DOI QR코드

DOI QR Code

Phenolic compounds in domestic and imported grape cultivars in Korea

국내산 포도와 수입산 포도의 페놀계화합물 함량

  • Jung, Sung Min (Planing and Coordination Division, National Institute of Horticultural and Herbal Science) ;
  • Kim, Su Jin (Fruit Research Division, National Institute of Horticultural and Herbal Science) ;
  • Hur, Youn Young (Fruit Research Division, National Institute of Horticultural and Herbal Science)
  • 정성민 (국립원예특작과학원 기획조정과) ;
  • 김수진 (국립원예특작과학원 과수과) ;
  • 허윤영 (국립원예특작과학원 과수과)
  • Received : 2020.03.07
  • Accepted : 2020.04.15
  • Published : 2020.06.30

Abstract

In this study, fruit phenolics were investigated with using LC/MS and HPLC analysis in order to compare the differences between domestic ('Campbell Early', 'Kyoho', 'Heukbosuk', and 'Hongju SDS') and imported ('Crimson SDS' and 'Thomson SDS') grapes. In the case of fruit characteristics, imported grape 'Crimson SDS' and 'Thompson SDS', had lighter skin weight (300-350 mg/berry) and hard flesh (5.2-5.6 kg·f) than domestic grape cultivar. The phenolic compound contents of 'Crimson SDS' skin was higher, but resveratrol (25-29 mg/kg), quercetin (350-380 mg/kg), and myricetin (31-32 mg/kg) contents were similar in to those of 'Hongju SDS'. The anthocyanin content was different from differed between grape cultivars. 'Hongju SDS' grape was showed higher in Delphinidin-3-glucose (D3G) levels, and 'Crimson SDS' was showed higher in Peonidin-3-glucoside (P3G) levels. The contents of phenolic compounds were investigated differently for each grape berry part. Catechin, epicatechin, procyanidin B1, and B2 were found in grape seeds.

국내산 포도 품종 'Campbell Early', 'Kyoho', 'Heukbosuk', 'Hongju Seedless (SDS)'와 수입 포도 'Crimson SDS', 'Thomson SDS'의 페놀계 화합물을 비교하기 위해 LC-MS/MS와 HPLC를 이용하여 함량을 비교하였다. 수입포도 'Crimson SDS'와 'Thompson SDS' 품종은 국내산 포도 품종보다 가벼운 과피무게(300-350 mg)와 단단한 경도(5.2-5.6 kg·f)를 가진 것으로 조사되었다. 'Crimson SDS' 품종의 과피에 함유된 페놀계 화합물 함량은 다른 포도 품종에 비해 전반적으로 높았지만, resveratrol (25-29 mg/kg), quercetin (350-380 mg/kg), myricetin (31-32 mg/kg) 함량은 'Hongju SDS' 품종과 유사하였다. Anthocyanin 함량은 포도 품종에 따라 달랐는데, 'Hongju SDS' 품종은 Delphinidin-3-glucoside의 함량이 높았고, 'Crimson SDS' 품종은 Peonidin-3-glucoside의 함량이 높았다. 페놀 화합물의 함량은 포도 부위별 다른 함량을 나타냈고, catechin, epicatechin, procyanidin B1, B2는 포도 종자에서 전형적인 화합물이었다. 과피를 함께 먹을 수 있는 포도품종은 과피에 포함된 유용한 phenolic compounds를 효과적으로 이용할 수 있다.

Keywords

References

  1. Buchanan BB, Gruissem W, Jones RL. Biochemistry and molecular biology of plants. American society of plant biologist, Rockville, Maryland, USA (2000)
  2. Burin VM, Ferreira-Lima NE, Panceri CP, Bordignon-Luiz MT. Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis labrusca grapes: Evaluation of different extraction methods. Microchem. J. 114: 155-163 (2014) https://doi.org/10.1016/j.microc.2013.12.014
  3. Cai Y, Yu Y, Duan G, Li Y. Study on infrared-assisted extraction coupled with high performance liquid chromatography (HPLC) for determination of catechin, epicatechin, and procyanidin B2 in grape seeds. Food Chem. 127: 1872-1877 (2011) https://doi.org/10.1016/j.foodchem.2011.02.026
  4. Carreno J, Almela L, Martinez A, Lopez JAF. Chemotaxonomy classification of red table grapes based on anthocyanin profile and external colour. Food Sci. Technol. 30: 259-265 (1997)
  5. Castellarin S, Gaspero G. Transcriptional control of anthocyanin biosynthesis genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines. BMC Plant Biol. 7: 46 (2007) https://doi.org/10.1186/1471-2229-7-46
  6. Chang EH, Jung SM, Park KS, Lim BS. Contents of phenolic compounds and trans-resveratrol in different parts of Korean new grape cutivars. Kor. J. Food Sci. Techonl. 45: 708-713 (2013) https://doi.org/10.9721/KJFST.2013.45.6.708
  7. Chang SW, Kim HJ, Song JH, Lee KY, Kim IH, Rho YT. Determination of several phenolic compounds in cultivars of grape in Korea. Korean J. Food Preserv. 18: 328-334 (2011) https://doi.org/10.11002/kjfp.2011.18.3.328
  8. Choi JY, Cho EK, Park SJ, Hur YY, Nam JC, Koh SW, Jung SM. Application of color index for red grapes (CIRG) for assessment of grape quality. Protected Hort. Plant Fac. 23: 244-249 (2014) https://doi.org/10.12791/KSBEC.2014.23.3.244
  9. De Rosso M, Panighel A, Vedova AD, Gardiman M, Flamini R. Characterization of non-anthocyanic flavonoids in some hybrid red grape extracts potentially interesting for industrial uses. Molecules, 20: 18095-18106 (2015) https://doi.org/10.3390/molecules201018095
  10. Devi KP, Rajavel T, Habtemariam S, Nabavi SF, Nabavi SM. Molecular mechanisms underlying anticancer effects of myricetin. Life Sci. 142: 19-25 (2015) https://doi.org/10.1016/j.lfs.2015.10.004
  11. Gehm BD, McAndrews JM, Chien PY, Jameson JL. Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Proc. Natl. Acad. Sci. 94: 14138-14143 (1997) https://doi.org/10.1073/pnas.94.25.14138
  12. Han F, Yang P, Wang H, Fernandes I, Mateus N, Liu Y. Digestion and absorption of red grape and wine anthocyanins through the gastrointestinal tract. Trends in Food Sci. Technol. 83: 211-224 (2019) https://doi.org/10.1016/j.tifs.2018.11.025
  13. Iacopini P, Baldi M, Storchi P, Sebastiani L. Catechin, epicatechin, quercetin, rutin and resveratrol in red grape: Content in vitro antioxidant activity and interactions. J. Food Compos. Anal. 21: 589-598 (2008) https://doi.org/10.1016/j.jfca.2008.03.011
  14. OIV (International Organization of Vine and Wine). 2019 Statistical report on world vitiviniculture. OIV, Paris, France (2019)
  15. Jackson R, Wine Science. Academic Press. (2014)
  16. Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CWW, Fong HHS, Farnsworth NR, Kinghorn D, Mehta RG, Moon RC, Pezzuto JM. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275: 218-220 (1997) https://doi.org/10.1126/science.275.5297.218
  17. Jung SM, Park KS, Roh JH, Lee SC, Park JM, Hur YY, Cho MA, Nam JC. Top-fruit production manual-grape. Rural Development Administration (2012)
  18. KCS (Korea Customs Service). Trade statistics. KCS, Daejeon, Korea (2019)
  19. Kim ES, Chang EH, Hur YY, Kim TW, Jung SM. Anthocyanin contents and composition of VlmybA1-2 and VlmybA2 genes in Vitis labrusca hybrid grape cultivars and cross seedlings. Plant Omics J. 8: 472-478 (2015)
  20. Lee GA, Choi KC, Hwang KA. Kaempferol, a phytoestrogen, suppressed triclosan-induced epithelial-mesenchymal transition and metastatic-related behaviors of MCF-7 breast cancer cells. Environ. Toxicol. Pharmacol. 49: 48-57 (2017) https://doi.org/10.1016/j.etap.2016.11.016
  21. Perez-Navarro J, Izquierdo-Caas PM, Mena-Morales A, Martnez-Gascuea J, Chacn-Vozmediano JL, Garca-Romero E, Hermosn-Gutirrez I, Gmez-Alonso S, Phenolic compounds profile of different berry parts from novel Vitis vinifera L. red grape genotypes and Tempranillo using HPLC-DAD-ESI-MS/MS: A varietal differentiation tool, Food Chem. 295: 350-360 (2019) https://doi.org/10.1016/j.foodchem.2019.05.137
  22. Price SF, Breen PJ, Valladao M, Watson BT. Cluster sun exposure and quercetin in Pinot noir grape and wine. Am. J. Enol. Vitic. 46: 187-194 (1995)
  23. Rivero-Perez MD, Muniz P, Gonzalez-Sanjose ML. Contribution of anthocyanin fraction to the antioxidant properties of wine. Food Chem. Toxicol. 46: 2815-2822 (2008) https://doi.org/10.1016/j.fct.2008.05.014
  24. Rodriguez-Vaquero MJ, Alberto MR, Manca-de-Nadra MC. Antibacterial effect of phenolic compounds from different wines. Food Control 18: 93101 (2007)
  25. Roh JH, Hur YY, Jung SM, Park KS, Nam JC, Hwant HS, Im DJ, Chung KH, Yun HK. 'Hongju': A seedless table grape cultivar. HortScience 53: 1909-1910 (2018) https://doi.org/10.21273/HORTSCI13368-18
  26. Sarah SL, McFaland M, Niles RM. Molecular and physiological actions of quercetin: need for clinical trials to assess its benefits in human disease. Nutr. Rev. 72: 720-734 (2014) https://doi.org/10.1111/nure.12152
  27. Singleton VL, Zaya J, Trousdale EK. Caftaric and coutaric acids in fruit of Vitis. Phytochem. 25: 2127-2133 (1986) https://doi.org/10.1016/0031-9422(86)80078-4
  28. Sun ZJ, Chen G, Hu X, Zhang W, Liu Y, Zhu LX, Zhou Q, Zhao YF. Activation of PI3K/Akt/IKK-alpha/NF-kappaB signaling pathway is required for the apoptosis-evasion in human salivary adenoid cystic carcinoma: its inhibition by quercetin. Apoptosis, 15: 850-863 (2010) https://doi.org/10.1007/s10495-010-0497-5
  29. Xia EQ, Deng GF, Guo YJ, Li HB. Biological activies of polyphenols from grapes. Int. J. Mol. Sci. 11: 622-646 (2010) https://doi.org/10.3390/ijms11020622