using computer-assisted sperm analysis (CASA). Results indicated that sperm motility immediately after thawing was significantly higher with 5 and 50 μM (50.46±2.71% and 46.96±2.66%, respectively) than with 500 μM MitoTEMPO (35.40±2.95%) (P<0.05). However, there were no significant differences in other kinetic characteristics except motility. In conclusion, the addition of MitoTEMPO to the sperm freezing extender may have a beneficial effect on motility of post-thawed boar semen.

정액의 동결보존은 인공수정을 통한 동물 번식에 유용한 것으로 알려져 있지만 동결-융해된 돼지 정액의 사용은 저온손상 때문에 제한된다. 최근에는 이를 보완하기 위해 다양한 첨가제 연구가 진행 되고 있다. 그 중 항산화제는 정자의 동결 융해과정에서 정자 세포막의 지질과산화를 억제시켜 정자의 생존성과 운동성을 개선시킨 다고 알려져 있다. 본 연구의 목적은 동결보존액에 대한 MitoTEMPO(미토콘드리아 표적 항산화제) 첨가가 돼지 동결-융해 정자의 운동학적 특성에 미치는 영향을 평가하는 것이다. 성숙한 Duroc종 수퇘지로부터 정액샘플을 채취하였으며, 다양한 농도의 MitoTEMPO (0, 0.5, 5, 50 및 500 μM)를 lactose-egg yolk 동결보존액에 첨가하여 정액을 동결하였다. 동결-융해 후 정자의 운동학적 특성들은 정자자동분석기 (CASA; computer-assisted sperm analysis)를 이용하여 분석하였다. 그 결과, 동결용 보존액에 5 및 50 μM (50.46±2.71%, 46.96±2.66%) MitoTEMPO 첨가 시 500 μM 처리구(35.40±2.95%)에 비해 유의적으로 높은 정자 운동성을 나타냈다(P<0.05). 그렇지만, 운동성을 제외한 다른 운동학적 특성에서는 유의적인 차이를 보이지 않았다. 결론적으로 동결용 보존액에 대한 MitoTEMPO 첨가는 동결-융해 돼지 정자의 운동성에 긍정적인 영향을 미치는 것으로 사료된다.

Keywords

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