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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Traction force microscopy for understanding cellular mechanotransduction

  • Hur, Sung Sik (Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University) ;
  • Jeong, Ji Hoon (Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University) ;
  • Ban, Myung Jin (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Soonchunhyang University) ;
  • Park, Jae Hong (Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Soonchunhyang University) ;
  • Yoon, Jeong Kyo (Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University) ;
  • Hwang, Yongsung (Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University)
  • Received : 2019.12.03
  • Published : 2020.02.29
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Abstract

Under physiological and pathological conditions, mechanical forces generated from cells themselves or transmitted from extracellular matrix (ECM) through focal adhesions (FAs) and adherens junctions (AJs) are known to play a significant role in regulating various cell behaviors. Substantial progresses have been made in the field of mechanobiology towards novel methods to understand how cells are able to sense and adapt to these mechanical forces over the years. To address these issues, this review will discuss recent advancements of traction force microscopy (TFM), intracellular force microscopy (IFM), and monolayer stress microscopy (MSM) to measure multiple aspects of cellular forces exerted by cells at cell-ECM and cell-cell junctional intracellular interfaces. We will also highlight how these methods can elucidate the roles of mechanical forces at interfaces of cell-cell/cell-ECM in regulating various cellular functions.

Keywords

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