Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Resource conservation using whole body autophagy: Self-digestion of shedded gut lining cells in the small intestine

  • Lee, Phil Jun (College of Pharmacy and Ajou University) ;
  • Cho, Namki (College of Pharmacy and Research Institute of Drug Development, Chonnam National University) ;
  • Yoo, Hee Min (Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS)) ;
  • Chang, Sun-Young (College of Pharmacy and Ajou University) ;
  • Ko, Hyun-Jeong (Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University) ;
  • Kim, Hong Pyo (College of Pharmacy and Ajou University)
  • Received : 2020.03.10
  • Accepted : 2020.04.14
  • Published : 2020.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

To retain valuable resources, organisms adopt several strategies including coprophagy. Cells covering the outer skin and internal digestive lumen are actively recycled to maintain their integrity. In present study, we suggested that the small intestine can consume dead cells in a manner similar to how it consumes protein from the diet. We examined the eluates from five segments of the mouse small intestine and cecum and 2 segments of the large intestine and small intestine tissue, and detected immunoreactivity with eukaryotic caveolin-1 and β-actin antibodies only in the cecum and 2 segments from the large intestine. Bacterial agitation of the mouse intestine with Shigella disrupted the architecture and absorptive function of the small intestine. Small intestine eluates were immunoreactive with murine caveolin-1 and contained heme as determined by dot blot analysis. We concluded that the body conserves resources in the small intestine by disposing of and recycling shedded cells.

Keywords

References

  1. Bullen TF, Forrest S, Campbell F, Dodson AR, Hershman MJ, Pritchard DM, Turner JR Montrose MH, Watson AJ. Characterization of epithelial cell shedding from human small intestine. Lab. Invest. 86: 1052-1063 (2006) https://doi.org/10.1038/labinvest.3700464
  2. Chang SY, Lee SN, Yang JY, Kim DW, Yoon JH, Ko HJ, Ogawa M, Sasakawa C, Kweon MN Autophagy controls an intrinsic host defense to bacteria by promoting epithelial cell survival: A murine model. PLoS One 8: e81095 (2013) https://doi.org/10.1371/journal.pone.0081095
  3. Cummings JH, Macfarlane GT. Role of intestinal bacteria in nutrient metabolism. JPEN J. Parenter. Enteral Nutr. 21: 357-365 (1997) https://doi.org/10.1177/0148607197021006357
  4. Hausmann M. How bacteria-induced apoptosis of intestinal epithelial cells contributes to mucosal inflammation. Int. J. Inflam. 2010: 574568 (2010)
  5. Iwanaga T, Han H, Adachi K, Fujita T. A novel mechanism for disposing of effete epithelial cells in the small intestine of guinea pigs. Gastroenterology 5: 1089-1097 (1993) https://doi.org/10.1016/0016-5085(93)90953-A
  6. Jeon JW, Lee JI, Shin HP, Cha JM, Joo KR, Kim SH. Adenosine A2A-receptor agonist polydeoxyribonucleotide promotes gastric ulcer healing in Mongolian gerbils. Animal Cells Syst. 18: 399-406 (2014) https://doi.org/10.1080/19768354.2014.983968
  7. Jin Y, Kim HP, Cao J, Zhang M, Ifedigbo E, Choi AM. Caveolin-1 regulates the secretion and cytoprotection of Cyr61 in hyperoxic cell death. FASEB J. 23: 341-350 (2009) https://doi.org/10.1096/fj.08-108423
  8. Lee PJ, Cho N, Yoo HM, Kim HP. Active turnover of heme in hibernation period in mammals. Front Physiol. 10: 1586 (2020) https://doi.org/10.3389/fphys.2019.01586
  9. Lee PJ, Park HJ, Cho N, Kim HP. 3,5-Diethoxy-3'-Hydroxyresveratrol (DEHR) ameliorates liver fibrosis via caveolin-1 activation in hepatic stellate cells and in a mouse model of bile duct ligation injury. Molecules 23: 2833 (2018) https://doi.org/10.3390/molecules23112833
  10. Lee PJ, Woo SJ, Jee JG, Sung SH, Kim HP. Bisdemethoxycurcumin induces apoptosis in activated hepatic stellate cells via cannabinoid receptor 2. Molecules 20: 1277-1292 (2015) https://doi.org/10.3390/molecules20011277
  11. Lin J. Redies C. Histological evidence: housekeeping genes betaactin and GAPDH are of limited value for normalization of gene expression. Dev. Genes Evol. 222: 369-376 (2012) https://doi.org/10.1007/s00427-012-0420-x
  12. Lodish H. Molecular Cell Biology. W. H. Freeman, New York, NY, USA (2000)
  13. Martin R. How we do it. The evolution and future of human reproduction. Basic Books, New York, NY, USA. pp. 118-119 (2011)
  14. Phillips R, Milo R. Cell Biology by the Numbers. Garland Science, Boca Raton, FL, USA (2015):
  15. Pritchard DM, Potten CS, Korsmeyer SJ, Roberts S, Hickman JA. Damage-induced apoptosis in intestinal epithelia from bcl-2-null and bax-null mice: investigations of the mechanistic determinants of epithelial apoptosis in vivo. Oncogene 18: 7287-7293 (1999) https://doi.org/10.1038/sj.onc.1203150
  16. Warren MJ, Smith A. Tetrapyrroles: Birth, Life, and Death. Springer, Berlin, Germany (2009)
  17. Williams JM, Duckworth CA, Burkitt MD, Watson AJ, Campbell BJ, Pritchard DM. Epithelial cell shedding and barrier function: a matter of life and death at the small intestinal villus tip. Vet. Pathol. 52: 445-455 (2015) https://doi.org/10.1177/0300985814559404