Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Seroprevalence of Sarcocystis falcatula in Two Islands of Malaysia using Recombinant Surface Antigen 4

  • Received : 2019.07.29
  • Accepted : 2019.11.06
  • Published : 2020.02.29
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Abstract

Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis.

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References

  1. Fayer R, Esposito DH, Dubey JP. Human Infections with Sarcocystis Species. Clinl Microbiol Rev 2015; 28: 295-311. https://doi.org/10.1128/CMR.00113-14
  2. Wong KT, Pathmanathan R. High prevalence of human skeletal muscle sarcocystosis in south-east Asia. Trans R Soc Trop Med Hyg 1992; 86: 631-632. https://doi.org/10.1016/0035-9203(92)90161-5
  3. Mandour AM. Sarcocystis nesbitti n. sp. from the rhesus monkey. J Protozool 1969; 16: 353-354. https://doi.org/10.1111/j.1550-7408.1969.tb02281.x
  4. Yang ZQ, Wei CG, Zen JS, Song JL, Zuo YX, He YS, Zhang HF, Attwood SW, Chen XW, Yang GC, Zhou X, Quan X, Li CY, Han D, Liu AW, Lim P. A taxonomic re-appraisal of Sarcocystis nesbitti (Protozoa: Sarcocystidae) from the monkey Macaca fascicularis in Yunnan, PR China. Parasitol Int 2005; 54: 75-81. https://doi.org/10.1016/j.parint.2004.12.004
  5. Lau YL, Chang PY, Subramaniam V, Ng YH, Mahmud R, Ahmad A, Fong MY. Genetic assemblage of Sarcocystis spp. in Malaysian snakes. Parasit Vectors 2013; 6: 257. https://doi.org/10.1186/1756-3305-6-257
  6. AbuBakar S, Teoh BT, Sam SS, Chang LY, Johari J, Hooi PS, Lakhbeer-Singh HK, Italiano CM, Syed Omar SF, Wong KT, Ramli N, Tan CT. Outbreak of human infection with Sarcocystis nesbitti, Malaysia, 2012. Emerg Infect Dis 2013; 19: 1989-1991. https://doi.org/10.3201/eid1912.120530
  7. Esposito DH, Stich A, Epelboin L, Malvy D, Han PV, Bottieau E, da Silva A, Zanger P, Slesak G, van Genderen PJ, Rosenthal BM, Cramer JP, Visser LG, Munoz J, Drew CP, Goldsmith CS, Steiner F, Wagner N, Grobusch MP, Plier DA, Tappe D, Sotir MJ, Brown C, Brunette GW, Fayer R, von Sonnenburg F, Neumayr A, Kozarsky PE. Acute muscular sarcocystosis: an international investigation among ill travelers returning from Tioman Island, Malaysia, 2011-2012. Clin Infect Dis 2014; 59: 1401-1410. https://doi.org/10.1093/cid/ciu622
  8. Shahari S, Tengku-Idris TIN, Fong MY, Lau YL. Molecular evidence of Sarcocystis nesbitti in water samples of Tioman Island, Malaysia. Parasit Vectors 2016; 9: 598. https://doi.org/10.1186/s13071-016-1883-9
  9. Thomas V, Dissanaike AS. Antibodies to Sarcocystis in Malaysians. Trans R Soc Trop Med Hyg 1978; 72: 303-306. https://doi.org/10.1016/0035-9203(78)90211-0
  10. Arness MK, Brown JD, Dubey JP, Neafie RC, Granstrom DE. An outbreak of acute eosinophilic myositis attributed to human Sarcocystis parasitism. Am J Trop Med Hyg 1999; 61: 548-553. https://doi.org/10.4269/ajtmh.1999.61.548
  11. Italiano CM, Wong KT, AbuBakar S, Lau YL, Ramli N, Syed Omar SF, Bador MK, Tan CT. Sarcocystis nesbitti causes acute, relapsing febrile myositis with a high attack rate: description of a large outbreak of muscular sarcocystosis in Pangkor Island, Malaysia, 2012. PLoS Negl Trop Dis 2014; 8: e2876. https://doi.org/10.1371/journal.pntd.0002876
  12. Habeeb YSM, Selim MA, Ali MS, Mahmoud LA, Hadi AMA, Shafei A. Serological diagnosis of extraintestinal sarcocystosis. J Egypt Soc Parasitol 1996; 26: 393-400.
  13. Abdul-Rahman SM, Rashad SM, Doma MA. Human muscle sarcocystosis in relation to non-specific rheumatic diseases and rheumatoid arthritis. Egypt Rheumatol Rehabil 2002; 29: 743-753.
  14. Mineo JR, Kasper LH. Attachment of Toxoplasma gondii to host cells involves major surface protein SAG-1(P30). Exp Parsitol 1994; 79: 11-20. https://doi.org/10.1006/expr.1994.1054
  15. Kim SK, Boothroyd JC. Stage-specific expression of surface antigens by Toxoplasma gondii as a mechanism to facilitate parasite persistence. J Immunol 2005; 174: 8038-8048. https://doi.org/10.4049/jimmunol.174.12.8038
  16. Liang FT, Granstrom DE, Zhao XM, Timoney JF. Evidence that surface proteins Sn14 and Sn16 of Sarcocystis neurona merozoites are involved in infection and immunity. Infect Immun 1998; 66: 1834-1838. https://doi.org/10.1128/iai.66.5.1834-1838.1998
  17. Tengku-Idris TIN, Fong MY, Lau YL. Seroprevalence of sarcocystosis in the local communities of Pangkor and Tioman Islands using recombinant surface antigens 3 (rSAG3) of Sarcocystis falcatula. Trop Med Int Health 2018; 23: 1374-1383. https://doi.org/10.1111/tmi.13160
  18. Wendte JM, Miller MA, Nandra AK, Peat SM, Crosbie PR, Conrad PA, Grigg ME. Limited genetic diversity among Sarcocystis neurona strains infecting southern sea otters precludes distinction between marine and terrestrial isolates. Vet Parasitol 2010; 169: 37-44. https://doi.org/10.1016/j.vetpar.2009.12.020
  19. Monteiro RM, Keid LB, Richtzenhain LJ, Valadas SY, Muller G, Soares RM. Extensively variable surface antigens of Sarcocystis spp. infecting Brazilian marsupials in the genus Didelphis occur in myriad allelic combinations, suggesting sexual recombination has aided their diversification. Vet Parasitol 2013; 196: 64-70. https://doi.org/10.1016/j.vetpar.2013.01.019
  20. Husna Maizura AM, Khebir V, Chong CK, Azman Shah AM, Azri A, Lokman Hakim S. Surveillance for sarcocystosis in Tioman Island, Malaysia. Malaysian J Pub Health Med 2012; 12: 39-44.
  21. Ahmad AF, Ngui R, Muhammad Aidil R, Lim YA, Rohela M. Current status of parasitic infections among Pangkor Island community in Peninsular Malaysia. Trop Biomed 2014; 31: 836-843.