Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Identification of 12 radiation-resistant bacterial species in the phylum Proteobacteria new to Korea

  • Han, Joo Hyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Maeng, Soohyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Park, Yuna (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Sang Eun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Byoung-Hee (Environmental Research Complex, National Institute of Biological Resources) ;
  • Lee, Ki-eun (Environmental Research Complex, National Institute of Biological Resources) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2020.01.02
  • Accepted : 2020.02.17
  • Published : 2020.05.31
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Abstract

In 2019, after a comprehensive investigation of indigenous prokaryotic species in Korea, a total of 12 bacterial strains assigned to the phylum Proteobacteria were isolated from soil. With the high 16S rRNA gene sequence similarity (>98.8%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to independent, predefined bacterial species. This study identified two species in the family Burkholderiaceae, one species in the family Comamonadaceae, two species in the family Oxalobacteraceae, one species in the family Micrococcaceae, one species in the family Bradyrhizobiaceae, one species in the family Methylobacteriaceae, one species in the family Rhizobiaceae, one species in the family Rhodocyclaceae, and one species in the family Sphingomonadaceae. There is no official report about these 12 species in Korea, so are described as unreported bacterial species in Korea in this study. Gram reaction, basic biochemical characteristics, colony, and cell morphology are also described in the species description section.

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References

  1. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
  2. Garrity, G.M., J.A. Bell and T. Lilburn. 2005. Phylum XIV. Proteobacteria phyl. nov. In: Bergey's Manual of Systematic Bacteriology Springer, New York, NY.
  3. Gupta, R.S. 2000. The phylogeny of proteobacteria: relationships to other eubacterial phyla and eukaryotes. FEMS Microbiology Review 24(4):367-402. https://doi.org/10.1111/j.1574-6976.2000.tb00547.x
  4. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series Information Retrieval Ltd, London.
  5. Hirsch, P., W. Ludwig, C. Hethke, M. Sittig, B. Hoffmann and C.A. Gallikowski. 1998. Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antartica soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Systamatic Applied Microbiology 21:374-383. https://doi.org/10.1016/S0723-2020(98)80047-7
  6. Im, W.T., H.M. Jung, L.N. Ten, M.K. Kim, N. Bora, M. Goodfellow, S. Lim, J. Jung and S.T. Lee 2008. Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge. International Journal of Systematic and Evolutionary Microbiology 58:2348-2353. https://doi.org/10.1099/ijs.0.64082-0
  7. Kersters, K., P. De Vos, M. Gillis, J. Swings, P. Vandamme and E. Stackebrandt. 2006. Introduction to the Proteobacteria. In: The Prokaryotes Springer, New York, NY.
  8. Kim, O.S., Y.J. Cho, K. Lee, S.H. Yoon, M. Kim, H. Na, S.C. Park, Y.S. Jeon, J.H. Lee, H. Yi, S. Won and J. Chun 2012. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. International Journal of Systematic and Evolutionary Microbiology 62:716-721. https://doi.org/10.1099/ijs.0.038075-0
  9. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  10. Kumar, S., G. Stecher and K. Tamura 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datadets. Molecular Biology and Evolution 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  11. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  12. Selvam, K., J.R. Duncan, M. Tanaka and J.R. Battista. 2013. DdrA, DdrD, and PprA: components of UV and mitomycin C resistance in Deinococcus radiodurans R1. PLoS One 8 (7):e69007. https://doi.org/10.1371/journal.pone.0069007
  13. Stackebrandt, E., R.G.E. Murray and H.G. Truper. 1988b Proteobacteria classis nov., a name for the phylogenetic taxon that includes the "purple bacteria and their relatives." Int. J. Syst. Bacteriol. 38 321-325. https://doi.org/10.1099/00207713-38-3-321
  14. Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J Lane 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173:697-703. https://doi.org/10.1128/JB.173.2.697-703.1991
  15. Woese, CR. 1987. “Bacterial evolution”. Microbiological Reviews 51(2):221-271. https://doi.org/10.1128/MMBR.51.2.221-271.1987
  16. Wragg, P., L. Randall and A.M. Whatmore 2014. Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flightmass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest. Journal of Microbiological Methods 105:16-21. https://doi.org/10.1016/j.mimet.2014.07.003