Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Brucella melitensis omp31 Mutant Is Attenuated and Confers Protection Against Virulent Brucella melitensis Challenge in BALB/c Mice

  • Verdiguel-Fernandez, L (Laboratorio de Microbiologia Molecular, Departamento de Microbiologia e Inmunologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico) ;
  • Oropeza-Navarro, R (Departamento de Microbiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico) ;
  • Ortiz, Adolfo (Unidad de Bioseguridad de Brucella, Departamento de Microbiologia e Inmunologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico) ;
  • Robles-Pesina, MG (Centro Nacional de Servicios de Diagnostico en Salud Animal (CENASA), Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria) ;
  • Ramirez-Lezama, J (Departamento de Patologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico) ;
  • Castaneda-Ramirez, A (Departamento de Zootecnia, Universidad Autonoma de Chapingo) ;
  • Verdugo-Rodriguez, A (Laboratorio de Microbiologia Molecular, Departamento de Microbiologia e Inmunologia, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autonoma de Mexico)
  • Received : 2019.08.29
  • Accepted : 2020.01.12
  • Published : 2020.04.28
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Abstract

For control of brucellosis in small ruminants, attenuated B. melitensis Rev1 is used but it can be virulent for animals and human. Based on these aspects, it is essential to identify potential immunogens to avoid these problems in prevention of brucellosis. The majority of OMPs in the Omp25/31 family have been studied because these proteins are relevant in maintaining the integrity of the outer membrane but their implication in the virulence of the different species of this genus is not clearly described. Therefore, in this work we studied the role of Omp31 on virulence by determining the residual virulence and detecting lesions in spleen and testis of mice inoculated with the B. melitensis LVM31 mutant strain. In addition, we evaluated the conferred protection in mice immunized with the mutant strain against the challenge with the B. melitensis Bm133 virulent strain. Our results showed that the mutation of omp31 caused a decrease in splenic colonization without generating apparent lesions or histopathological changes apparent in both organs in comparison with the control strains and that the mutant strain conferred similar protection as the B. melitensis Rev1 vaccine strain against the challenge with B. melitensis Bm133 virulent strain. These results allow us to conclude that Omp31 plays an important role on the virulence of B. melitensis in the murine model, and due to the attenuation shown by the strain, it could be considered a vaccine candidate for the prevention of goat brucellosis.

Keywords

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