Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
DOI QR코드

DOI QR Code

Pharmacopuncture of Bauhinia variegata Nanoemulsion Formulation against Diabetic Peripheral Neuropathic Pain

  • Gupta, Pushpraj S (Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture) ;
  • Singh, Sunil K (Department of Pharmaceutical Sciences, United Institute of Pharmacy) ;
  • Tripathi, Abhishek K (Department of Pharmaceutical Sciences, Sam Higginbottom University of Agriculture)
  • Received : 2019.07.02
  • Accepted : 2019.12.02
  • Published : 2020.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The objective of the study was to prepare Bauhinia variegata loaded nanoemulsion(formulation and determine the efficacy of herbal drug formulation against diabetic peripheral neuropathic pain through acupuncture technique. Methods: Nine different ba tches of nanoemulsion (NE1 NE9) of BVN was prepared by varying the Smix ratio and the concentration of oil. BVN was characterized to determine particle size, shape, zeta potential, polydispersity index, optical transmittance, drug release profile and stora ge stability. The optimized formulation was subjected to plantar test, behavioral tests of neuropathic pain and Von Frey filament stimulation test. Diabetes was induced by intraperitoneal injection of freshly prepared solution of Streptozotocin (60 mg/kg) to the experimental rats. Animals were made diabetic divided into four groups, Group I was untreated normal control group, Group II was diabetic control group, Group III was Bauhinia variegata extract ( treated group (100 mg/kg/day, p.o) and Group IV was BVN treated groups (100 mg/kg/day, p.o) acute and chronically. Results: The prepared B. variegata loaded nanoemulsion was nanosized (124 nm), spherical, uniform and stable over the period of 180 days with no change in physiochemical properties. The bl ood glucose and body weight of animals was normalizing after four weeks of treatment that was significant with BVN in comparison to diabetic control group. The chronic administration of BVN significantly (P<0.001) decreased hind paw withdrawal latency an d attenuated mechanical allodynia as compared with diabetic rats. Conclusion: Thus, BVN may be an effective drug formulation against diabetic peripheral neuropathic pain.

Keywords

References

  1. Fangueiro JF, Silva AM, Garcia ML, Souto EB. European Journal of Pharmaceutics and Biopharmaceutics Current nanotechnology approaches for the treatment and management of diabetic retinopathy. Eur J Pharm Biopharm. 2015;95:307-22. https://doi.org/10.1016/j.ejpb.2014.12.023
  2. Kesharwani P, Gorain B, Low SY, Tan SA, Ling EC, Lim YK, et al. Nanotechnology based approaches for anti-diabetic drugs delivery. Diabetes Res Clin Pract. 2017;136:52-77. https://doi.org/10.1016/j.diabres.2017.11.018
  3. Landon MB, Spong CY, Thom E, Carpenter MW, Ramin SM, Casey B, et al. Eunice Kennedy Shriver National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network, A multicenter, randomized trial of treatment for mild gestational diabetes. N Engl J Med. 2009;361:1339-48. https://doi.org/10.1056/NEJMoa0902430
  4. Ziegler D. Painful diabetic neuropathy: treatment and future aspects. Diabetes Metab Res Rev. 2008;24(1):S52 - S57. https://doi.org/10.1002/dmrr.817
  5. Teeranachaideekul V, Boonme P, Souto EB, Muller RH, Junyaprasert VB. Influence of oil content on physicochemical properties and skin distribution of nile red-loaded NLC. J Control Release. 2008;128(2):134-41 https://doi.org/10.1016/j.jconrel.2008.02.011
  6. Sayago CT, de Camargo VB, Barbosa F, Gularte C, Pereira G, Miotto S, et al. Chemical composition and in vitro antioxidant activity of hydro-ethanolic extracts from Bauhinia forficata subsp. pruinosa and B. variegata. Acta Biol Hung. 2013;64(1):21-33. https://doi.org/10.1556/ABiol.64.2013.1.3
  7. Filho CV. Chemical composition and biological potential of plants from the genus Bauhinia. Phytotherapy Research. 2009;23(10):1347-54 https://doi.org/10.1002/ptr.2756
  8. Ahmed AS, Elgorashi EE, Moodley N, McGraw LJ, Naidoo V, Eloff JN. The antimicrobial, antioxidative, anti-inflammatory activity and cytotoxicity of different fractions of four South African Bauhinia species used traditionally to treat diarrhea. J. Ethnopharmacol. 2012;143:826-39. https://doi.org/10.1016/j.jep.2012.08.004
  9. Mohamed MA, Mammoud MR, Hayen H. Evaluation of antinociceptive and anti-inflammatory activities of a new triterpene saponin from Bauhinia variegata leaves. Z. Naturforsch. 2009;64C:798-808. https://doi.org/10.1515/znc-2009-11-1208
  10. Rachmawati H, Budiputra DK, Suhandono S, Anggadiredga K. Curcumin nanoemulsion for transdermal application: formulation and evaluation. Research and development on nanotechnology in Indonesia. 2014;1(1);5-8.
  11. Amudha P, Komala M. Formulation and in-vitro evaluation of self nanoemulsion containing Eclipta alba extract. IJBPR. 2014;5(11):882-5.
  12. Zainol NA, Ming TS, Darwis Y. Development and characterization of cinnamon leaf oil nanocream for topical application. Indian Journal of Pharmaceutical Sciences. 2015:422-33
  13. Banafshe HR, Hamidi G a., Noureddini M, Mirhashemi SM, Mokhtari R, Shoferpour M. Effect of curcumin on diabetic peripheral neuropathic pain: Possible involvement of opioid system. Eur J Pharmacol. 2014;723(1):202-6. https://doi.org/10.1016/j.ejphar.2013.11.033
  14. Montagne-Clavel J, Oliveras JL. The plantar test apparatus (Ugo Basile Biological Apparatus), a controlled infrared noxious radiant heat stimulus for precise withdrawal latency measurement in the rat, as a tool for humans? Somatosens Mot Res. 1996;13(3-4):215-23. https://doi.org/10.3109/08990229609052577
  15. Chaplan SR, Bach FW, Pogrel JW, Chung JM, Yaksh TL. Quantitative assessment of tactile allodynia in the rat paw. J. Neurosci. Methods. 1994;53(1):55-63. https://doi.org/10.1016/0165-0270(94)90144-9
  16. Rathore K, Singh VK, Jain P, Rao SP, Ahmed Z, Singh VD. In-vitro and in-vivo antiadipogenic, hypolipidemic and antidiabetic activity of Diospyros melanoxylon (Roxb). J Ethnopharmacol [Internet]. 2014;155(2):1171-1176. https://doi.org/10.1016/j.jep.2014.06.050
  17. Kumar V, Rathore K, Jain P, Ahmed Z. Biological activity of Bauhinia racemosa against Diabetes and Interlinked Disorders like Obesity and Hyperlipidemia. Clin Phytoscience. 2017;3(1). https://doi.org/10.1186/s40816-017-0052-9
  18. Singh P, jain P, shukla S, pandey R. Phytotherapeutic review on diabetes. Spat DD - Peer Rev J Complement Med Drug Discov. 2016;6(2):1.
  19. Bansal V, Kalita J, Misra UK. Diabetic neuropathy. Postgrad Med J. 2006;82(964):95-100. https://doi.org/10.1136/pgmj.2005.036137
  20. Raz I, Hasdi D, Seltzer, Melmed RN. Effect of hyperglycemia on pain perception and on efficacy of morphine analgesia in rats. Diabetes. 1988;37:1253-9. https://doi.org/10.2337/diabetes.37.9.1253
  21. Ochoa JL. Pain mechanism in neuropathy. Curr Opin Neurol. 1994;7:407-14 https://doi.org/10.1097/00019052-199410000-00008
  22. Mark AR. Neuropathies associated with diabetes. Med Clin North Am. 1993;27:111-24.
  23. Kumar V, Jain P, Rathore K, Ahmed Z. Biological Evaluation of Pupalia lappacea for Antidiabetic, Antiadipogenic, and Hypolipidemic Activity Both In Vitro and In Vivo. Scientifica (Cairo). 2016;2016.
  24. Malcangio M, Tomlinson DR. A pharmacologic analysis of mechanical hyperalgesia in streptozotocin/diabetic rats. Pain.1998;76:151-7. https://doi.org/10.1016/S0304-3959(98)00037-2
  25. Calcutt NA. Experimental models of painful diabetic neuropathy. J. Neurol. Sci. 2004;220(1-2): 137-9. https://doi.org/10.1016/j.jns.2004.03.015
  26. Simon GS, Dewey WL. Effect of streptozotocin induced diabeties on the antinociceptive potency of Morphine. J Pharmacol Exp Ther. 1981;218:318-23.
  27. Tembhurne SV, Sakarkar DM. Effect of fluoxetine on an experimental model of diabetes-induced neuropathic pain perception in the rat. Indian J Pharm Sci. 2011;73(6):621-5. https://doi.org/10.4103/0250-474X.100235
  28. Dubin AE, Patapoutian A. Nociceptors: the sensors of the pain pathway. J. Clin. Invest. 2010;120(11):3760-72. https://doi.org/10.1172/JCI42843
  29. Liu H, Yuan H, Wu B, Gao Y, Liu S, Shi L, et al. Nanoparticle-Encapsulated Curcumin Inhibits Diabetic Neuropathic Pain Involving the P2Y12 Receptor in the Dorsal Root Ganglia. Front Neurosci. 2018;11:1-12.
  30. Hosseini A, Sharifzadeh M, Rezayat SM, Hassanzadeh G, Hassani S, Baeeri M, et al. Benefit of magnesium-25 carrying porphyrin-fullerene nanoparticles in experimental diabetic neuropathy. International Journal of Nanomedicine, 2010;5(1):517-23.