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Dark Field Digital Holographic Microscopy Based on Two-lens 360-degree Oblique Illumination

  • Zhang, Xiuying (Institute of Advanced Materials, Nanjing Tech University) ;
  • Zhao, Yingchun (Changzhou Xingyu Automotive Lighting System Co. Ltd.) ;
  • Yuan, Caojin (Key Laboratory for Opto-Electronic Technology of Jiangsu Province, Nanjing Normal University) ;
  • Feng, Shaotong (Key Laboratory for Opto-Electronic Technology of Jiangsu Province, Nanjing Normal University) ;
  • Wang, Lin (School of Electronic and Optical Engineering, Nanjing University of Science and Technology)
  • Received : 2019.10.31
  • Accepted : 2020.03.15
  • Published : 2020.06.25

Abstract

In this paper we propose a dark-field digital holographic microscopy system based on 360-degree oblique illumination. This setup is constructed without using a dark-field objective. The principle of 360-degree oblique illumination of vortex beam and dark-field digital holographic microscopy are introduced theoretically and experimentally. By analyzing the reconstructed image of a dark-field digital hologram of a USAF 1951 target, it is proved that the imaging resolution can be improved by this method. And also, comparison and analysis are made on the reconstructed image of a bright-dark field digital hologram of a pumpkin stem slice, the result shows that the imaging contrast is also enhanced with this method, and it is effective for dark-field digital holographic microscopy imaging of large transparent biological samples.

Keywords

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