Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model

Dexamethasone으로 유도된 근위축 세포모델에서 glucoraphanin의 효과

  • Jeon, Sang Kyu (College of Korean Medicine, Dongguk University) ;
  • Kim, Ok Hyeon (College of Korean Medicine, Dongguk University) ;
  • Park, Su Mi (College of Korean Medicine, Dongguk University) ;
  • Lee, Ju-Hee (College of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (College of Korean Medicine, Dongguk University)
  • Received : 2020.02.16
  • Accepted : 2020.02.22
  • Published : 2020.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Glucoraphanin is one of the well-known natural glucosinolates found in cruciferous plants. In the present study, we investigated the effects and molecular mechanism of glucoraphanin in dexamethasone-induced skeletal muscle atrophy in vitro model. Methods : The cytotoxic effects of glucoraphanin on C2C12 myoblasts or myotubes were evaluated by MTT assay. The glucoraphanin was evaluated effects in dexamethasone-induced skeletal muscle atrophy in C2C12 myotubes using a real-time PCR, western blots analysis, and immunofluorescence staining of myosin heavy chain. Result : Glucoraphanin had no cytotoxicity on both C2C12 myoblasts or myotubes. Dexamethasone markedly induced muscle atrophy by up-regulating muscle-specific ubiquitin E3 ligase markers, atrogin-1 and MuRF1, and down-regulating MyoD, a myogenic regulatory factor whereas co-treatment of glucoraphanin and dexamethasone dose-dependently inhibited it. Furthermore, decreased expressions of p-Akt, p-FOXO1, and p-FOXO3a induced by dexamethasone were reversed by co-treatment with glucoraphanin and dexamethasone. In addition, dexamethasone obviously reduced myotube diameters, while co-treatment of glucoraphanin and dexamethasone increased those to a similar level as control. Conclusions : These results show that glucoraphanin suppresses dexamethasone-induced muscle atrophy in C2C12 myotubes through activation of Akt/FOXO signaling pathway.

Keywords

References

  1. Choe MA. Steroid induced muscle atrophy. Perspectives in Nursing Science 2005;2(1):19-36.
  2. Fanzani A, Conraads VM, Penna F, Martinet W. Molecular and cellular mechanisms of skeletal muscle atrophy: an update. J Cachexia Sarcopenia Muscle 2012;3(3):163-79. https://doi.org/10.1007/s13539-012-0074-6
  3. Hwang YS. Recent Advance in Diagnosis of Muscle Disease. J Korean Med Assoc 1995;38(6):719-26.
  4. Simmons Z. Management strategies for patients with amyotrophic lateral sclerosis from diagnosis through death. Neurologist 2005;11(5):257-70. https://doi.org/10.1097/01.nrl.0000178758.30374.34
  5. Jagoe, R. T. and Goldberg, A. L. 2001. What do we really know about the ubiquitinproteasome pathway in muscle atrophy? Curr. Opin. Clin. Nutr. Metab. Care 4, 183-190. https://doi.org/10.1097/00075197-200105000-00003
  6. Kandarian, S. C. and Jackman, R. W. 2006. Intracellular signaling during skeletal muscle atrophy. Muscle Nerve 33, 155-165. https://doi.org/10.1002/mus.20442
  7. Menconi M, Gonnella P, Petkova V, Lecker S, Hasselgren PO. Dexamethasone and corticosterone induce similar, but not identical, muscle wasting responses in cultured L6 and $C_2C_{12}$ myotubes. J Cell Biochem 2008;105:353-64. https://doi.org/10.1002/jcb.21833
  8. Park C, Jeong JW, Choi YH. Induction of muscle atrophy by dexamethasone and hydrogen peroxide in differentiated $C_2C_{12}$ myotubes. J Life Sci 2017;27(12):1479-85. https://doi.org/10.5352/JLS.2017.27.12.1479
  9. Srinibas, D., K.T. Amrish, and K. Harjit. 2000. Cancer modulation by glucosinolates: A review. Current Science 79:1665-1671.
  10. Im SB, Lee SH, Kim YY, Kim JS, Kim DS, Lim YP. Construction of a full-length cDNA library from Cardamine manshurica Nakai and characterization of EST dataset. Korean J Agric Sci 2016;43:33-9. https://doi.org/10.7744/kjoas.20160005
  11. Abdull Razis AF, Noor NM. Naturallyoccurring glucosinolates, glucoraphanin and glucoerucin, are antagonists to aryl hydrocarbon receptor as their chemopreventive potency. Asian Pac J Cancer Prev 2015;16(14):5801-5. https://doi.org/10.7314/APJCP.2015.16.14.5801
  12. Melrose J. The Glucosinolates: A sulphur glucoside family of mustard anti-tumour and antimicrobial phytochemicals of potential therapeutic application. Biomedicines 2019;7(3):62. https://doi.org/10.3390/biomedicines7030062
  13. Hong JY, Nam YJ, Kim MO, Kim HY. Discover bio future promising technologies in 2019. Biotech Policy Research Center. 2019:271(34).
  14. Kang YS, Park C, Han MH, Hong SH, Hwang HJ, Kim BW et al. Ethanol Extract of Schisandra chinensis (Turcz.) Baill. Reduces AICAR-induced Muscle Atrophy in $C_2C_{12}$ Myotubes. J Life Sci 2015;25(3):293-8. https://doi.org/10.5352/JLS.2015.25.3.293
  15. Kim Y, Kim CS, Joe Y, Chung HT, Ha TY, Yu R. Quercetin Reduces Tumor Necrosis Factor Alpha-Induced Muscle Atrophy by Upregulation of Heme Oxygenase-1. J Med Food 2018;21(6):551-9. https://doi.org/10.1089/jmf.2017.4108
  16. Sakai H, Kimura M, Tsukimura Y, Yabe S, Isa Y, Kai Y et al. Dexamethasone exacerbates cisplatin-induced muscle atrophy. Clin Exp Pharmacol Physiol 2019;46(1):19-28. https://doi.org/10.1111/1440-1681.13024
  17. Baek SJ, Kim DH, Kim JS. Review of the muscle plasticity. J Korean Soc Phys Ther 2003;15(2):239-47.
  18. Bentzinger CF, Wang YX, Rudnicki MA. Building muscle: molecular regulation of myogenesis. Cold Spring Harb Perspect Biol. 2012;4(2). pii: a008342.
  19. Song MY. Effect of Root of Atractylodes macrocephala Koidzumi on myogenesis in $C_2C_{12}$ cells. J Korean Med Obes Res 2015;15:38-44. https://doi.org/10.15429/jkomor.2015.15.1.38
  20. Schiaffino S, Dyar KA, Ciciliot S, Blaauw B, Sandri M. Mechanisms regulating skeletal muscle growth and atrophy. FEBS J 2013;280(17):4294-314. https://doi.org/10.1111/febs.12253
  21. Choi AR, Lee GS, Chae HJ. Effects of ultrasonication on the analysis of sulforaphane content in vegetables. J Korea Acad Industr Coop Soc 2008;9(3):794-9. https://doi.org/10.5762/KAIS.2008.9.3.794
  22. Shin MK. Clinical Traditional Herbalogy. Seoul:Yeong Lim's Publisher. 2000.