Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitiory Effects of Mixture of Atractylodes Macrocephala and Amomum Villosum Extracts on Adipocyte Differentiation in OP9 Cells

백출과 사인 추출 혼합물의 지방세포분화 억제 효과

  • Kim, Ha Rim (Department of Physiology, School of Korean Medicine, Wonkwang University) ;
  • Kwon, Yong Kwan (Department of Physiology, School of Korean Medicine, Wonkwang University) ;
  • Choi, Bong Keun (NutraPharm Tech Co., Ltd.) ;
  • Baek, Dong Gi (Department of Korean Internal Medicine, Iksan Korean Medicine Hospital, Wonkwang University)
  • 김하림 (원광대학교 한의과대학 생리학교실) ;
  • 권용관 (원광대학교 한의과대학 생리학교실) ;
  • 최봉근 ((주)뉴트라팜텍) ;
  • 백동기 (원광대학교 한의과대학 익산한방병원 한방내과)
  • Received : 2019.02.05
  • Accepted : 2020.02.25
  • Published : 2020.02.25
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Abstract

In this study, we investigated the inhibition effects of mixtures of Atractylodes macrocephala (AM) and Amomum villosum (AV) water extracts on adipocyte differentiation. Treatment with mixtures of AM and AV extracts in a ratio of 3:1 for 24 and 48 hours did not show any cytotoxicity in OP9 cells. Mixtures of AM(3) and AV(1) extracts inhibited adipocyte differentiation, expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAT/enhancer-binding protein alpha (C/EBPα), the major transcription factors of differentiation. It also inhibited the expression of lipoprotein lipase (LPL), adipocyte protein 2 (aP2), which are PPARγ-target genes in adipocyte. We also checked the inhibition effects on cell proliferation during the early stage of differentiation by treatment with mixtures of AM(3) and AV(1) extracts. It markedly inhibited adipocyte proliferation after 48 hours, and also the phosphorylation of ERK1/2 and Akt after 10 min or 3 hour. These results identify a possible mechanism of action of mixtures of AM(3) and AV(1) extracts, suggesting that the mixtures of AM(3) and AV(1) extracts-induced inhibition of ERK and Akt phosphorylation suppresses adipogenesis by inhibiting other signaling cascades that include PPARγ and C/EBPα during the process of OP9 adipocyte differentiation.

Keywords

References

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